Objective:To investigate the potential role of the regulator of G protein signaling 20(RGS20)in radioresistance of non-small cell lung cancer(NSCLC).Methods:A total of 35 lung adenocarcinoma(LUAD)patients from The Can...Objective:To investigate the potential role of the regulator of G protein signaling 20(RGS20)in radioresistance of non-small cell lung cancer(NSCLC).Methods:A total of 35 lung adenocarcinoma(LUAD)patients from The Cancer Genome Atlas(TCGA),who un-derwent radiotherapy,were enrolled and divided into radiosensitive(n=16)and radioresistant(n=19)groups based on clinical prognosis.The expression and prognosis of RGS20 were analyzed by Gene Expression Profiling Interactive Analysis(GEPIA)database.A radioresistant cell line(A549R)was constructed by irradiating A549 cells with 6 Gy X-rays for 10 fractions.Cell survival was measured by colony formation assay.The regulatory effect of RGS20 on pyroptosis were verified by LDH release and Western blot assay,and the underlying mech-anism was investigated by transfecting RGS20 siRNA and applying a GSDMD inhibitor.Results:A total of 2,181 differentially expressed genes(DEGs)were identified by analyzing the data of radio-sensitive and radioresistant individuals from the TCGA-LUAD dataset.These DEGs were enriched in G alpha(z)signalling events analyzed by Reactome database.RGS20 exhibited significant upregulation among the DEGs,and its higher expression predicted poor prognosis in LUAD patients.In vitro,the expression of RGS20 protein was increased by irradiation in A549 cells,whereas it remained at much high levels in A549R cells regardless of irradiation.After irradiation,the expressions of pyroptosis-related proteins were significantly increased in A549 cells(P<0.05),with no significant changes were observed in A549R cells.Treatment with LDC7559 significantly reduced LDH release(P<0.01)and improved the survival rate of irradiated A549 cells(P<0.01).Furthermore,knockdown of RGS20 gene in A549R cells significantly increased LDH release(P<0.001)and enhanced radiosensitivity(P<0.01),while LDC7559 administration reversed LDH release(P<0.01)and radiation-induced cell death increased by siRGS20(P<0.05).Meantime,the increased expression level of GSDMD-NT was observed in A549 and A549R cells transfected with siRGS20(P<0.05).Conclusion:RGS20 contributes to the radioresistance of NSCLC cells,which might be a potential target for NSCLC radiotherapy.展开更多
As a consumer product,black chokeberry(Aronia melanocarpa)is diffi cult to consume due to its pungent taste.It is mainly used in various food industries to produce a variety of juices,preserves,extracts,fruit teas and...As a consumer product,black chokeberry(Aronia melanocarpa)is diffi cult to consume due to its pungent taste.It is mainly used in various food industries to produce a variety of juices,preserves,extracts,fruit teas and nutritional supplements.Traditionally,Potawatomi Native Americans used black chokeberry to treat colds,coughs and fever.It has been found to contain numerous active ingredients.Currently,there are many health and medical products that contain black chokeberry.The purpose of this review is to understand the nutritional benefi ts of black chokeberry and to explore its benefi cial effects on biological systems in vitro,thereby emphasizing its therapeutic prospects.展开更多
Renewable paper reusing plays a significant role in the sustainable environment under the background of the shortage in forest resources and the pollution from the paper industry.The conventional reusing stream of was...Renewable paper reusing plays a significant role in the sustainable environment under the background of the shortage in forest resources and the pollution from the paper industry.The conventional reusing stream of waste office paper appears to have low reusing rates while consuming massive amounts of energy in intermediate steps.In this study,we developed a novel portable renewable desktop paper reusing system based on font area detection and greyscale sensor.The proposed system consists of two main parts,namely,a greyscale sensor and font area detection model and a polishing mechanism.Acting as an ink mark detector for waste desktop paper,the greyscale sensor and font area detection model can detect the font in the waste desktop paper using an adaptive dynamic compensation schematic.The polishing mechanism will grind the font area of the wasted desktop paper,and this paper reusing processing is non-chemical,energy saving and environmentally friendly.The proposed system is demonstrated through simulations and experimental results,which show that the proposed renewable desktop paper reusing system is portable and is effective for reusing waste office paper in the office.An accuracy of 99.78%is demonstrated in the greyscale sensor and font area detection model,and the average reuse rate of one piece of paper is 2.52 times,verifying that the proposed portable system is effective and practical in renewable desktop paper reusing applications.展开更多
基金funded by the National Natural Science Foundation of China(No.12235004,12175044,32171235,12375338).
