Fritillariae Cirrhosae Bulbus is a well-known traditional Chine medicinal herb. However, the demand for this herb is leading to over-collection and its decline in the wild. This study aims to investigate the ecologica...Fritillariae Cirrhosae Bulbus is a well-known traditional Chine medicinal herb. However, the demand for this herb is leading to over-collection and its decline in the wild. This study aims to investigate the ecological conditions of Fritillaria cirrhosa under the influence of human disturbance, biotic species interactions and climatic conditions. We established a total of 78 plots at 14 sites of F. cirrhosa in its distribution center, the Hengduan Mountains area. At each site, we estimated the abundance of F. cirrhosa at different distances from roads. The diameter and height of F. cirrhosa fruit and bulbs were measured and compared to underground bulb depth. We then analyzed the effects of environmental conditions and human disturbance on the abundance of F. cirrhosa using variance partitioning. We found that(1)abundance of F. cirrhosa and their underground bulb depth showed a significant linear increase with the distance from the main road;(2) the diameter/height of fruits and the diameter/height of fruits/bulbs showed significantly different responses to the human disturbance;(3) the community associates,climate and spatial conditions can explain 58%, 22% and 27%, respectively, of the variance in the F. cirrhosa abundance. These results highlight the fact that human disturbance and biotic factors have a great influence on the survival of F. cirrhosa, even more than climate conditions.展开更多
Ternary transition metal chalcogenides provide a rich platform to search and study intriguing electronic properties. Using angle-resolved photoemission spectroscopy and ab initio calculation, we investigate the electr...Ternary transition metal chalcogenides provide a rich platform to search and study intriguing electronic properties. Using angle-resolved photoemission spectroscopy and ab initio calculation, we investigate the electronic structure of Cu_(2)TlX_(2)(X = Se, Te), ternary transition metal chalcogenides with quasi-two-dimensional crystal structure. The band dispersions near the Fermi level are mainly contributed by the Te/Se p orbitals. According to our ab-initio calculation, the electronic structure changes from a semiconductor with indirect band gap in Cu_(2)TlSe_(2) to a semimetal in Cu_(2)TlTe_(2), suggesting a band-gap tunability with the composition of Se and Te. By comparing ARPES experimental data with the calculated results, we identify strong modulation of the band structure by spin–orbit coupling in the compounds. Our results provide a ternary platform to study and engineer the electronic properties of transition metal chalcogenides related to large spin–orbit coupling.展开更多
We have investigated th steady-state behavior of a V-shape three-level system submitted to the radiative field and to the relaxation process.The coherence induced by simultaneous two-photon transition leads to a stron...We have investigated th steady-state behavior of a V-shape three-level system submitted to the radiative field and to the relaxation process.The coherence induced by simultaneous two-photon transition leads to a strong decrease of medium absorption.In this case,the peak of transparent spectrum is 7 times higher than that of saturant spectrum.展开更多
Root stem cell niche(SCN)consists of a quiescent center(QC)and surrounding stem cells.Disrupted symplastic communication leads to loss of stemness in the whole SCN.Several SCN regulators were reported to move between ...Root stem cell niche(SCN)consists of a quiescent center(QC)and surrounding stem cells.Disrupted symplastic communication leads to loss of stemness in the whole SCN.Several SCN regulators were reported to move between cells for SCN main-tenance.However,single mutant of these regulators is insu?cient to abolish QC stemness despite the high differentiation rate in surrounding stem cells.To dis-sect the mechanism behind such distinct stemness in SCN,we combined the mis-expression strategy with pWOX5:icals3m system in which QC is symplastically isolated.We found the starch accumulation in QC could be synergistically repressed by WUSCHEL-RE-LATED HOMEOBOX 5(WOX5),SHORT-ROOT(SHR),SCARCROW(SCR),and PLETHORA(PLT).Like PLTs,other core regulators also exhibited dimorphicfunctions by inhibiting differentiation at a higher dose while promoting cell division at a low protein level.Being located in the center of the intersected ex-pression zones,QC cells receive the highest level of core regulators,forming the most robust stemness within SCN.WUSCHEL-RELATED HOMEOBOX 5 was su?cient to activate PLT1/2 expression,contributing to the QC-enriched PLTs.Our results provide ex-perimental evidence supporting the long-standing hypothesis that the combination of spatial ex-pression,synergistic function and dosage effect of core regulators result in spatially distinct stemness in SCN.展开更多
Background Fecal microbiota transplantation(FMT)has been well described in the treatment of pediatric diseases;however,the latest updates regarding its use in children are unclear and the concepts involved need to be ...Background Fecal microbiota transplantation(FMT)has been well described in the treatment of pediatric diseases;however,the latest updates regarding its use in children are unclear and the concepts involved need to be revisited.Data sources We performed advanced searches in the MEDLINE,EMBASE,and Cochrane databases using the keywords“Fecal microbiota transplantation OR Fecal microbiota transfer”in the[Title/Abstract]to identify relevant articles published in English within the last five years.To identify additional studies,reference lists of review articles and included studies were manually searched.Retrieved manuscripts(case reports,reviews,and abstracts)were assessed by the authors.Results Among the articles,studies were based on the mechanism(n=28),sample preparation(n=9),delivery approaches(n=23),safety(n=26),and indications(n=67),including Clostridium difficile infection(CDI)and recurrent C.difficile infection(rCDI;n=21),non-alcoholic fatty liver disease(NAFLD;n=10),irritable bowel syndrome(IBS;n=5),inflammatory bowel disease(IBD;n=15),diabetes(n=5),functional constipation(FC;n=4),and autism spectrum disorder(ASD;n=7).Conclusions Concepts of FMT in pediatric diseases have been updated with respect to underlying mechanisms,methodology,indications,and safety.Evidence-based clinical trials for the use of FMT in pediatric diseases should be introduced to resolve the challenges of dosage,duration,initiation,and the end point of treatment.展开更多
