3D printing of personalized polylactic acid scaffold laden with GelMA/autologous auricle cartilage to promote ear reconstruction

At present,the clinical reconstruction of the auricle usually adopts the strategy of taking autologous costal cartilage.This method has great trauma to patients,poor plasticity and inaccurate shaping.Three-dimensional(3D)printing technology has made a great breakthrough in the clinical application of orthopedic implants.This study explored the combination of 3D printing and tissue engineering to precisely reconstruct the auricle.First,a polylactic acid(PLA)polymer scaffold with a precisely customized patient appearance was fabricated,and then auricle cartilage fragments were loaded into the 3D-printed porous PLA scaffold to promote auricle reconstruction.In vitro,gelatin methacrylamide(GelMA)hydrogels loaded with different sizes of rabbit ear cartilage fragments were studied to assess the regenerative activity of various autologous cartilage fragments.In vivo,rat ear cartilage fragments were placed in an accurately designed porous PLA polymer ear scaffold to promote auricle reconstruction.The results indicated that the chondrocytes in the cartilage fragments could maintain the morphological phenotype in vitro.After three months of implantation observation,it was conducive to promoting the subsequent regeneration of cartilage in vivo.The autologous cartilage fragments combined with 3D printing technology show promising potential in auricle reconstruction.

Elucidating the promotion mechanism of the ternary cooperative heterostructure toward industrial-level urea oxidation catalysis

From the perspective of electronic structure modulation,it is highly desirable to rationally design the active urea oxidation reaction(UOR)catalysts through interface engineering.The binary cooperative heterostructure systems have been shown significant enhancement for catalyzing UOR,but their performance still remains unsatisfactory for industrialization because of the unfavorable intermediate adsorption/desorption and deficient electron transfer channels.In response,taking the ternary cooperative Ni_5P_(4)/NiSe_(2)/Ni_(3)Se_(4) heterostructure as the proof-of-concept paradigm,a catalytic model is rationally put forward to elucidate the UOR promotion mechanism at the molecular level.The rod-like Ni_5P_(4)/NiSe_(2)/Ni_(3)Se_(4) nanoarrays with three-phase heterojunction are experimentally fabricated on Ni foam(named as Ni_5P_(4)/NiSe_(2)/Ni_(3)Se_(4)/NF)via simple two-step processes.The density functional theory calculations disclose that construction of Ni_5P_(4)/NiSe_(2)/Ni_(3)Se_(4) heterostructure model not only induce charge redistribution at the interfacial region for creating innumerable electron transfer channels,but also endow it with a moderate d-band center that could help to build a balance between adsorption and desorption of diverse UOR intermediates.Benefiting from the unique rod-like nanoarrays with large specific surface area and the optimized electronic structure,the well-designed Ni_5P_(4)/NiSe_(2)/Ni_(3)Se_(4)/NF could act as a robust catalyst for driving UOR at industrial-level current densities under tough environments,offering great potential for commercial applications.

Interface engineering of NiSe_(2) nanowrinkles/Ni_(5)P_(4)nanorods for boosting urea oxidation reaction at large current densities

Deliberate modulation of the electronic structure via interface engineering is one of promising perspectives to build advanced catalysts for urea oxidation reaction(UOR)at high current densities.However,it still remains some challenges originating from the intrinsically sluggish UOR dynamics and the high energy barrier for urea adsorption.In response,we report the coupled NiSe_(2)nanowrinkles with Ni_(5)P_(4)nanorods heterogeneous structure onto Ni foam(denoted as NiSe_(2)@Ni_(5)P_(4)/NF)through successive phosphorization and selenization strategy,in which the produced closely contacted interface could provide high-flux electron transfer pathways.Theoretical findings decipher that the fast charge transfer takes place at the interfacial region from Ni_(5)P_(4)to NiSe_(2),which is conducive to optimizing adsorption energy of urea molecules.As expected,the well-designed NiSe_(2)@Ni_(5)P_(4)/NF only requires the low potential of 1.402 V at the current density of 500 mA·cm^(-2).More importantly,a small Tafel slope of 27.6 mV·dec^(-1),a high turnover frequency(TOF)value of 1.037 s^(-1)as well as the prolonged stability of 950 h at the current density of 100 mA·cm^(-2)are also achieved.This study enriches the understanding on the electronic structure modulation via interface engineering and offers bright prospect to design advanced UOR catalysts.

