Objective To investigate the old patients with aortic stenosis after transcatheter aortic valve implantation (TAVI) heating conditions,including the incidence of fever, heating time, risk factors, and the recent clini...Objective To investigate the old patients with aortic stenosis after transcatheter aortic valve implantation (TAVI) heating conditions,including the incidence of fever, heating time, risk factors, and the recent clinical outcomes, as TAVI postoperative fever prevention measures,provide clinical basis for the establishment of nursing standard.展开更多
As an important platform for the combination of theory and practice,college students' innovative and entrepreneurial training program has an important impact on improving the comprehensive ability of undergraduate...As an important platform for the combination of theory and practice,college students' innovative and entrepreneurial training program has an important impact on improving the comprehensive ability of undergraduates. The major of aquatic animal medicine is a new sea-related major. The continuous development and transformation and upgrading of aquaculture in the new period have put forward newer and higher requirements for the innovative and practical ability and comprehensive quality of aquatic animal medicine graduates. Taking the innovation and entrepreneurship program of college students majoring in aquatic animal medicine as an example,this paper analyzes the significance of the innovation training program for college students,the cultivation of students' scientific research ability,and the problems existing in the application and operation of the program,in order to provide a reference for the innovative education and talent training for students majoring in aquatic animal medicine.展开更多
Grass carp reovirus(GCRV)is the causative agent of grass carp hemorrhagic disease,which has detrimental effects on the grass carp aquaculture industry.There are four known genotypes of GCRV and strains of different GC...Grass carp reovirus(GCRV)is the causative agent of grass carp hemorrhagic disease,which has detrimental effects on the grass carp aquaculture industry.There are four known genotypes of GCRV and strains of different GCRV genotypes differ greatly.In this study,the diversity of the protein VP6 from different GCRV stains and the effect of genotype GCRV 096 on replication was investigated in CIK cells.Our results showed that the VP6 protein of GCRV 096(genotypeІ)exhibited limited homology to that of GCRV GD108(genotypeШ),with few residues conserved in predicted protein-protein interaction domains.GCRV 096 VP6 protein was expressed and purified and an antiserum against it was characterized.Addition of purified VP6 protein or antiserum to culture media of CIK cells inhibited the replication of GCRV 096 in these cells.In contrast,replication of GCRV GD108(genotypeШ)was not affected in CIK cells under the same condition.Overall,our results indicated that the protein VP6 and VP6 antiserum did not provide cross-protection against GCRV strains and this can be attributed to differences among GCRV genotypes.It will be important to consider multiple GCRV genotypes in the development of effective GCRV vaccines and other therapies against grass carp hemorrhagic disease.In addition,bioinformatics analysis also suggested that the protein VP6 may have a role in the process of GCRV infection.This study lays the foundation for the prevention of grass carp hemorrhagic disease and further detailed studies on the pathogenesis of GCRV.展开更多
The ocean is a crucial area for future economic development.The marine environment has high energy-efficient and ecological requirements for building construction.Meteorological parameters are the key basis for the an...The ocean is a crucial area for future economic development.The marine environment has high energy-efficient and ecological requirements for building construction.Meteorological parameters are the key basis for the analysis and design of building energy efficiency.The lack of meteorological parameters for energy efficiency,particularly hourly data,under oceanic climatic conditions is a universal problem.The appropriate calculation methods of hourly meteorological parameters under oceanic climatic conditions are explored in this study.The impact of the calculation errors of the hourly meteorological parameters on building energy consumption is also analyzed.Three key meteorological parameters are selected:temperature,humidity,and wind speed.Five hourly calculations methods,including linear interpolation,cubic spline interpolation,pieceated three-Hermite interpolation,Akima interpolation,and radial basis function interpolation,are selected to calculate the error of the difference method,with Xiamen,Haikou,and Sanya as the locations of meteorological research.Appropriate interpolation methods are selected for the three parameters,and the seasonal and regional characteristics of the errors of each parameter are compared.Different interpolation methods should be selected for different meteorological parameters in different seasons.The error data of the three parameters of different magnitudes are constructed.A quantitative relationship between the sum of squares due to error of the three meteorological parameters and the rate of change of cooling energy consumption is established.The hourly calculation errors of meteorological parameters have an important impact on the calculation of dynamic energy consumption.The energy consumption differences caused by the errors of different parameters are significant.Obvious regional and seasonal differences also exist.This research strengthens the research foundation of building energy consumption calculation under oceanic climate conditions.展开更多
文摘Objective To investigate the old patients with aortic stenosis after transcatheter aortic valve implantation (TAVI) heating conditions,including the incidence of fever, heating time, risk factors, and the recent clinical outcomes, as TAVI postoperative fever prevention measures,provide clinical basis for the establishment of nursing standard.
