Component reconstitution-driven photoelectrochemical sensor for sensitive detection of Cu2+ based on advanced CuS/CdS p-n junction

The rational design of robust photoactive material and artful sensing strategy are vital for the construction of an ultrasensitive photoelectrochemical（PEC） sensor. Although great progress has been made in PEC sensing, the resultant detection performances and adoptable sensing strategies are still limited. Herein, through the design of a subtle component reconstitution strategy, an ultrasensitive PEC sensor is developed for the detection of Cu2+ based on advanced Cu S/Cd S nanohybrids（NHs）.This proposed sensor shows superior sensing performances with a low detection limit of 0.1 n M and a wide detection range from0.2 n M to 60 μM due to the formation of p-n junction between Cu S and Cd S and the component transformation of Cd S to CuxS（x=1,2）. Moreover, such PEC sensor also displays goodish results for monitoring the Cu2+released from apoptotic He La cells in vitro. This idea of component reconstitution provides a new paradigm for the design of advanced PEC sensors.

miRNA-let-7i modulates status epilepticus via the TLR4 pathway

Background:Status epilepticus(SE)is a neurological emergency associated with high mortality and morbidity.Many SE episodes cannot be quickly and effectively terminated with current medications.miRNA-Let-7i,a member of the miRNA-Let-7 family,has been found to be associated with a variety of brain pathophysiological and neurological diseases.However,its role in SE remains elusive and requires further clarification.Methods:The expression of miRNA-Let-7i was detected in temporal lobe epilepsy(TLE)patients and SE model rats using the real-time quantitative polymerase chain reaction(RT-qPCR)method.Behavioral assays were performed in pilocarpine-induced SE model,and a whole-cell current clamp technique was employed to examine neuronal excitability.Neuronal apoptosis was evaluated by Nissl staining and terminal deoxynucleotidyl transferase-mediated dUTP end-labeling(TUNEL)assays.Results:The expression of miRNA-Let-7i was significantly reduced in the cortex and hippocampus of SE rats.The miRNA-Let-7i agomir and antagomir effectively regulated the levels of miRNA-Let-7i.In particular,the agomir significantly reduced the degree of SE and prolonged the latent period of SE,whereas the antagomir increased the degree of seizures and shortened the latent period.In addition,the agomir significantly decreased the frequency of action potentials,while the antagomir significantly increased it.Nissl staining and TUNEL assays demonstrated that the agomir increased the survival and decreased the apoptosis,while the antagomir had the opposite effects.In addition,a Toll-like receptor 4(TLR4)inhibitor rescued the effects of antagomir on SE behavior and expression of IL-6 and TNF-α.Similar results on miRNA-Let-7i expression and effects of TLR4 inhibition were found in brain tissues of TLE patients.Conclusions:The miRNA-Let-7i−TLR4 regulatory pathway is involved in SE,which provides insights into the pathogenesis of SE.