Human Wharton＇s jelly mesenchymal stem cells were isolated from fetal umbilical cord. Cells were cultured in serumfree neural stem cellconditioned medium or neural stem cellconditioned medium supplemented with Dkk1, ...Human Wharton＇s jelly mesenchymal stem cells were isolated from fetal umbilical cord. Cells were cultured in serumfree neural stem cellconditioned medium or neural stem cellconditioned medium supplemented with Dkk1, a Wnt/13 catenin pathway antagonist, and LeftyA, a Nodal signaling pathway antagonist to induce differentiation into retinal progenitor cells. Inverted microscopy showed that after induction, the spindleshaped or fibroblastlike Wharton＇s jelly mesenchymal stem cells changed into bulbous cells with numerous processes. Immunofluorescent cytochemical stain ing and reversetranscription PCR showed positive expression of retinal progenitor cell markers, Pax6 and Rx, as well as weakly downregulated nestin expression. These results demonstrate that Wharton＇s jelly mesenchymal stem cells are capable of differentiating into retinal progenitor cells in vitro.展开更多
Objective: To explore expressions of multidrug resistance gene (MDR) product P170 and peripheral blood MDR1 mRNA in breast cancer tissue and their clinical significances. Methods: Fluorescent quantitative RT-PCR and i...Objective: To explore expressions of multidrug resistance gene (MDR) product P170 and peripheral blood MDR1 mRNA in breast cancer tissue and their clinical significances. Methods: Fluorescent quantitative RT-PCR and immunohis-tochemistry were used to detect peripheral blood MDR1 mRNA before chemotherapy in breast cancer tissue samples from 32 cases with P170 positive (trial group) and 11 cases with P170 negative (control group). Results: There were 14 cases (43.75%) peripheral blood MDR1 mRNA positive and 18 cases (56.25%) negative in 32 cases P170 positive breast cancer pa-tients, while there were 6 cases (54.55%) peripheral blood MDR1 mRNA positive and 5 cases (45.45%) negative in 11 cases P170 negative breast cancer patients; there wasn't a significance difference with peripheral blood MDR1 mRNA express rate in P170 positive (43.75%) group and P170 negative (54.55%) group (χ2 = 0.383, P > 0.05). There were 12 cases (37.50%) Her-2 positive and 20 cases (62.50%) negative in P170 positive group; there were 2 cases (18.18%) Her-2 positive and 9 cases (81.82%) negative in P170 negative group; there wasn't a significance difference with Her-2 positive rate in P170 posi-tive group (37.50%) and P170 negative (18.18%) group (χ2 = 1.391, P > 0.05). There were 13 cases (40.63%) ER positive and 19 cases (59.37%) negative in P170 positive group; there were 7 cases (63.64%) ER positive and 4 cases (36.36%) negative in P170 negative group; there wasn't a significance difference with ER positive rate in P170 positive group (40.63%) and P170 negative (63.64) group (χ2 = 1.742, P > 0.05). There were 20 cases (62.50%) PR positive and 12 cases (37.50%) negative in P170 positive group; there were 4 cases (36.36%) PR positive and 7 cases (63.64%) negative in P170 negative group; there wasn't a significance difference with PR positive rate in P170 positive group (62.50%) and P170 negative (36.36%) group (χ2 = 2.267, P > 0.05). There were 9 cases (28.12%) with lymphaden metastasis and 23 cases (71.88%) without lymphaden metastasis in P170 positive group; there were 5 cases (45.45%) with lymphaden metastasis and 6 cases (54.55%) without lymphaden metastasis in P170 negative group; there wasn't a significance difference with lymphaden metastasis rate in P170 positive group (28.12%) and P170 negative (45.45%) group (χ2 = 1.120, P > 0.05). Conclusion: 1) There were possibly MDR expressing infrequently in carcinoma tissue and peripheral blood before chemotherapy. 2) FQ-RT-PCR for detecting of periph-eral blood MDR1 mRNA is special, sensitive and reliable. It can be used as new molecular biology diagnostic maker dynamic detecting peripheral blood MDR1 mRNA for regulating chemotherapy proposal and elevating chemotherapy effect.展开更多
Integer factorization (IFP), also called prime factorization, is an important problem in number theory, cryptography, and quantum computation. Factoring large integers to attack the RSA cryptosystem is intractable for...Integer factorization (IFP), also called prime factorization, is an important problem in number theory, cryptography, and quantum computation. Factoring large integers to attack the RSA cryptosystem is intractable for powerful supercomputers, let alone classical computers. In 1994, Shor [1]presented an algorithm that potentially enabled a quantum computer to find prime factors in polynomial time.展开更多
Ultra-high-speed, ultra-large-capacity and ultra-long-haul （3U） are the forever pursuit of optical communication. As a new mode of optical communication, 3U transmission can greatly promote next generation optical i...Ultra-high-speed, ultra-large-capacity and ultra-long-haul （3U） are the forever pursuit of optical communication. As a new mode of optical communication, 3U transmission can greatly promote next generation optical internet and broadband mobile communication network development and technological progress, therefore it has become the focus of international high-tech intellectual property competition ground. This paper introduces the scientific problems, key technologies and important achievements in 3U transmission research.展开更多
基金supported by 2010 Com-advanced School Young Diaph Support Project of Heilongjiang Province,China, No. 1155G60
文摘Human Wharton＇s jelly mesenchymal stem cells were isolated from fetal umbilical cord. Cells were cultured in serumfree neural stem cellconditioned medium or neural stem cellconditioned medium supplemented with Dkk1, a Wnt/13 catenin pathway antagonist, and LeftyA, a Nodal signaling pathway antagonist to induce differentiation into retinal progenitor cells. Inverted microscopy showed that after induction, the spindleshaped or fibroblastlike Wharton＇s jelly mesenchymal stem cells changed into bulbous cells with numerous processes. Immunofluorescent cytochemical stain ing and reversetranscription PCR showed positive expression of retinal progenitor cell markers, Pax6 and Rx, as well as weakly downregulated nestin expression. These results demonstrate that Wharton＇s jelly mesenchymal stem cells are capable of differentiating into retinal progenitor cells in vitro.
