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阿尔茨海默病患者血清LncRNA MALAT1、LncRNA NEAT1的表达变化及临床意义 被引量:3
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作者 王锋存 赵秀丽 刘军莉 《中国现代医学杂志》 CAS 北大核心 2022年第18期77-82,共6页
目的 分析阿尔茨海默病(AD)患者血清长链非编码RNA(LncRNA)转移相关的肺腺癌转录本1(MALAT1)、LncRNA核旁斑组装转录本1(NEAT1)的表达变化,探讨其是否参与AD炎症反应和认知功能损伤。方法 选取2018年1月—2021年1月青海大学附属医院收治... 目的 分析阿尔茨海默病(AD)患者血清长链非编码RNA(LncRNA)转移相关的肺腺癌转录本1(MALAT1)、LncRNA核旁斑组装转录本1(NEAT1)的表达变化,探讨其是否参与AD炎症反应和认知功能损伤。方法 选取2018年1月—2021年1月青海大学附属医院收治的AD患者93例为AD组,选取同期该院54例体检健康者为对照组。采用实时荧光定量聚合酶链反应检测血清LncRNA MALAT1、LncRNA NEAT1的表达,酶联免疫吸附试验检测血清超敏C反应蛋白(hs-CRP)、白细胞介素-1β(IL-1β)、IL-6、肿瘤坏死因子α(TNF-α)水平,简易精神状态量表(MMSE)评估认知功能。Pearson或Spearman相关性分析AD患者血清LncRNA MALAT1、LncRNA NEAT1表达与病程、MMSE评分、炎症因子的相关性。ROC曲线分析血清炎症指标、LncRNA MALAT1、LncRNA NEAT1表达对AD的诊断价值。结果 AD组血清LncRNA MALAT1相对表达量和MMSE评分低于对照组,LncRNA NEAT1相对表达量高于对照组(P <0.05)。AD组hs-CRP、IL-1β、IL-6、TNF-α水平高于对照组(P <0.05)。Pearson或Spearman相关性分析显示,AD患者血清LncRNA MALAT1表达与MMSE评分呈正相关(r_(s)=0.587,P <0.05),与hs-CRP、IL-1β、IL-6、TNF-α水平均呈负相关(r_(s)=-0.522、-0.601、-0.574和-0.577,均P <0.05),LncRNA NEAT1表达与MMSE评分呈负相关(r_(s)=-0.593,P <0.05),与hs-CRP、IL-1β水平、IL-6、TNF-α均呈正相关(r=0.487、0.588、0.611和0.573,均P <0.05)。ROC曲线显示,血清LncRNA MALAT1诊断AD的AUC为0.915(95%CI:0.858,0.955),最佳临界值为0.98,其对应的敏感性为94.62%(95%CI:87.92,98.22),特异性为81.48%(95%CI:68.61,90.70);血清LncRNA NEAT1诊断AD的AUC为0.858(95%CI:0.791,0.910),最佳临界值为2.37,其对应的敏感性为90.32%(95%CI:82.45,95.52),特异性为72.22%(95%CI:58.44,83.57)。结论 AD患者血清LncRNA MALAT1低表达、LncRNA NEAT1高表达可能参与AD炎症反应和认知功能损伤,可作为AD的诊断标志物。 展开更多
关键词 阿尔茨海默病 长链非编码RNA 转移相关的肺腺癌转录本1 核旁斑组装转录本1 炎症 认知功能
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Palynoflora and climatic dynamics of the Laizhou Bay of Bohai Sea,North China Plain,since the late middle Pleistocene
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作者 Wen-Xia Wang xiu-li zhao +3 位作者 Shou-Jun Li Lei Zhang Xiao-Li Wang Xiang-Yu Zhang 《Journal of Palaeogeography》 SCIE CSCD 2023年第2期278-295,共18页
The coastal plain of Laizhou Bay in Bohai Sea is a transitional area of land-sea interaction.Sediments in this area can bear significant information of sea-level fluctuation,climate change,as well as regional geologic... The coastal plain of Laizhou Bay in Bohai Sea is a transitional area of land-sea interaction.Sediments in this area can bear significant information of sea-level fluctuation,climate change,as well as regional geological setting.Here,in this study,new sporopollen data from three boreholes(GK138,GK111 and GK95)in the coastal plain of Laizhou Bay,Bohai Sea were investigated,and the pollen spectrum since the late middle Pleistocene was established as six sporopollen assemblage zones(Ⅰ-Ⅵ),i.e.,Pinus-Quercus-Artemisia,Gramineae-Artemisia-Chenopodiaceae,Picea-Pinus-QuercusArtemisia,Picea-Pinus-Betula-Gramineae-Artemisia,Picea-Pinus-Cupressaceae-Chenopodiaceae and Pinus-Quercus-Gramineae-Artemisia-Chenopodiaceae.Combining with existing sedimentary successions and detailed radiocarbon dating results of the sediments from the three boreholes,paleovegetation and climatic evolution since the late middle Pleistocene were reconstructed.The findings revealed that climatic changes in this area since the late middle Pleistocene were characterized by alternating cold-arid and warmhumid conditions,which were well correlated with marine isotopic stages(MIS).The present study offers specific insight into the climatic dynamics in the North China Plain since the late middle Pleistocene and provides evidence of a clear link among the palynoflora in the area,the glacial-interglacial period climatedriven sea-level changes,and the marineδ^(18)O records. 展开更多
