Systems metabolic engineering is a multidisciplinary area that integrates systems biology,synthetic biology and evolutionary engineering.It is an efficient approach for strain improvement and process optimization,and ...Systems metabolic engineering is a multidisciplinary area that integrates systems biology,synthetic biology and evolutionary engineering.It is an efficient approach for strain improvement and process optimization,and has been successfully applied in the microbial production of various chemicals including amino acids.In this review,systems metabolic engineering strategies including pathwayfocused approaches,systems biology-based approaches,evolutionary approaches and their applications in two major amino acid producing microorganisms:Corynebacterium glutamicum and Escherichia coli,are summarized.展开更多
The production of L-tryptophan through che- mical synthesis, direct fermentation, bioconversion and enzymatic conversion has been reported. However, the role of the transport system for the aromatic amino acids in L- ...The production of L-tryptophan through che- mical synthesis, direct fermentation, bioconversion and enzymatic conversion has been reported. However, the role of the transport system for the aromatic amino acids in L- tryptophan producing strains has not been fully explored. In this study, the atop gene of the L-tryptophan producing Escherichia coli TRTH strain was disrupted using Red recombination technology and an atoP mutant E. coli TRTH AaroP was constructed. Fed-batch fermentation of E. coli TRTH △aroP was carried out in 30-L fermentor to investigate the L-tryptophan production. Compared with E. coli TRTH, the atoP mutant was able to maintain a higher growth rate during the exponential phase of the fermentation and the L-tryptophan production increased by 13.3%.展开更多
基金This research was supported by National High Technology Research and Development Program of China(2015AA021003)National Natural Science Foundation of China(31470211)+1 种基金Natural Science Foundation of Tianjin(17JCQNJC09500)Foundation(No.2016IM104)of Key Laboratory of Industrial Fermentation Microbiology of Ministry of Education and Tianjin Key Lab of Industrial Microbiology(Tianjin Universityof Science&Technology).
文摘Systems metabolic engineering is a multidisciplinary area that integrates systems biology,synthetic biology and evolutionary engineering.It is an efficient approach for strain improvement and process optimization,and has been successfully applied in the microbial production of various chemicals including amino acids.In this review,systems metabolic engineering strategies including pathwayfocused approaches,systems biology-based approaches,evolutionary approaches and their applications in two major amino acid producing microorganisms:Corynebacterium glutamicum and Escherichia coli,are summarized.
文摘The production of L-tryptophan through che- mical synthesis, direct fermentation, bioconversion and enzymatic conversion has been reported. However, the role of the transport system for the aromatic amino acids in L- tryptophan producing strains has not been fully explored. In this study, the atop gene of the L-tryptophan producing Escherichia coli TRTH strain was disrupted using Red recombination technology and an atoP mutant E. coli TRTH AaroP was constructed. Fed-batch fermentation of E. coli TRTH △aroP was carried out in 30-L fermentor to investigate the L-tryptophan production. Compared with E. coli TRTH, the atoP mutant was able to maintain a higher growth rate during the exponential phase of the fermentation and the L-tryptophan production increased by 13.3%.