Modal Interactive Feature Encoder for Multimodal Sentiment Analysis

Multimodal Sentiment analysis refers to analyzing emotions in infor-mation carriers containing multiple modalities.To better analyze the features within and between modalities and solve the problem of incomplete multimodal feature fusion,this paper proposes a multimodal sentiment analysis model MIF(Modal Interactive Feature Encoder For Multimodal Sentiment Analysis).First,the global features of three modalities are obtained through unimodal feature extraction networks.Second,the inter-modal interactive feature encoder and the intra-modal interactive feature encoder extract similarity features between modal-ities and intra-modal special features separately.Finally,unimodal special features and the interaction information between modalities are decoded to get the fusion features and predict sentimental polarity results.We conduct extensive experi-ments on three public multimodal datasets,including one in Chinese and two in English.The results show that the performance of our approach is significantly improved compared with benchmark models.

A Cost-Effective Co-precipitation Method for Synthesizing Indium Tin Oxide Nanoparticles without Chlorine Contamination

Indium tin oxide (ITO) nanoparticles with crystallite size of 12.6 nm and specific surface area of 45.7 m 2 ·g-1 were synthesized by co-precipitation method.The indium solution was obtained by dissolving metal indium in HNO3.The tin solution was obtained by dissolving metal tin in HNO3 and followed by stabilizing with citric acid.The free of chlorine ions in the synthesis process brought several advantages:shortening the synthesis time,decreasing the particle agglomeration,decreasing the chlorine content in the ITO nanoparticles and improving the particle sinterability.This is the first time to report the synthesis of ITO nanoparticles free from chlorine contamination without using the expensive metal alkoxides as starting materials.

A sol-gel method to synthesize indium tin oxide nanoparticles

Transparent conductive indium tin oxide (ITO) nanoparticles were synthesized by a novel sol-gel method. Granulated indium and tin were dissolved in HNO3 and partially complexed with citric acid. A sol-gel process was induced when tertiary butyl alcohol was added dropwise to the above solution. ITO nanoparticles with an average crystallite size of 18.5 nm and surface area of 32.6 m2 /g were obtained after the gel was heat-treated at 700 C. The ITO nanoparticles showed good sinterability, the starting sintering temperature decreased sharply to 900 C, and the 1400 C sintered pellet had a density of 98.1% of theoretical density (TD).

The chimeric genes in the hybrid lineage of Carassius auratus cuvieri(♀)×Carassius auratus red var.(♂)

Hybridization can combine the genomes of different strains or species, which leads to changes of genotype and phenotype in the hybrids. In this study, we aimed to investigate the genetic variations of hybrids(WR-F1 and WR-F2) derived from the intraspecific hybridization of white crucian carp(Carassius auratus cuvieri, WCC, ♀) and red crucian carp(Carassius auratus red var., RCC, ♂). Here, we compared the orthologous genes in the liver transcriptomes of hybrids with those of WCC and RCC, and classified the orthologous genes into eight gene patterns within three categories(chimera, mutant, and biparental origin genes).The results revealed 19.04%, 4.17% chimeric genes and 6.90%, 5.05% mutations of orthologous genes in WR-F1 and WR-F2 respectively. Seventeen of twenty-three characterized genes(77%) were confirmed to be the chimeras at the genomic DNA level.The GO classification discovered that some chimeric and mutant genes were related to metabolic process, immune system and developmental process in WR-F1. Our results provide the new evidence that hybridization can combine the parental genomes,leading to changes in the genotype of the resultant hybrids. This is the first report on the formation of chimeric genes from fish intraspecific hybridization, which is potentially interesting from the context of both evolution and the genetic breeding of fish.