关键词 Laizhou Bay Late middle Pleistocene Pollen spectrum PALYNOFLORA Climatic dynamics North China Plain
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Revisiting Silica Networks by Small-angle Neutron Scattering and Synchrotron Radiation X-ray Imaging Techniques 被引量:3
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作者 Xin-Wei Kang Dong Liu +6 位作者 Ping Zhang Ming Kang Feng Chen Qing-Xi Yuan xiu-li zhao Ying-Ze Song Li-Xian Song 《Chinese Journal of Polymer Science》 SCIE CAS CSCD 2020年第9期1006-1014,I0008,共10页
The silicone rubber composites present remarkable mechanical properties due to the double network structure constructed with molecular network of matrix and filler network of silica.Nevertheless,the filler network str... The silicone rubber composites present remarkable mechanical properties due to the double network structure constructed with molecular network of matrix and filler network of silica.Nevertheless,the filler network structure and corresponding reinforcement mechanism are still under debate and need to be further probed with the aid of applicative advanced analysis techniques.Herein,small-angle neutron scattering(SANS)and synchrotron radiation X-ray nano-computed tomography(Nano-CT)techniques are employed to explore the evolution of filler networks of fumed,precipitated and sol-gel silica,respectively.Our studying results reveal the formation of filler network constructed by the interconnecting of branched silica aggregates.And the silica with highly associated structure,pertaining to amorphous morphology,small size,and large surface area,presents short distance and effective molecular chain bridge between aggregates,thus forming strong and steady filler networks.This work would provide deep-seated revisiting of filler networks and corresponding reinforcement mechanism and offer guidance for optimizing the mechanical properties of silicone rubber. 展开更多
关键词 Silica networks Reinforcement mechanism SANS Nano-CT
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Early-Middle Permian palynoflora of Shandong Province,eastern North China 被引量:1
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作者 Tian-Tao Yin Shou-Jun Li +1 位作者 Xiang-Yu Zhang xiu-li zhao 《Journal of Palaeogeography》 SCIE CSCD 2020年第4期456-468,共13页
The Permian Taiyuan and Shanxi formations exposed in Shandong Province,eastern North China,contain abundant spores and pollen.In this study,a total of 42 genera and 146 species of spores and pollen from these Permian ... The Permian Taiyuan and Shanxi formations exposed in Shandong Province,eastern North China,contain abundant spores and pollen.In this study,a total of 42 genera and 146 species of spores and pollen from these Permian formations,native to northern China,are identified and related to the three epochs of the Permian Period(Cisuralian,Guadalupian,and Lopingian Epochs)as two assemblages:Assemblage I—the Laevigatosporites-Granulatisporites assemblage,inferred as the Cisuralian(~298.9-272.9 Ma);and,Assemblage II—the Gulisporites-Sinulatisporites assemblage,inferred as the Guadalupian(~272.9-259.1 Ma).Assemblage I represents growing ferns,whereas Assemblage II represents gymnosperms.The assemblage division and analysis indicated that the palaeoclimate of the study area during Early-Middle Permian time was dominated by warm and humid conditions,and later in the Middle Permian changed into moderately dry conditions. 展开更多
关键词 PERMIAN Spores and pollen Vegetation succession Paleoclimate Environmental change Taiyuan Formation Shanxi Formation
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