Objective:To construct a non-replicating vaccinia virus expressing human papillomavirus 16(HPV16) L1, L2E7 proteins as a candidate vaccine for cervical cancer.Methods:Using vaccinia virus vector, we generated a strain...Objective:To construct a non-replicating vaccinia virus expressing human papillomavirus 16(HPV16) L1, L2E7 proteins as a candidate vaccine for cervical cancer.Methods:Using vaccinia virus vector, we generated a strain of non-replicating recombinant vaccinia virus vaccine expressing HPV16 L1, L2E7 proteins by homologous recombination and identified by PCR and Western-bloting.Results:We demonstrated that the L1, L2E7 gene of HPV16 were integrated into vaccinia genosome and could express L1, L2E7 protein stably when infected the CEF using PCR and Western-blot assay.Conclusion:NTVJL1/L2E7 can express L1, L2E7 protein of HPV16 and can be taken as a candidate vaccine for HPV16-associated diseases.展开更多
基金Support by a grant from the National High Technology Research and Development Program, China (No.2002AA216041).
文摘Objective:To construct a non-replicating vaccinia virus expressing human papillomavirus 16(HPV16) L1, L2E7 proteins as a candidate vaccine for cervical cancer.Methods:Using vaccinia virus vector, we generated a strain of non-replicating recombinant vaccinia virus vaccine expressing HPV16 L1, L2E7 proteins by homologous recombination and identified by PCR and Western-bloting.Results:We demonstrated that the L1, L2E7 gene of HPV16 were integrated into vaccinia genosome and could express L1, L2E7 protein stably when infected the CEF using PCR and Western-blot assay.Conclusion:NTVJL1/L2E7 can express L1, L2E7 protein of HPV16 and can be taken as a candidate vaccine for HPV16-associated diseases.
