Insights into constructing a stable and efficient microbial consortium system

The concept of labor division and multi-module cooperation of microbial consortia offers it promising potentials in various areas,such as the utilization of complex substrates,synthesis of natural compounds with long metabolic pathways and remediation of environmental pollutants within a hostile environment.Consequently,synthetic microbial consortia represent a new frontier for synthetic biology because they can solve more complex problems than monocultures.However,current research on microbial consortia often involves the simple mixing of multiphase systems,where strains are co-cultured sequentially or individually cultured and then mixed-cultured.The instability and low efficiency of microbial consortia systems hindered their practical application.To construct a stable and efficient microbial consortium,it is essential to consider the different growth and metabolic characteristics of strains,the competition for various nutrients as well as the complex carbon,energy and signaling dynamics within the system.In this review,we provide a progressive strategy for constructing a stable and efficient microbial consortium system across three stages:compromised stage(work together),microenvironment-oriented stage(work better),and metabolite delivery-enhanced stage(work best).The detailed methods and points for attention of each stage are summarized,with a highlight on the technical bottleneck and application limitations.Through the integration of interdisciplinary strategies,such as materials science and mathematical models,the goal of building a stable and efficient microbial consortium is constantly advanced.

Production of palmitoleic acid by oleaginous yeast Scheffersomyces segobiensis DSM 27193 using systematic dissolved oxygen regulation strategy

Palmitoleic acid(POA)can be naturally found only in few oil seeds and has significant applications in pharmaceutical industry.Recently,the isolated oleaginous yeast Scheffersomyces segobiensis DSM 27193 was identified with high content of POA in its intracellular lipid(13.80%).In this study,process optimization focused on dissolved oxygen regulation to improve POA production was conducted.Dynamic agitation was found to do significant enhancement on POA-rich lipid production than aeration regulation.Under the best condition of 1000 r·min^(-1)of agitation and 1 vvm(airvolume/culture volume/min)of aeration,no ethanol was detected during the whole fermentation process,while a dry biomass concentration of 44.80 g·L^(-1)with 13.43 g·L^(-1)of lipid and 2.93 g·L^(-1)of POA was achieved.Transcription analysis revealed that the ethanol synthetic pathway was downregulated under the condition of high agitation,while the expression of the key enzymes responsible for lipid and POA accumulation were enhanced.

LaMnO_(3)/Co_(3)O_(4) nanocomposite for enhanced triethylamine sensing properties

LaMnO_(3) modified Co_(3)O_(4) nanocomposites were prepared by simple hydrothermal method co mbined with sol-gel method.The gas sensitivity properties of pure Co_(3)O_(4) and LaMnO_(3)/Co_(3)O_(4) with different composite proportions are compared.It is found that 0.6-LMO/Co_(3)O_(4) sensor has higher sensitivity to triethylamine(TEA)than pure Co_(3)O_(4) sensor,which is improved by 9.27 times.And the working temperature is reduced from 150 to 130℃.Besides,it has excellent gas selectivity and repeatability.The improvement of the gas sensitivity of LaMnO_(3)/Co_(3)O_(4) sensor may be due to the fact that LaMnO_(3) is an effective catalyst,and the catalytic performance perhaps is beneficial to improving the sensing performance.In addition,the formation of p-p heterojunctions may be the key factor to improve the gas sensing performance.This work provides a new Co_(3)O_(4)-based gas sensing material for the detection of TEA.