The development of near isogenic lines(NILs) is an important tool for physiological dissection of drought resistance in wheat. To better understand the potential for improving grain yield, a split-plot experiment was ...The development of near isogenic lines(NILs) is an important tool for physiological dissection of drought resistance in wheat. To better understand the potential for improving grain yield, a split-plot experiment was conducted under a mobile rain shelter using NILs of winter wheat with significant differences in the photosynthetic rate: the 908120 line with a high photosynthetic rate and the 908206 line with a low photosynthetic rate. The results indicated that the net photosynthesis rate(Pn), stomatal conductance, and transpiration rate in flag leaves in line 908120 were significantly higher than that in line 908206 under uniform water treatments during the reproductive phase in replicated pooled-culture trials. The maximum quantum yield Fv/Fm value and ribulose-1,5-bisphosphate carboxylase/oxygenase(RuBPCase) activity value were higher in line 908120 than that in line 908206, whereas the intercellular CO_2 and cell membrane permeability in line 908120 were lower than that in line 908206. Higher leaf Pn, transpiration rate, and RuBPCase activity and lower membrane ion leakage rate ensured the robustness of line 908120 during times of irrigation and drought, which contributed to the maintenance of its high grain yield. Drought had a negative effect on these factors, resulting in decreased yield, and the photosynthesis rate of flag leaves markedly affected the yield in NILs of winter wheat. NILs are an important strategy for wheat adaptation to drought stress, but might not be the only mechanism causing the significant grain yield increase. Collectively, the characteristics of line 908120 resulted in a higher grain yield compared with that of line 908206. Further dissection of the drought avoidance mechanisms in wheat, as well as analysis and identification of the genes involved may be necessary.展开更多
We found compound 12N-p-trifluoromethylbenzenesulfonyl matrinane(1)was a potent anti-diabetic agent.Thirty-five tricyclic matrinic derivatives were synthesized and determined for their stimulatory effects on glucose c...We found compound 12N-p-trifluoromethylbenzenesulfonyl matrinane(1)was a potent anti-diabetic agent.Thirty-five tricyclic matrinic derivatives were synthesized and determined for their stimulatory effects on glucose consumption in L6 myotubes,taking 1 as the lead.In high-fat diet(HFD)and STZ induced diabetic mice,9a significantly lowers blood glucose,improves glucose tolerance,and especially alleviates diabetic nephropathy and islet damage.Mechanism study indicates that 9a simultaneously targets mitochondrial complex I to increase AMP/ATP ratio,as well as liver kinase B1(LKB1)and calcium/calmodulindependent protein kinase(Ca MKK),which synergistically activates AMPKαand then stimulates glucose transporter 4(GLUT4)membrane translocation and 2-deoxyglucose(2-DG)uptake to exert anti-diabetic efficacy.Therefore,compound 9a with a novel structure is a promising anti-diabetic candidate with the advantage of multiple-target mechanism,worthy of further investigation.展开更多
基金Supported by National Key Research and Development Program(2017YFD0201702)the Innovation Program of Chinese Academy of Agricultural Sciences(CAAS-XTCX2016019)
文摘The development of near isogenic lines(NILs) is an important tool for physiological dissection of drought resistance in wheat. To better understand the potential for improving grain yield, a split-plot experiment was conducted under a mobile rain shelter using NILs of winter wheat with significant differences in the photosynthetic rate: the 908120 line with a high photosynthetic rate and the 908206 line with a low photosynthetic rate. The results indicated that the net photosynthesis rate(Pn), stomatal conductance, and transpiration rate in flag leaves in line 908120 were significantly higher than that in line 908206 under uniform water treatments during the reproductive phase in replicated pooled-culture trials. The maximum quantum yield Fv/Fm value and ribulose-1,5-bisphosphate carboxylase/oxygenase(RuBPCase) activity value were higher in line 908120 than that in line 908206, whereas the intercellular CO_2 and cell membrane permeability in line 908120 were lower than that in line 908206. Higher leaf Pn, transpiration rate, and RuBPCase activity and lower membrane ion leakage rate ensured the robustness of line 908120 during times of irrigation and drought, which contributed to the maintenance of its high grain yield. Drought had a negative effect on these factors, resulting in decreased yield, and the photosynthesis rate of flag leaves markedly affected the yield in NILs of winter wheat. NILs are an important strategy for wheat adaptation to drought stress, but might not be the only mechanism causing the significant grain yield increase. Collectively, the characteristics of line 908120 resulted in a higher grain yield compared with that of line 908206. Further dissection of the drought avoidance mechanisms in wheat, as well as analysis and identification of the genes involved may be necessary.
基金supported by CAMS Innovation Fund for Medical Sciences(No.2021-12M-1-030)the Natural Science Foundation of Beijing Municipality(No.7202131)Chinese Pharmaceutical Association-Yiling Pharmaceutical Innovation Fund for Biomedicine(No.GL-1-B04-20190397)。
文摘We found compound 12N-p-trifluoromethylbenzenesulfonyl matrinane(1)was a potent anti-diabetic agent.Thirty-five tricyclic matrinic derivatives were synthesized and determined for their stimulatory effects on glucose consumption in L6 myotubes,taking 1 as the lead.In high-fat diet(HFD)and STZ induced diabetic mice,9a significantly lowers blood glucose,improves glucose tolerance,and especially alleviates diabetic nephropathy and islet damage.Mechanism study indicates that 9a simultaneously targets mitochondrial complex I to increase AMP/ATP ratio,as well as liver kinase B1(LKB1)and calcium/calmodulindependent protein kinase(Ca MKK),which synergistically activates AMPKαand then stimulates glucose transporter 4(GLUT4)membrane translocation and 2-deoxyglucose(2-DG)uptake to exert anti-diabetic efficacy.Therefore,compound 9a with a novel structure is a promising anti-diabetic candidate with the advantage of multiple-target mechanism,worthy of further investigation.