Characterization of H3 methylation in regulating oocyte development in cyprinid fish

Histone post-modifications are important epigenetic markers involved in multiple cellular processes via regulation of gene transcription or remodeling of chromatin structure. Oocyte development is a critical process under rigorous control to prevent the generation of aberrant gametes. However, the regulatory mechanism of oocyte early development is not well-understood due to the tiny size and poor distinguishability of the gonad in juvenile stages. Here, two cyprinid hybrid fishes, a sterile allotriploid fish and a gynogenetic hybrid fish with delayed oocyte development, provided research models to investigate the mechanisms involved. We used cytogenetic and molecular methods to confirm the pachytene arrest of oocytes in allotriploid fish and gynogenetic hybrid fish. On the basis of these developmental differences, we screened 21 different histone H3 modifications by ELISA and found that four modifications(H3 K4 me3, H3 K9 me3, H3 K79 me, and H3 K79 me3) differed significantly in the two cyprinid hybrid fishes. Changes in histone methylation at the three residues(H3 K4, K9, K79) were caused by specific methyltransferases and demethylases. Our results provide new insights into the epigenetic regulation of oocyte early development in fish, a process critical for understanding of reproductive biology and with practical applications in the aquacultural breeding industry.

Coupling of NiFe-Based Metal-Organic Framework Nanosheet Arrays with Embedded Fe-Ni_(3)S_(2) Clusters as Efficient Bifunctional Electrocatalysts for Overall Water Splitting

Developing highly efficient,easy-to-make and cost-effective bifunctional electrocatalysts for water splitting with lower cell voltages is crucial to producing massive hydrogen fuel.In response,the coupled hierarchical Ni/Fe-based MOF nanosheet arrays with embedded metal sulfide nanoclusters onto nickel foam skeleton(denoted as Fe-Ni_(3)S_(2)@NiFe-MOF/NF)are fabricated,in which the Fe-Ni_(3)S_(2) clusters could effectively restrain the aggregation of the layer metal-organic frameworks(MOF)nanosheets and adjust the local electronic structures of MOFs nanosheets.Benefiting from the rapid charge transfer and the exposure of abundant active sites,the well-designed Fe-Ni_(3)S_(2)@NiFe-MOF/NF displays excellent oxygen evolution reaction(OER)and hydrogen evolution reaction(HER)performance.More importantly,when equipped in the alkaline water electrolyzer,the Fe-Ni_(3)S_(2)@Ni Fe-MOF/NF enables the system with a mere 1.6 V for achieving the current density of 10 mA cm^(-2).This work offers a paradigm for designing efficient bifunctional HER/OER electrocatalysts based on the hybrid materials of nanostructured metal sulfide and MOF.

Controllable Ni/NiO interface engineering on N-doped carbon spheres for boosted alkaline water-to-hydrogen conversion by urea electrolysis

Interface engineering has gradually attracted substantial research interest in constructing active bifunctional catalysts toward urea electrolysis.The fundamental understanding of the crystallinity transition of the components on both sides of the interface is extremely significant for realizing controllable construction of catalysts through interface engineering,but it still remains a challenge.Herein,the Ni/NiO heterogenous nanoparticles are successfully fabricated on the porous N-doped carbon spheres by a facile hydrothermal and subsequent pyrolysis strategy.And for the first time we show the experimental observation that the Ni/NiO interface can be fine-tuned via simply tailoring the heating rate during pyrolysis process,in which the crystalline/amorphous or crystalline/crystalline Ni/NiO heterostructure is deliberately constructed on the porous N-doped carbon spheres(named as CA-Ni/NiO@NCS or CC-Ni/NiO@NCS,respectively).By taking advantage of the unique porous architecture and the synergistic effect between crystalline Ni and amorphous NiO,the well-designed CA-Ni/NiO@NCS displays more remarkable urea oxidation reaction(UOR)and hydrogen evolution reaction(HER)activity than its crystalline/crystalline counterpart of CC-Ni/NiO@NCS.Particularly,the whole assembled two-electrode electrolytic cell using the elaborate CANi/NiO@NCS both as the anode and cathode can realize the current density of 10 mA·cm^(−2)at a super low voltage of 1.475 V(264 mV less than that of pure water electrolysis),as well as remarkable prolonged stability over 63 h.Besides,the H_(2)evolution driven by an AA battery and a commercial solar cell is also studied to enlighten practical applications for the future.

