This study investigated the effects of small interfering RNA （siRNA）-mediated silencing of chemokine receptor 4 （CXCR4） on the invasion capacity of human neuroblastoma cell line SH-SY5Y in vitro. Three siRNAs targ...This study investigated the effects of small interfering RNA （siRNA）-mediated silencing of chemokine receptor 4 （CXCR4） on the invasion capacity of human neuroblastoma cell line SH-SY5Y in vitro. Three siRNAs targeting CXCR4 were chemically synthesized and individually transfected into SH-SY5Y cells. Expression of CXCR4 mRNA and protein was signiifcantly sup-pressed in transfected cells by all three sequence-speciifc siRNAs compared with control groups. Furthermore, the invasion capacity of SH-SY5Y cells was signiifcantly decreased following trans-fection with CXCR4-speciifc siRNA compared with the control groups. These data demonstrate that down-regulation of CXCR4 can inhibit in vitro invasion of neuroblastoma.展开更多
A kinetics model of CO_(2) hydrogenation over iron-nickel catalysts was developed based on the detailed mechanism of alkenes re-adsorption and secondary reaction.The corresponding kinetical experiments are conducted i...A kinetics model of CO_(2) hydrogenation over iron-nickel catalysts was developed based on the detailed mechanism of alkenes re-adsorption and secondary reaction.The corresponding kinetical experiments are conducted in a continuous fixed bed reactor.The effect of reaction conditions on catalyst performance was analyzed according to the results of orthogonal experiments.The results of the experiments show that more methane in products can be obtained with iron-nickel catalysts,the trend of which is consistent with the thermodynamic analysis.However,the content of alkenes in products is equivalent with that of alkanes.This shows that the reaction is controlled by kinetics.In all,the results of the experiments also substantiate that the model can give a good representation of the reaction mechanism of CO_(2) hydrogenation over iron-nickel catalysts.The parameters of this model give a better explanation for the question why the iron-nickel catalysts have a higher selectivity toward alkenes compared with other iron-based catalysts.展开更多
基金supported by the National Natural Science Foundation of China,No.81272986the Natural Science Foundation of Shandong Province,No.ZR2011HZ002
文摘This study investigated the effects of small interfering RNA （siRNA）-mediated silencing of chemokine receptor 4 （CXCR4） on the invasion capacity of human neuroblastoma cell line SH-SY5Y in vitro. Three siRNAs targeting CXCR4 were chemically synthesized and individually transfected into SH-SY5Y cells. Expression of CXCR4 mRNA and protein was signiifcantly sup-pressed in transfected cells by all three sequence-speciifc siRNAs compared with control groups. Furthermore, the invasion capacity of SH-SY5Y cells was signiifcantly decreased following trans-fection with CXCR4-speciifc siRNA compared with the control groups. These data demonstrate that down-regulation of CXCR4 can inhibit in vitro invasion of neuroblastoma.
基金the Key Research Project Plan of Shaanxi Province for the financial support。
文摘A kinetics model of CO_(2) hydrogenation over iron-nickel catalysts was developed based on the detailed mechanism of alkenes re-adsorption and secondary reaction.The corresponding kinetical experiments are conducted in a continuous fixed bed reactor.The effect of reaction conditions on catalyst performance was analyzed according to the results of orthogonal experiments.The results of the experiments show that more methane in products can be obtained with iron-nickel catalysts,the trend of which is consistent with the thermodynamic analysis.However,the content of alkenes in products is equivalent with that of alkanes.This shows that the reaction is controlled by kinetics.In all,the results of the experiments also substantiate that the model can give a good representation of the reaction mechanism of CO_(2) hydrogenation over iron-nickel catalysts.The parameters of this model give a better explanation for the question why the iron-nickel catalysts have a higher selectivity toward alkenes compared with other iron-based catalysts.
