Background:Pedunculoside(PE)is a pentacyclic triterpenoid saponin found in the dried barks of the Ilex rotunda Thunb.In traditional Chinese medicine,the juice extracted from various Ilex species is commonly used for t...Background:Pedunculoside(PE)is a pentacyclic triterpenoid saponin found in the dried barks of the Ilex rotunda Thunb.In traditional Chinese medicine,the juice extracted from various Ilex species is commonly used for treating inflammatory diseases.Objective:This study aims to investigate the potential of PE in promoting the healing of oral ulcers in mice and to explore its possible mechanism.Materials and Methods:A mouse model of oral ulcer was established to evaluate the healing promotion effects of PE,and an in-vitro inflammatory cell model was established by using primary fibroblasts isolated from mouse oral mucosal tissue.Gene transcription and protein expression related to cell proliferation,migration,and collagen synthesis were measured.Results:PE showed the highest cure rate of 85.7%for oral ulcers in mice within 7-days treatment.It significantly promoted cell proliferation and collagen secretion in fibroblast in-vitro model.PE also upregulated the gene transcription of Smad3,Col1a1 and Col3a1.Furthermore,PE antagonized the inhibitory effect of WP1066 on STAT3activation,but did not affect cell migration.Conclusion:This study demonstrates that PE has potential therapeutic effects in promoting oral ulcer healing in mice.The improvement was achieved by the increasing collagen synthesis through the upregulation of STAT3 and Smad3 in mucosa fibroblasts.These results provide a preliminary pharmacological work for developing PE as a potential treatment agent for oral ulcers.展开更多
Background:COVID-19 has been impacting on the whole world critically and constantly since late December 2019.Rapidly increasing infections has raised intense worldwide attention.How to model the evolution of COVID-19 ...Background:COVID-19 has been impacting on the whole world critically and constantly since late December 2019.Rapidly increasing infections has raised intense worldwide attention.How to model the evolution of COVID-19 effectively and efficiently is of great significance for prevention and control.Methods:We propose the multi-chain Fudan-CCDC model based on the original single-chain model in[Shao et al.2020]to describe the evolution of COVID-19 in Singapore.Multi-chains can be considered as the superposition of several single chains with different characteristics.We identify the parameters of models by minimizing the penalty function.Results:The numerical simulation results exhibit the multi-chain model performs well on data fitting.Though unsteady the increments are,they could still fall within the range of±30%fluctuation from simulation results.Conclusion:The multi-chain Fudan-CCDC model provides an effective way to early detect the appearance of imported infectors and super spreaders and forecast a second outbreak.It can also explain the data from those countries where the single-chain model shows deviation from the data.展开更多
基金supported by Infinitus(China)Company Ltd.(research grants No.HPG20220930698).
文摘Background:Pedunculoside(PE)is a pentacyclic triterpenoid saponin found in the dried barks of the Ilex rotunda Thunb.In traditional Chinese medicine,the juice extracted from various Ilex species is commonly used for treating inflammatory diseases.Objective:This study aims to investigate the potential of PE in promoting the healing of oral ulcers in mice and to explore its possible mechanism.Materials and Methods:A mouse model of oral ulcer was established to evaluate the healing promotion effects of PE,and an in-vitro inflammatory cell model was established by using primary fibroblasts isolated from mouse oral mucosal tissue.Gene transcription and protein expression related to cell proliferation,migration,and collagen synthesis were measured.Results:PE showed the highest cure rate of 85.7%for oral ulcers in mice within 7-days treatment.It significantly promoted cell proliferation and collagen secretion in fibroblast in-vitro model.PE also upregulated the gene transcription of Smad3,Col1a1 and Col3a1.Furthermore,PE antagonized the inhibitory effect of WP1066 on STAT3activation,but did not affect cell migration.Conclusion:This study demonstrates that PE has potential therapeutic effects in promoting oral ulcer healing in mice.The improvement was achieved by the increasing collagen synthesis through the upregulation of STAT3 and Smad3 in mucosa fibroblasts.These results provide a preliminary pharmacological work for developing PE as a potential treatment agent for oral ulcers.
基金the National Natural Science Foundation of China(No.11671098)partially supported by Shanghai Science and technology research program(No.19JC1420101)+1 种基金J.C.is supported in part by the National Natural Science Foundation of China(No.11971121)Y.Y.is supported by Shanghai Sailing Program(No.20YF1412400).
文摘Background:COVID-19 has been impacting on the whole world critically and constantly since late December 2019.Rapidly increasing infections has raised intense worldwide attention.How to model the evolution of COVID-19 effectively and efficiently is of great significance for prevention and control.Methods:We propose the multi-chain Fudan-CCDC model based on the original single-chain model in[Shao et al.2020]to describe the evolution of COVID-19 in Singapore.Multi-chains can be considered as the superposition of several single chains with different characteristics.We identify the parameters of models by minimizing the penalty function.Results:The numerical simulation results exhibit the multi-chain model performs well on data fitting.Though unsteady the increments are,they could still fall within the range of±30%fluctuation from simulation results.Conclusion:The multi-chain Fudan-CCDC model provides an effective way to early detect the appearance of imported infectors and super spreaders and forecast a second outbreak.It can also explain the data from those countries where the single-chain model shows deviation from the data.