Clostridium autoethanogenum protein(CAP)is a new single-cell protein source originating from inactivated bacteria.An in vitro digestion experiment and an 8-wk growth experiment were conducted to evaluate the molecular...Clostridium autoethanogenum protein(CAP)is a new single-cell protein source originating from inactivated bacteria.An in vitro digestion experiment and an 8-wk growth experiment were conducted to evaluate the molecular weight distribution of the CAP hydrolysate,and the effects of dietary CAP levels on the growth performance,plasma parameters,hepatic and intestinal health,and the diversity of gutadherent microbiota of largemouth bass(Micropterus salmoides).The fish(initial body weight of 47.99±0.01 g)were fed diets where CAP gradually replaced 0%(CAP0),12.5%(CAP12.5),25%(CAP25),37.5%(CAP37.5)and 50%(CAP50)of low-temperature steam dried anchovy fish meal(LTFM)in the diet.Results showed that the content of peptides below 1,000 Da in the CAP hydrolysate(0.56 mg/mL)was higher than that of the LTFM hydrolysate(0.48 mg/mL).Dietary CAP inclusion had no negative effect on growth performance,while whole-body lipid content significantly reduced in the CAP25 and CAP50 groups(P<0.05).The plasma alanine aminotransferase activities and triglyceride concentrations in the CAP inclusion groups were significantly lower than those in the CAP0 group(P<0.05).The plasma aspartate aminotransferase activity was significantly reduced in the CAP37.5 group(P<0.05).The richness and diversity of the gut-adhesive microbiota and the proportion of Clostridium sensu stricto 12 in the CAP50 group were significantly higher than those in the CAP0 group(P<0.05).Dietary CAP inclusion inhibited inflammatory responses by down-regulating the mRNA levels of interleukin 1b(IL1b),IL10 and transforming growth factor b1(P<0.05)in the liver.The mRNA levels of acetyl-CoA carboxylase 1 were significantly down-regulated in the CAP12.5,CAP25 and CAP37.5 groups(P<0.05),while that of fatty acid synthase was significantly down-regulated in the CAP50 group(P<0.05).These results demonstrate that dietary CAP inclusion could improve the hepatic and intestinal health of largemouth bass,and can be helpful to further develop CAP as a functional feed ingredient.展开更多
This study applied in vivo and in vitro methods to investigate the effect of dietary N-carbamoylglutamate(NCG)on lipid metabolism,inflammation and apoptosis related-gene expression in visceral adipose tissue and isola...This study applied in vivo and in vitro methods to investigate the effect of dietary N-carbamoylglutamate(NCG)on lipid metabolism,inflammation and apoptosis related-gene expression in visceral adipose tissue and isolated adipocytes of Japanese seabass(Lateolabrax japonicus).A basal diet and a test diet supplemented with 720 mg/kg NCG were fed to the fish for 10 weeks.During the growth trial,no mortality and no significant differences in growth performance were observed in fish between the 2 groups(P>0.05).Plasma Arg content and mRNA level of argininosuccinate synthetase(ASS)in adipose tissue were significantly increased,which indicated that NCG inclusion promoted endogenous Arg synthesis.Thereafter,the potential effects of NCG treatment on lipid metabolism-related genes expression were studied through in vivo and in vitro methods.In the present study,we successfully established a primary adipocytes culture system and isolated pre-adipocytes in vitro of Japanese seabass for the first time.Both the results in vivo and in vitro showed that NCG treatment decreased the mRNA levels of genes related to adipogenesis(fatty acid synthase,FASN),cholesterol synthesis(3-hydroxy-3-methylglutaryl-CoA reductase,HMGCR)and fat deposition(lipoprotein lipase[LPL]and leptin),which revealed the underlying mechanism of NCG on reducing fat deposition.The results of this study demonstrated that NCG inclusion reduced the expression of inflammatory and apoptosis cytokines markedly in vivo and in vitro.In conclusion,NCG did exert beneficial effects on ameliorating adipo-genesis,inflammation and apoptosis via promoting Arg endogenous synthesis in Japanese seabass.展开更多
基金supported by Yunnan Environmental Protection Special Fund 2013, Grant No. 214203the National Natural Science Foundation of China (NSFC) Grant No. 31560063Key Disciplines (Ecology) Project of Yunnan Education Department
文摘Fritillariae Cirrhosae Bulbus is a well-known traditional Chine medicinal herb. However, the demand for this herb is leading to over-collection and its decline in the wild. This study aims to investigate the ecological conditions of Fritillaria cirrhosa under the influence of human disturbance, biotic species interactions and climatic conditions. We established a total of 78 plots at 14 sites of F. cirrhosa in its distribution center, the Hengduan Mountains area. At each site, we estimated the abundance of F. cirrhosa at different distances from roads. The diameter and height of F. cirrhosa fruit and bulbs were measured and compared to underground bulb depth. We then analyzed the effects of environmental conditions and human disturbance on the abundance of F. cirrhosa using variance partitioning. We found that(1)abundance of F. cirrhosa and their underground bulb depth showed a significant linear increase with the distance from the main road;(2) the diameter/height of fruits and the diameter/height of fruits/bulbs showed significantly different responses to the human disturbance;(3) the community associates,climate and spatial conditions can explain 58%, 22% and 27%, respectively, of the variance in the F. cirrhosa abundance. These results highlight the fact that human disturbance and biotic factors have a great influence on the survival of F. cirrhosa, even more than climate conditions.