MCA-TFP Model:A Short-Term Traffic Flow Prediction Model Based on Multi-characteristic Analysis

With the urbanization,urban transportation has become a key factor restricting the development of a city.In a big city,it is important to improve the efficiency of urban transportation.The key to realize short-term traffic flow prediction is to learn its complex spatial correlation,temporal correlation and randomness of traffic flow.In this paper,the convolution neural network(CNN)is proposed to deal with spatial correlation among different regions,considering that the large urban areas leads to a relatively deep Network layer.First three gated recurrent unit(GRU)were used to deal with recent time dependence,daily period dependence and weekly period dependence.Considering that each historical period data to forecast the influence degree of the time period is different,three attention mechanism was taken into GRU.Second,a twolayer full connection network was applied to deal with the randomness of short-term flow combined with additional information such as weather data.Besides,the prediction model was established by combining these three modules.Furthermore,in order to verify the influence of spatial correlation on prediction model,an urban functional area identification model was introduced to identify different functional regions.Finally,the proposed model was validated based on the history of New York City taxi order data and reptiles for weather data.The experimental results show that the prediction precision of our model is obviously superior to the mainstream of the existing prediction methods.

StarIn:An Approach to Predict the Popularity of GitHub Repository

The popularity of repository in GitHub is an important indicator to evaluate its quality.Exploring the trend of popularity is a crucial guideline to study its development potential.Herein,StarIn,a stargazer-influence based approach is proposed to predict the popularity of GitHub repository.Using the followers in GitHub as a basic dataset,stargazer-following based network was established.The indicator,stargazer influence,was measured from three aspects of basic influence,network dynamic influence and network static influence.Experiments was conducted,and the correlation of StarIn was analyzed with the popularity of repository from the perspective of six characteristics.The experimental evaluation provides an interesting approach to predict the popularity of repositories in GitHub from a new perspective.StarIn achieves an excellent performance of predicting the popularity of repositories with a high accurate rate under two different classifiers.

One-step co-doping of ZnO and Zn^(2+)in osteoinductive calcium phosphate ceramics with synergistic antibacterial activity for regenerative repair of infected bone defect

How to endow bone grafts with long-term antibacterial activity and good bone regenerative ability to achieve the regenerative repair of infected bone defects has been the focus of the clinical treatment of osteomyelitis.The present study introduced a novel one-step route to realizing the co-doping of zinc oxide(ZnO)and zinc ion(Zn^(2+))in biphasic calcium phosphate(BCP)ceramics to utilize their synergistic antibacterial.Compared with the conventional BCP ceramics(BCP-Ca),the ZnO/Zn^(2+)co-doping ones(BCP-Zn)possessed strong antibacterial ability on E.coli and S.aureus as well as stimulated the osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs)effectively.The synergistic antibacterial mechanism of ZnO and Zn^(2+)was also investigated.BCP-Zn showed excellent osteoinductivity and angiogenesis at three months postoperatively in the canine intramuscular implantation model.Moreover,BCP-Zn exhibited excellent anti-infective ability and bone regenerative repair compared to BCP-Ca and control groups in the infected bone defect model of rat femur.Collectively,these findings suggest that the simultaneous introduction of ZnO/Zn^(2+)could have immense potential to expand the application of osteoinductive BCP ceramics in the regenerative repair of infected bone defects.