In this study,Tremella fuciformis residues as raw material,dietary fibers from tremella were prepared by multiple enzymes.The structure of dietary fibers from tremella was studied by Fourier transform infrared(FTIR),X...In this study,Tremella fuciformis residues as raw material,dietary fibers from tremella were prepared by multiple enzymes.The structure of dietary fibers from tremella was studied by Fourier transform infrared(FTIR),X-ray diffraction analysis(XRD)and scanning electron microscopy(SEM).We analyzed their lipidlowering properties in vitro(water holding,oil holding swelling cholesterol and sodium cholate binding capacitises)and the hypolipidemic effects in mice.The results showed that tremella dietary fibers presented the infrared absorption spectrum characteristics of polysaccharides and the characteristic diffraction peaks of cellulose type I.SEM results indicated that the surface of insoluble dietary fiber(IDF)was porous,while the soluble dietary fiber(SDF)was relatively compact and spongy.IDF exhibited significantly higher water holding,oil holding,and swelling binding capacities than the corresponding SDF.However,SDF exhibited significantly higher viscosity than IDF.The results showed tremella dietary fibers were significant in swelling,water holding and oil holding,cholesterol and bile acids.In vivo experiment results in mice indicated that SDF has the best effect on hyperlipidemia mice than IDF and total dietary fiber(TDF).SDF showed that the total cholesterol(TC),triglyceride(TG)and low density lipoprotein cholesterol(LDL-C)contents dropped by 28.33%,18.65%,and 48.97%,respectively,while high density lipoprotein cholesterol(HDL-C)content increased by 43.80%.Compared with the high-fat control(HCM)group,the arteriosclerosis index(AI)and liver index(LI)of the SDF group mice showed significant differences,indicating that SDF has a good auxiliary effect of lowering blood lipids.The administration of tremella fibers improved the lipid metabolism disorderly situation of hyperlipidemia mice.These results provide a reference for further research and rational development of T.fuciformis.展开更多
The purpose of this work is to describe the structure and hypoglycaemic activity of high-quality Hericium erinaceus dietary fiber(HQ-HDF),and compared with the H.erinaceus dietary fiber(HDF).Scanning electron microsco...The purpose of this work is to describe the structure and hypoglycaemic activity of high-quality Hericium erinaceus dietary fiber(HQ-HDF),and compared with the H.erinaceus dietary fiber(HDF).Scanning electron microscopy and particle size distribution showed that the ultrasonic-microwave assisted enzymatic method significantly changed the surface structure of HQ-HDF and reduced the particle size.The results of high-performance gel chromatography showed that the ultrasonic-microwave assisted enzymatic method made the macromolecular components degrade into small molecular components.HQ-HDF has significantly improved hyperlipidemic activity in vitro.The results showed that HQ-HDF has blood lipid-lowering.The levels of total cholesterol,triglycerides,low-density lipoprotein and high-density lipoprotein in serum of high-fat mice in medium and high dose group were significantly increased.High dose of HQ-HDF significantly decreased the activities of glutamic-pyruvic transaminase and glutamic oxalacetic transaminase,increased the activities of superoxide dismutase and glutathione peroxidase.High dose of HQ-HDF could decreased the content of malonaldehyde.In addition,histopathological observation of mice liver showed that HQ-HDF could improve the lipid metabolism disorder of liver cells.展开更多
基金financially supported by the Key Projects of the National Research and Development Program of China(2018YFD0400204)。
文摘In this study,Tremella fuciformis residues as raw material,dietary fibers from tremella were prepared by multiple enzymes.The structure of dietary fibers from tremella was studied by Fourier transform infrared(FTIR),X-ray diffraction analysis(XRD)and scanning electron microscopy(SEM).We analyzed their lipidlowering properties in vitro(water holding,oil holding swelling cholesterol and sodium cholate binding capacitises)and the hypolipidemic effects in mice.The results showed that tremella dietary fibers presented the infrared absorption spectrum characteristics of polysaccharides and the characteristic diffraction peaks of cellulose type I.SEM results indicated that the surface of insoluble dietary fiber(IDF)was porous,while the soluble dietary fiber(SDF)was relatively compact and spongy.IDF exhibited significantly higher water holding,oil holding,and swelling binding capacities than the corresponding SDF.However,SDF exhibited significantly higher viscosity than IDF.The results showed tremella dietary fibers were significant in swelling,water holding and oil holding,cholesterol and bile acids.In vivo experiment results in mice indicated that SDF has the best effect on hyperlipidemia mice than IDF and total dietary fiber(TDF).SDF showed that the total cholesterol(TC),triglyceride(TG)and low density lipoprotein cholesterol(LDL-C)contents dropped by 28.33%,18.65%,and 48.97%,respectively,while high density lipoprotein cholesterol(HDL-C)content increased by 43.80%.Compared with the high-fat control(HCM)group,the arteriosclerosis index(AI)and liver index(LI)of the SDF group mice showed significant differences,indicating that SDF has a good auxiliary effect of lowering blood lipids.The administration of tremella fibers improved the lipid metabolism disorderly situation of hyperlipidemia mice.These results provide a reference for further research and rational development of T.fuciformis.
基金The study was financially supported by the Key Projects of the National Research and Development Program of China(Grant No.2018YFD0400204).
文摘The purpose of this work is to describe the structure and hypoglycaemic activity of high-quality Hericium erinaceus dietary fiber(HQ-HDF),and compared with the H.erinaceus dietary fiber(HDF).Scanning electron microscopy and particle size distribution showed that the ultrasonic-microwave assisted enzymatic method significantly changed the surface structure of HQ-HDF and reduced the particle size.The results of high-performance gel chromatography showed that the ultrasonic-microwave assisted enzymatic method made the macromolecular components degrade into small molecular components.HQ-HDF has significantly improved hyperlipidemic activity in vitro.The results showed that HQ-HDF has blood lipid-lowering.The levels of total cholesterol,triglycerides,low-density lipoprotein and high-density lipoprotein in serum of high-fat mice in medium and high dose group were significantly increased.High dose of HQ-HDF significantly decreased the activities of glutamic-pyruvic transaminase and glutamic oxalacetic transaminase,increased the activities of superoxide dismutase and glutathione peroxidase.High dose of HQ-HDF could decreased the content of malonaldehyde.In addition,histopathological observation of mice liver showed that HQ-HDF could improve the lipid metabolism disorder of liver cells.