基金Supported by Guangdong Higher Education Teaching Reform ProjectGuangdong Ocean University Education and Teaching Reform Project(XJG201806)Guangdong Ocean University Degree and Postgraduate Education Reform Research Project(201709)
文摘As an important platform for the combination of theory and practice,college students' innovative and entrepreneurial training program has an important impact on improving the comprehensive ability of undergraduates. The major of aquatic animal medicine is a new sea-related major. The continuous development and transformation and upgrading of aquaculture in the new period have put forward newer and higher requirements for the innovative and practical ability and comprehensive quality of aquatic animal medicine graduates. Taking the innovation and entrepreneurship program of college students majoring in aquatic animal medicine as an example,this paper analyzes the significance of the innovation training program for college students,the cultivation of students' scientific research ability,and the problems existing in the application and operation of the program,in order to provide a reference for the innovative education and talent training for students majoring in aquatic animal medicine.
基金This study was supported by National Natural Science Foundation of China(No.31602199)the Natural Science Foundation of Guangdong Province in China(No.2015A030313622)+3 种基金the Foundation of Guangdong Ocean University(K15246)the Foundation of Zhanjiang(No.2015A03027)the National 973 Plan Project in China(No.2009CB118704)and the project of Department of Education of Guangdong province in China(No.2012LYM_0075).
文摘Grass carp reovirus(GCRV)is the causative agent of grass carp hemorrhagic disease,which has detrimental effects on the grass carp aquaculture industry.There are four known genotypes of GCRV and strains of different GCRV genotypes differ greatly.In this study,the diversity of the protein VP6 from different GCRV stains and the effect of genotype GCRV 096 on replication was investigated in CIK cells.Our results showed that the VP6 protein of GCRV 096(genotypeІ)exhibited limited homology to that of GCRV GD108(genotypeШ),with few residues conserved in predicted protein-protein interaction domains.GCRV 096 VP6 protein was expressed and purified and an antiserum against it was characterized.Addition of purified VP6 protein or antiserum to culture media of CIK cells inhibited the replication of GCRV 096 in these cells.In contrast,replication of GCRV GD108(genotypeШ)was not affected in CIK cells under the same condition.Overall,our results indicated that the protein VP6 and VP6 antiserum did not provide cross-protection against GCRV strains and this can be attributed to differences among GCRV genotypes.It will be important to consider multiple GCRV genotypes in the development of effective GCRV vaccines and other therapies against grass carp hemorrhagic disease.In addition,bioinformatics analysis also suggested that the protein VP6 may have a role in the process of GCRV infection.This study lays the foundation for the prevention of grass carp hemorrhagic disease and further detailed studies on the pathogenesis of GCRV.
基金the National Key Research and Development Program of China(Grant No.2018YFC0704505)the General Program of National Natural Science Foundation of China(Grant No.51878536).
文摘The ocean is a crucial area for future economic development.The marine environment has high energy-efficient and ecological requirements for building construction.Meteorological parameters are the key basis for the analysis and design of building energy efficiency.The lack of meteorological parameters for energy efficiency,particularly hourly data,under oceanic climatic conditions is a universal problem.The appropriate calculation methods of hourly meteorological parameters under oceanic climatic conditions are explored in this study.The impact of the calculation errors of the hourly meteorological parameters on building energy consumption is also analyzed.Three key meteorological parameters are selected:temperature,humidity,and wind speed.Five hourly calculations methods,including linear interpolation,cubic spline interpolation,pieceated three-Hermite interpolation,Akima interpolation,and radial basis function interpolation,are selected to calculate the error of the difference method,with Xiamen,Haikou,and Sanya as the locations of meteorological research.Appropriate interpolation methods are selected for the three parameters,and the seasonal and regional characteristics of the errors of each parameter are compared.Different interpolation methods should be selected for different meteorological parameters in different seasons.The error data of the three parameters of different magnitudes are constructed.A quantitative relationship between the sum of squares due to error of the three meteorological parameters and the rate of change of cooling energy consumption is established.The hourly calculation errors of meteorological parameters have an important impact on the calculation of dynamic energy consumption.The energy consumption differences caused by the errors of different parameters are significant.Obvious regional and seasonal differences also exist.This research strengthens the research foundation of building energy consumption calculation under oceanic climate conditions.