Field experiments were conducted to study the effects of the controlled release urea (CRU) application on growth yield and nitrogen recovery efficiency of cotton in the main cotton zone of the Yangtze River basin in 2...Field experiments were conducted to study the effects of the controlled release urea (CRU) application on growth yield and nitrogen recovery efficiency of cotton in the main cotton zone of the Yangtze River basin in 2010. Different nitrogen levels were set in order to determine the suitable dosage of CRU on cotton. The special purpose was to provide evidence for the CRU application in cotton fields. The results show that the application of CRU promotes the growth of cotton significantly and enhances the nitrogen supply in the whole period. Compared to the treatment with total N as base fertilizer (UB), the bod, flower, little bolls and total bolls are increased significantly. There are no significant differences in the yield between the CRU treatment and the treatment of controlled release urea and urea combined application (60%CRU + 40%U), but an increase by 12.38%-22.67% compared to the UB treatment, and an increase by 4.49%-7.23% compared to the treatments of total N split application (UD). The nitrogen uptake of CRU treatment was significantly increased by 13.01%-48.32% and 30.27%-13.01% than UB treatment and UD treatment, respectively. The nutrient recovery efficiency of CRU treatment is increased by 16.42-20.59, 5.92-11.29 and 4.22-12.59 percentage points compared to the UB treatment, UD treatment and 60%CRU + 40%U treatment, respectively. In this study, there was a good linearity relationship between the cotton yield and amount of CRU in Wuxue site. The yield of cotton response to amount of CRU could be described by the model of linear plus plateau in Jingzhou site.展开更多
Chalcone isomerase(CHI),catalyzing isomerization of chalcones,is a crucial enzyme in flavonoid biosynthesis.Three CHI genes were isolated from Clivia miniata and designated as CmCHI1,CmCHI2 and CmCHI3,respectively.Mul...Chalcone isomerase(CHI),catalyzing isomerization of chalcones,is a crucial enzyme in flavonoid biosynthesis.Three CHI genes were isolated from Clivia miniata and designated as CmCHI1,CmCHI2 and CmCHI3,respectively.Multiple sequence alignments and phylogenetic analysis showed that CmCHI1 and CmCHI2 were members of type I CHI proteins,whereas CmCHI3 belonged to type IV CHI proteins.Subcellular localization analysis found that all three CmCHIs had diffused distribution in the cytoplasm similar to green fluorescent protein(GFP).Anthocyanin biosynthesis and gene expression analysis demonstrated that CmCHIs were highly expressed in anthocyanin accumulated tissues.To further functionally characterize the role of CmCHIs,an in vitro enzymatic activity assay was carried out using the purified recombinant proteins.Results showed that CmCHI1 and CmCHI2 could completely convert the substrate naringenin chalcone(NC)into the product naringenin(NA),whereas CmCHI3 seemed nonfunctional as no increment of NA was detected.Further genetic transformation of Arabidopsis tt5-1 mutant validated that CmCHI1 and CmCHI2 rather than CmCHI3 could complement the chi deficient phenotypes.In summary,CmCHI1 and CmCHI2 are the real active CHI genes in Clivia miniata.The results not only broaden our knowledge on flavonoid biosynthesis in C.miniata but also lay a new foundation for further flavonoid modification in C.miniata.展开更多
文摘Field experiments were conducted to study the effects of the controlled release urea (CRU) application on growth yield and nitrogen recovery efficiency of cotton in the main cotton zone of the Yangtze River basin in 2010. Different nitrogen levels were set in order to determine the suitable dosage of CRU on cotton. The special purpose was to provide evidence for the CRU application in cotton fields. The results show that the application of CRU promotes the growth of cotton significantly and enhances the nitrogen supply in the whole period. Compared to the treatment with total N as base fertilizer (UB), the bod, flower, little bolls and total bolls are increased significantly. There are no significant differences in the yield between the CRU treatment and the treatment of controlled release urea and urea combined application (60%CRU + 40%U), but an increase by 12.38%-22.67% compared to the UB treatment, and an increase by 4.49%-7.23% compared to the treatments of total N split application (UD). The nitrogen uptake of CRU treatment was significantly increased by 13.01%-48.32% and 30.27%-13.01% than UB treatment and UD treatment, respectively. The nutrient recovery efficiency of CRU treatment is increased by 16.42-20.59, 5.92-11.29 and 4.22-12.59 percentage points compared to the UB treatment, UD treatment and 60%CRU + 40%U treatment, respectively. In this study, there was a good linearity relationship between the cotton yield and amount of CRU in Wuxue site. The yield of cotton response to amount of CRU could be described by the model of linear plus plateau in Jingzhou site.
基金supported by the National Natural Science Foundation of China[31900252,31972445]the Department of Science and Technology of Jilin Province[20190201299JC,20190303095SF,20130604037TC]the Jilin Province Development and Reform Commission[2020C024-5].
文摘Chalcone isomerase(CHI),catalyzing isomerization of chalcones,is a crucial enzyme in flavonoid biosynthesis.Three CHI genes were isolated from Clivia miniata and designated as CmCHI1,CmCHI2 and CmCHI3,respectively.Multiple sequence alignments and phylogenetic analysis showed that CmCHI1 and CmCHI2 were members of type I CHI proteins,whereas CmCHI3 belonged to type IV CHI proteins.Subcellular localization analysis found that all three CmCHIs had diffused distribution in the cytoplasm similar to green fluorescent protein(GFP).Anthocyanin biosynthesis and gene expression analysis demonstrated that CmCHIs were highly expressed in anthocyanin accumulated tissues.To further functionally characterize the role of CmCHIs,an in vitro enzymatic activity assay was carried out using the purified recombinant proteins.Results showed that CmCHI1 and CmCHI2 could completely convert the substrate naringenin chalcone(NC)into the product naringenin(NA),whereas CmCHI3 seemed nonfunctional as no increment of NA was detected.Further genetic transformation of Arabidopsis tt5-1 mutant validated that CmCHI1 and CmCHI2 rather than CmCHI3 could complement the chi deficient phenotypes.In summary,CmCHI1 and CmCHI2 are the real active CHI genes in Clivia miniata.The results not only broaden our knowledge on flavonoid biosynthesis in C.miniata but also lay a new foundation for further flavonoid modification in C.miniata.