Addition of peroxiredoxin 6 (PRDX6) to IVF fertilization medium maintains motility and longevity of human spermatozoa

This study aims to investigate the protective effects of peroxiredoxin 6 on the total motility and progressive motility of human spermatozoa.Semen samples with normal parameters were collected from 23 males and supplemented with different concentrations of peroxiredoxin 6.All the semen samples were measured according to the WHO 5th manual,and the motile spermatozoa were extracted using IVF fertilization medium supplemented with different peroxiredoxin 6 concentrations.Total motility and progressive motility were observed at different time-points of culture at room temperature.After peroxiredoxin 6 supplementation,all groups had a significant increase in total motility and progressive motility compared to the control group.The difference in total motility and progressive motility between the 0 and 10−7 mM groups was observed at 24 and 48 h of culture at room temperature.At 24 h,the total motility increased by 30%in the control group(16.03±11.91 vs.11.51±7.84),and progressive motility increased by 21%(10.53±9.4 vs.8.31±6.04).A similar trend was observed in the 48 h group.In addition,we also found that peroxiredoxin 6 had a well protective effect on sperm kinetic parameters at 10−7 mM.The findings of this study suggest that peroxiredoxin 6 can enhance sperm total motility and progressive motility in IVF fertilization medium.Peroxiredoxin 6 may have potential benefits for sperm preparation in assisted reproductive technology.

Optimization of a three-row air-suction Brassica chinensis precision metering device based on CFD-DEM coupling simulation

This study aimed to optimize a three-row air-suction Brassica chinensis precision metering device to improve the low seeding performance.ANSYS 17.0 Software was used to analyze the effect of different numbers of suction holes and different suction hole structures on the airflow field.It was found that a suction hole number of 60 was beneficial to the flow field stability and a conical hole structure was beneficial to the adsorption of seeds.Box-Behnken design experiments were carried out with negative pressure,rotational speed,and hole diameter as the experimental factors.The optimal parameter combination was achieved when the negative pressure was 3.96 kPa,the rotational speed of the seeding plate was 1.49 rad/s and the hole diameter was 1.10 mm.The qualification rate of inner,middle,and outer rings were 87.580%,90.548%,and 90.117%,respectively,and the miss seeding rate of inner,middle,and outer rings were 10.915%,7.139%,and 5.920%,respectively.

Optimized design of the pneumatic precision seed-metering device for carrots

The small size, light weight, irregular shape, and impurities that characterize seed groups increase clogging tendencies in the traditional seed-metering device thereby making it difficult to achieve high-speed precision seeding. A pneumatic seed-metering device with good seed-filling performance for carrot was designed in this study. By analyzing the movement state of seeds in the device under the theoretical condition, it was concluded that the minimum critical negative pressure value of air chamber was 0.32 kPa, which provided a theoretical basis for simulation and testing. ANSYS 17.0 Software was used to simulate the shape of the seeding plate hole. By comparing the pressure, air flow stability between the suction surface and the plug removal surface of the convection field, it was concluded that the conical hole was optimal. A bench verification test was conducted on the device. The average qualified rate, missing rate, and replaying rate were 81.48%, 4.07%, and 14.45%, respectively, which provided a strong reference for the design of carrot precision seed-metering device.