Bacterial quorum sensing（QS） molecules are involved in the coordination of certain behaviors such as biofilm formation, virulence and antibiotic resistance. QS molecules（autoinducers） and their corresponding recep...Bacterial quorum sensing（QS） molecules are involved in the coordination of certain behaviors such as biofilm formation, virulence and antibiotic resistance. QS molecules（autoinducers） and their corresponding receptors have been recognized as important therapeutic targets for drug-resistant infections and biofilm-associated infections（BAI）. This study assessed the multiple biological effects of homogentisic acid y-lactone（HgAL）, a furanone derivative. The anti-QS and anti-biofilm effects of HgAL against PAO1 strain were evaluated using CLSM, SEM, HPLC and other biochemical methods The results showed that HgAL could effectively inhibit the production of pyocyanin and extracellular matrix, as well as reduce the adherence ability and biofilm formation of Pseudomonas aeruginosa. Inhibition of virulence is attributed to the suppressive effect of HgAL on biosynthesis of 3-oxo-C12-HSLand C4-HSL（two kinds of QS signaling molecules in P. aeruginosa）. Our results support HgAL as a potential agent for prevention of BAI in the healthcare settings.展开更多
基金supported by the National Natural Science Foundation of China (Nos. 81401512, 41606186)the Scientific Research Fund of Hunan Provincial Education Department (No. 14B157)+1 种基金China Postdoctoral Science Foundation (No. 2016M602419)the Hong Kong Scholars Program (No. XJ2015022)
文摘Bacterial quorum sensing（QS） molecules are involved in the coordination of certain behaviors such as biofilm formation, virulence and antibiotic resistance. QS molecules（autoinducers） and their corresponding receptors have been recognized as important therapeutic targets for drug-resistant infections and biofilm-associated infections（BAI）. This study assessed the multiple biological effects of homogentisic acid y-lactone（HgAL）, a furanone derivative. The anti-QS and anti-biofilm effects of HgAL against PAO1 strain were evaluated using CLSM, SEM, HPLC and other biochemical methods The results showed that HgAL could effectively inhibit the production of pyocyanin and extracellular matrix, as well as reduce the adherence ability and biofilm formation of Pseudomonas aeruginosa. Inhibition of virulence is attributed to the suppressive effect of HgAL on biosynthesis of 3-oxo-C12-HSLand C4-HSL（two kinds of QS signaling molecules in P. aeruginosa）. Our results support HgAL as a potential agent for prevention of BAI in the healthcare settings.
