PCR Detection and Sequence Analysis of Duck Circovirus in Sick Muscovy Ducks

在从福建省的病了的鸭的鸭 circovirus (DuCV ) 感染被调查。有传染 serositis 的 43 只病了的俄国鸭的肝样品在福建省从 12 个鸭农场被收集。基于 DuCV 的出版序列，二份教材为 DuCV 的察觉被设计，四份教材被设计放大盖住 DuCV 的完全的染色体的四重叠碎片。特定的聚合酶链反应产品从积极样品被放大。碎片然后被克隆进 pMD18-T 向量并且定序，并且 DuCV 的 FJ0601 isolate 的完整的长度染色体组的顺序被获得。聚合酶链反应分析证明积极因为 circovirus 从 12 个农场是 79% 和 10 的鸭的比例是积极的。顺序分析证明 DuCV-FJ0601 的完全的染色体是 1988 bp 并且拥有了对包括了茎环结构和议员蛋白质主题的家庭 Circoviridae 普通的特征。相同分析证明 DuCV 的那 FJ0601 isolate 有到所有四个台湾的身份孤立的 97.3%∼97.5% 核苷酸序列(TC1/2002， TC2/2002， TC3/2002， TC4/2002 ) ，到美洲(33753-52 )isolate 的 82.9% 身份和到德国的 82.3% 身份孤立。然而，有 Clustal W 的种系发生的分析证明 DuCV 的那 FJ0601 isolate 在有台湾的一个普通分支上孤立，并且德国和美洲孤立属于另外的分支。

Changes of oncogene DKK2 and SPOCK1 expression in bladder cancer lesions and their correlation with cancer cell proliferation and invasion

Objective: To investigate the changes of oncogene Dickkopf 2 (DKK2) and sparc/osteonectin, cwcv and kazal-like domains proteoglycan 1 (SPOCK1) expression in bladder cancer lesions and their correlation with cancer cell proliferation and invasion. Methods: The bladder cancer lesions and paracancerous lesions surgically removed in our hospital between March 2016 and March 2018 were collected, and kits were used to measure the mRNA expression levels of DKK2, SPOCK1, proliferation genes and invasion genes. Results: DKK2, hyperplasia suppressor gene (HSG), p16, cell adhesion molecule-1 (CADM1), tissue inhibitor of matrix metalloproteinase 2 (TIMP2) and TIMP4 mRNA expression in bladder cancer lesions were significantly lower than those in paracancerous lesions while SPOCK1, β-catenin, CyclinD1, c-myc, Vimentin and matrix metalloproteinase 2 (MMP2) mRNA expression were significantly higher than those in paracancerous lesions;DKK2 was negatively correlated with β-catenin, CyclinD1, c-myc, Vimentin and MMP2, and positively correlated with HSG, p16, CADM1, TIMP2 and TIMP4;SPOCK1 was positively correlated with β-catenin, CyclinD1, c-myc, Vimentin and MMP2, and negatively correlated with HSG, p16, CADM1, TIMP2 and TIMP4. Conclusion: The low expression of oncogene DKK2 and the high expression of SPOCK1 in bladder cancer lesions are related to the changes of proliferation and invasion genes and may be involved in the growth of lesions.