A metal-insulator-metal(MIM)-based arc-shaped resonator coupled with a rectangular stub(MARS) structure is proposed. This structure can generate two tunable Fano resonances originating from two different mechanisms. T...A metal-insulator-metal(MIM)-based arc-shaped resonator coupled with a rectangular stub(MARS) structure is proposed. This structure can generate two tunable Fano resonances originating from two different mechanisms. The structure has the advantage of being sensitive to the refractive index, and this feature makes it favorable for application in various microsensors. The relationship between the structural parameters and Fano resonance is researched using the finite element method(FEM) based on the software COMSOL Multiphysics 5.4. The simulation reveals that the sensitivity reaches1900 nm/refractive index unit(RIU), and the figure of merit(FOM) is 23.75.展开更多
Magnetoreception is essential for magnetic orientation in animal migration. The molecular basis for magnetoreception has recently been elucidated in fruitfly as complexes between the magnetic receptor magnetoreceptor(...Magnetoreception is essential for magnetic orientation in animal migration. The molecular basis for magnetoreception has recently been elucidated in fruitfly as complexes between the magnetic receptor magnetoreceptor(Mag R) and its ligand cryptochrome(Cry). Mag R and Cry are present in the animal kingdom. However, it is unknown whether they perform a conserved role in diverse animals. Here we report the identification and expression of zebrafish Mag R and Cry homologs towards understanding their roles in lower vertebrates. A single magr gene and 7 cry genes are present in the zebrafish genome. Zebrafish has four cry1 genes(cry1aa, cry1 ab, cry1 ba and cry1bb) homologous to human CRY1 and a single ortholog of human CRY2 as well as 2 cry-like genes(cry4 and cry5). By RT-PCR, magr exhibited a high level of ubiquitous RNA expression in embryos and adult organs, whereas cry genes displayed differential embryonic and adult expression. Importantly, magr depletion did not produce apparent abnormalities in organogenesis. Taken together, magr and cry2 exist as a single copy gene, whereas cry1 exists as multiple gene duplicates in zebrafish. Our result suggests that magr may play a dispensable role in organogenesis and predicts a possibility to generate magr mutants for analyzing its role in zebrafish.展开更多
HNULP1,a new member of the basic helixloop-helix transcription factors,contains a DUF654 domain in its C-terminus and is highly conserved from Drosophilae,yeast,zebrafish to mouse.The function of this motif,however,is...HNULP1,a new member of the basic helixloop-helix transcription factors,contains a DUF654 domain in its C-terminus and is highly conserved from Drosophilae,yeast,zebrafish to mouse.The function of this motif,however,is currently unknown.In this research,we fused five deletion fragments of the DUF654 domain to the GAL4 DNA-binding domain and then co-transfected with plasmids L8G5-Luc and VP-16.The analysis of the GAL4 luciferase reporter gene indicated that fragments from 228 to 407 amino acids in the DUF654 domain had a strong transcription repression activity.Therefore,this study lays a solid foundation for research on the mechanism of hnulp1 transcriptional regulation and the function of the DUF654 domain.展开更多
基金supported in part by the National Natural Science Foundation of China (Grant Nos. 61875250 and 61975189)the Zhejiang Provincial Natural Science Foundation of China (Grant Nos. LD21F050001 and Y21F040001)+3 种基金the Key Research Project by Department of Water Resources of Zhejiang Province (Grant No. RA2101)the Key Research and Development Project of Zhejiang Province (Grant No. 2021C03019)the Key R&D Projects of Shanxi Province (Grant Nos. 201903D421032 and 01804D131038)the Scientific Research Foundation of Zhejiang University of Water Resources and Electric Power (Grant No. xky2022032)。
文摘A metal-insulator-metal(MIM)-based arc-shaped resonator coupled with a rectangular stub(MARS) structure is proposed. This structure can generate two tunable Fano resonances originating from two different mechanisms. The structure has the advantage of being sensitive to the refractive index, and this feature makes it favorable for application in various microsensors. The relationship between the structural parameters and Fano resonance is researched using the finite element method(FEM) based on the software COMSOL Multiphysics 5.4. The simulation reveals that the sensitivity reaches1900 nm/refractive index unit(RIU), and the figure of merit(FOM) is 23.75.
基金supported by the National Natural Science Foundation of China (31572349, 31272396) to Yuequn Wangthe China Scholarship Council (201406720012) to Xiyang Peng+1 种基金the Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province (2013-448-6)the National Research Foundation of Singapore (NRF-CRP7-2010-03) to Yunhan Hong
文摘Magnetoreception is essential for magnetic orientation in animal migration. The molecular basis for magnetoreception has recently been elucidated in fruitfly as complexes between the magnetic receptor magnetoreceptor(Mag R) and its ligand cryptochrome(Cry). Mag R and Cry are present in the animal kingdom. However, it is unknown whether they perform a conserved role in diverse animals. Here we report the identification and expression of zebrafish Mag R and Cry homologs towards understanding their roles in lower vertebrates. A single magr gene and 7 cry genes are present in the zebrafish genome. Zebrafish has four cry1 genes(cry1aa, cry1 ab, cry1 ba and cry1bb) homologous to human CRY1 and a single ortholog of human CRY2 as well as 2 cry-like genes(cry4 and cry5). By RT-PCR, magr exhibited a high level of ubiquitous RNA expression in embryos and adult organs, whereas cry genes displayed differential embryonic and adult expression. Importantly, magr depletion did not produce apparent abnormalities in organogenesis. Taken together, magr and cry2 exist as a single copy gene, whereas cry1 exists as multiple gene duplicates in zebrafish. Our result suggests that magr may play a dispensable role in organogenesis and predicts a possibility to generate magr mutants for analyzing its role in zebrafish.
基金supported in part by the National Natural Science Foundation of China (Grant Nos.90508004,30470867,30570934,30671054,30671053,30671171,30670274,30671137)Program for Changjiang Scholars and Innovative Research Team in University,China (No.IRT0445)+2 种基金National Basic Research Program of China (No.2005CB522505)New Century Excellent Talents in University (NCET-05-0713)Hunan Provincial Natural Science Foundation of China (No.05J2007,06JJ4120).
文摘HNULP1,a new member of the basic helixloop-helix transcription factors,contains a DUF654 domain in its C-terminus and is highly conserved from Drosophilae,yeast,zebrafish to mouse.The function of this motif,however,is currently unknown.In this research,we fused five deletion fragments of the DUF654 domain to the GAL4 DNA-binding domain and then co-transfected with plasmids L8G5-Luc and VP-16.The analysis of the GAL4 luciferase reporter gene indicated that fragments from 228 to 407 amino acids in the DUF654 domain had a strong transcription repression activity.Therefore,this study lays a solid foundation for research on the mechanism of hnulp1 transcriptional regulation and the function of the DUF654 domain.
