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Method of Spectrophotometric Microanalysis Based on HRP/PET Self-assembly Film 被引量:1
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作者 xiumingjiang zhichunchen shaomingyang hanfenglin xianfulin 《Chinese Chemical Letters》 SCIE CAS CSCD 2004年第5期547-550,共4页
Horseradish peroxidase monolayer was assembled on the surface of PET-CO2 substrate. The reaction kinetics of HRP/PET film and H2O2 in micro reactor was studied using improved spectrophotometer. The relative activity ... Horseradish peroxidase monolayer was assembled on the surface of PET-CO2 substrate. The reaction kinetics of HRP/PET film and H2O2 in micro reactor was studied using improved spectrophotometer. The relative activity of self-assembly HRP/PET film still remains above 80% after storing for 150 days at 4℃. When applied to determination of H2O2 in sample, the recoveries of H2O2 are 96.5%~101.1%. 展开更多
关键词 Electrostatic self-assembly film horseradish peroxidase (HRP) SPECTROPHOTOMETRY atomic force microscope (AFM).
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