[Objectives] By reasonable water distribution and air supply to soil, soil water permeability is maintained, and the nutrient conditions of degrading bacteria in soil are improved to effectively prevent soil clogging....[Objectives] By reasonable water distribution and air supply to soil, soil water permeability is maintained, and the nutrient conditions of degrading bacteria in soil are improved to effectively prevent soil clogging. [Methods] Through the innovation and improvement of traditional soil sewage treatment technology, the physical, chemical and biological characteristics of soil for sewage purification are utilized to enhance the pollutant decomposition ability of soil microorganisms and maintain soil water permeability.[Results] It has no secondary pollution, and can effectively remove pollutants such as COD_(Cr), TN, TP, NH_3-N, etc. in sewage. [Conclusions] The operation and maintenance cost is low, and the land can be reused, while water and nutrients can be restored to the land.展开更多
Force spectrum measurements with constant loading rates are widely used in single-molecule manipulation experiments to study the mechanical stability and force response of biomolecules.Force-dependent transition rates...Force spectrum measurements with constant loading rates are widely used in single-molecule manipulation experiments to study the mechanical stability and force response of biomolecules.Force-dependent transition rates can be obtained from the transition force distribution,but it is limited to the force range with non-zero force distribution.Although constant loading rate control can be realized with magnetic tweezers,the loading rate range is limited due to the slow movement of permanent magnets.Non-linear exponential and exponential squared force loading functions are more feasible in magnetic tweezers,while there is no theoretical result available for these two kinds of non-linear force loading functions.In this study,we solved the unfolding process of a protein following Bell's model under nonlinear exponential and exponential squared force loading functions,which offer a broader range of unfolding force distribution compared to the traditional constant loading rate experiments.Furthermore,we derived two force loading functions,which can produce uniform unfolding force distribution.This research contributes fundamental equations for the analysis of experimental data obtained through single-molecule manipulation under nonlinear force loading controls,paving the way for the use of nonlinear force control in magnetic tweezer experiments.展开更多
文摘[Objectives] By reasonable water distribution and air supply to soil, soil water permeability is maintained, and the nutrient conditions of degrading bacteria in soil are improved to effectively prevent soil clogging. [Methods] Through the innovation and improvement of traditional soil sewage treatment technology, the physical, chemical and biological characteristics of soil for sewage purification are utilized to enhance the pollutant decomposition ability of soil microorganisms and maintain soil water permeability.[Results] It has no secondary pollution, and can effectively remove pollutants such as COD_(Cr), TN, TP, NH_3-N, etc. in sewage. [Conclusions] The operation and maintenance cost is low, and the land can be reused, while water and nutrients can be restored to the land.
基金supported by the National Natural Science Foundation of China (Grant Nos. 12174322 to HC, 12204124 to ZG, 32271367 and 12204389 to SL)the 111 project (Grant No. B16029)the Research Fund of Wenzhou Institute
文摘Force spectrum measurements with constant loading rates are widely used in single-molecule manipulation experiments to study the mechanical stability and force response of biomolecules.Force-dependent transition rates can be obtained from the transition force distribution,but it is limited to the force range with non-zero force distribution.Although constant loading rate control can be realized with magnetic tweezers,the loading rate range is limited due to the slow movement of permanent magnets.Non-linear exponential and exponential squared force loading functions are more feasible in magnetic tweezers,while there is no theoretical result available for these two kinds of non-linear force loading functions.In this study,we solved the unfolding process of a protein following Bell's model under nonlinear exponential and exponential squared force loading functions,which offer a broader range of unfolding force distribution compared to the traditional constant loading rate experiments.Furthermore,we derived two force loading functions,which can produce uniform unfolding force distribution.This research contributes fundamental equations for the analysis of experimental data obtained through single-molecule manipulation under nonlinear force loading controls,paving the way for the use of nonlinear force control in magnetic tweezer experiments.