文摘Objective:To investigate the potential role of the regulator of G protein signaling 20(RGS20)in radioresistance of non-small cell lung cancer(NSCLC).Methods:A total of 35 lung adenocarcinoma(LUAD)patients from The Cancer Genome Atlas(TCGA),who un-derwent radiotherapy,were enrolled and divided into radiosensitive(n=16)and radioresistant(n=19)groups based on clinical prognosis.The expression and prognosis of RGS20 were analyzed by Gene Expression Profiling Interactive Analysis(GEPIA)database.A radioresistant cell line(A549R)was constructed by irradiating A549 cells with 6 Gy X-rays for 10 fractions.Cell survival was measured by colony formation assay.The regulatory effect of RGS20 on pyroptosis were verified by LDH release and Western blot assay,and the underlying mech-anism was investigated by transfecting RGS20 siRNA and applying a GSDMD inhibitor.Results:A total of 2,181 differentially expressed genes(DEGs)were identified by analyzing the data of radio-sensitive and radioresistant individuals from the TCGA-LUAD dataset.These DEGs were enriched in G alpha(z)signalling events analyzed by Reactome database.RGS20 exhibited significant upregulation among the DEGs,and its higher expression predicted poor prognosis in LUAD patients.In vitro,the expression of RGS20 protein was increased by irradiation in A549 cells,whereas it remained at much high levels in A549R cells regardless of irradiation.After irradiation,the expressions of pyroptosis-related proteins were significantly increased in A549 cells(P<0.05),with no significant changes were observed in A549R cells.Treatment with LDC7559 significantly reduced LDH release(P<0.01)and improved the survival rate of irradiated A549 cells(P<0.01).Furthermore,knockdown of RGS20 gene in A549R cells significantly increased LDH release(P<0.001)and enhanced radiosensitivity(P<0.01),while LDC7559 administration reversed LDH release(P<0.01)and radiation-induced cell death increased by siRGS20(P<0.05).Meantime,the increased expression level of GSDMD-NT was observed in A549 and A549R cells transfected with siRGS20(P<0.05).Conclusion:RGS20 contributes to the radioresistance of NSCLC cells,which might be a potential target for NSCLC radiotherapy.
基金funded by the Key R&D Program of the Ministry of Science and Technology(SQ2020YFF0422819)Jilin Traditional Chinese Medicine Science and Technology Project(2020141).
文摘As a consumer product,black chokeberry(Aronia melanocarpa)is diffi cult to consume due to its pungent taste.It is mainly used in various food industries to produce a variety of juices,preserves,extracts,fruit teas and nutritional supplements.Traditionally,Potawatomi Native Americans used black chokeberry to treat colds,coughs and fever.It has been found to contain numerous active ingredients.Currently,there are many health and medical products that contain black chokeberry.The purpose of this review is to understand the nutritional benefi ts of black chokeberry and to explore its benefi cial effects on biological systems in vitro,thereby emphasizing its therapeutic prospects.
基金This work was supported by the National Natural Science Founda-tion of China under Grant Nos.51675451 and 51975490by the Science and Technology Projects of Sichuan under Grant Nos.19MZGC0116,19MZGC0117 and 19MZGC0110.
文摘Renewable paper reusing plays a significant role in the sustainable environment under the background of the shortage in forest resources and the pollution from the paper industry.The conventional reusing stream of waste office paper appears to have low reusing rates while consuming massive amounts of energy in intermediate steps.In this study,we developed a novel portable renewable desktop paper reusing system based on font area detection and greyscale sensor.The proposed system consists of two main parts,namely,a greyscale sensor and font area detection model and a polishing mechanism.Acting as an ink mark detector for waste desktop paper,the greyscale sensor and font area detection model can detect the font in the waste desktop paper using an adaptive dynamic compensation schematic.The polishing mechanism will grind the font area of the wasted desktop paper,and this paper reusing processing is non-chemical,energy saving and environmentally friendly.The proposed system is demonstrated through simulations and experimental results,which show that the proposed renewable desktop paper reusing system is portable and is effective for reusing waste office paper in the office.An accuracy of 99.78%is demonstrated in the greyscale sensor and font area detection model,and the average reuse rate of one piece of paper is 2.52 times,verifying that the proposed portable system is effective and practical in renewable desktop paper reusing applications.