基金Zibo Science and Technology Development Projects of Science and Technology Bureau (No. 2007032)
文摘Objective: To explore expressions of multidrug resistance gene (MDR) product P170 and peripheral blood MDR1 mRNA in breast cancer tissue and their clinical significances. Methods: Fluorescent quantitative RT-PCR and immunohis-tochemistry were used to detect peripheral blood MDR1 mRNA before chemotherapy in breast cancer tissue samples from 32 cases with P170 positive (trial group) and 11 cases with P170 negative (control group). Results: There were 14 cases (43.75%) peripheral blood MDR1 mRNA positive and 18 cases (56.25%) negative in 32 cases P170 positive breast cancer pa-tients, while there were 6 cases (54.55%) peripheral blood MDR1 mRNA positive and 5 cases (45.45%) negative in 11 cases P170 negative breast cancer patients; there wasn't a significance difference with peripheral blood MDR1 mRNA express rate in P170 positive (43.75%) group and P170 negative (54.55%) group (χ2 = 0.383, P > 0.05). There were 12 cases (37.50%) Her-2 positive and 20 cases (62.50%) negative in P170 positive group; there were 2 cases (18.18%) Her-2 positive and 9 cases (81.82%) negative in P170 negative group; there wasn't a significance difference with Her-2 positive rate in P170 posi-tive group (37.50%) and P170 negative (18.18%) group (χ2 = 1.391, P > 0.05). There were 13 cases (40.63%) ER positive and 19 cases (59.37%) negative in P170 positive group; there were 7 cases (63.64%) ER positive and 4 cases (36.36%) negative in P170 negative group; there wasn't a significance difference with ER positive rate in P170 positive group (40.63%) and P170 negative (63.64) group (χ2 = 1.742, P > 0.05). There were 20 cases (62.50%) PR positive and 12 cases (37.50%) negative in P170 positive group; there were 4 cases (36.36%) PR positive and 7 cases (63.64%) negative in P170 negative group; there wasn't a significance difference with PR positive rate in P170 positive group (62.50%) and P170 negative (36.36%) group (χ2 = 2.267, P > 0.05). There were 9 cases (28.12%) with lymphaden metastasis and 23 cases (71.88%) without lymphaden metastasis in P170 positive group; there were 5 cases (45.45%) with lymphaden metastasis and 6 cases (54.55%) without lymphaden metastasis in P170 negative group; there wasn't a significance difference with lymphaden metastasis rate in P170 positive group (28.12%) and P170 negative (45.45%) group (χ2 = 1.120, P > 0.05). Conclusion: 1) There were possibly MDR expressing infrequently in carcinoma tissue and peripheral blood before chemotherapy. 2) FQ-RT-PCR for detecting of periph-eral blood MDR1 mRNA is special, sensitive and reliable. It can be used as new molecular biology diagnostic maker dynamic detecting peripheral blood MDR1 mRNA for regulating chemotherapy proposal and elevating chemotherapy effect.
文摘Integer factorization (IFP), also called prime factorization, is an important problem in number theory, cryptography, and quantum computation. Factoring large integers to attack the RSA cryptosystem is intractable for powerful supercomputers, let alone classical computers. In 1994, Shor [1]presented an algorithm that potentially enabled a quantum computer to find prime factors in polynomial time.
文摘Ultra-high-speed, ultra-large-capacity and ultra-long-haul （3U） are the forever pursuit of optical communication. As a new mode of optical communication, 3U transmission can greatly promote next generation optical internet and broadband mobile communication network development and technological progress, therefore it has become the focus of international high-tech intellectual property competition ground. This paper introduces the scientific problems, key technologies and important achievements in 3U transmission research.