基金supported by the National Natural Science Foundation of China (Grant No. 11774190)。
文摘Ternary transition metal chalcogenides provide a rich platform to search and study intriguing electronic properties. Using angle-resolved photoemission spectroscopy and ab initio calculation, we investigate the electronic structure of Cu_(2)TlX_(2)(X = Se, Te), ternary transition metal chalcogenides with quasi-two-dimensional crystal structure. The band dispersions near the Fermi level are mainly contributed by the Te/Se p orbitals. According to our ab-initio calculation, the electronic structure changes from a semiconductor with indirect band gap in Cu_(2)TlSe_(2) to a semimetal in Cu_(2)TlTe_(2), suggesting a band-gap tunability with the composition of Se and Te. By comparing ARPES experimental data with the calculated results, we identify strong modulation of the band structure by spin–orbit coupling in the compounds. Our results provide a ternary platform to study and engineer the electronic properties of transition metal chalcogenides related to large spin–orbit coupling.
基金Supported in part by the National Natural Science Foundation of China.
文摘We have investigated th steady-state behavior of a V-shape three-level system submitted to the radiative field and to the relaxation process.The coherence induced by simultaneous two-photon transition leads to a strong decrease of medium absorption.In this case,the peak of transparent spectrum is 7 times higher than that of saturant spectrum.
基金This work is supported by the National Key Research and Development Program of China(2016YFD0100700)the grant from the National Natural Science Foundation of China(31722006)Key Research Program of Fujian Province(2018NZ0002)to S.W.
文摘Root stem cell niche(SCN)consists of a quiescent center(QC)and surrounding stem cells.Disrupted symplastic communication leads to loss of stemness in the whole SCN.Several SCN regulators were reported to move between cells for SCN main-tenance.However,single mutant of these regulators is insu?cient to abolish QC stemness despite the high differentiation rate in surrounding stem cells.To dis-sect the mechanism behind such distinct stemness in SCN,we combined the mis-expression strategy with pWOX5:icals3m system in which QC is symplastically isolated.We found the starch accumulation in QC could be synergistically repressed by WUSCHEL-RE-LATED HOMEOBOX 5(WOX5),SHORT-ROOT(SHR),SCARCROW(SCR),and PLETHORA(PLT).Like PLTs,other core regulators also exhibited dimorphicfunctions by inhibiting differentiation at a higher dose while promoting cell division at a low protein level.Being located in the center of the intersected ex-pression zones,QC cells receive the highest level of core regulators,forming the most robust stemness within SCN.WUSCHEL-RELATED HOMEOBOX 5 was su?cient to activate PLT1/2 expression,contributing to the QC-enriched PLTs.Our results provide ex-perimental evidence supporting the long-standing hypothesis that the combination of spatial ex-pression,synergistic function and dosage effect of core regulators result in spatially distinct stemness in SCN.
基金This project was funded by the National Natural Science Foundation of China(30700917,81570465)Minsheng Foundation of Joint Research Project of Liaoning Province(2021JH2/10300129).
文摘Background Fecal microbiota transplantation(FMT)has been well described in the treatment of pediatric diseases;however,the latest updates regarding its use in children are unclear and the concepts involved need to be revisited.Data sources We performed advanced searches in the MEDLINE,EMBASE,and Cochrane databases using the keywords“Fecal microbiota transplantation OR Fecal microbiota transfer”in the[Title/Abstract]to identify relevant articles published in English within the last five years.To identify additional studies,reference lists of review articles and included studies were manually searched.Retrieved manuscripts(case reports,reviews,and abstracts)were assessed by the authors.Results Among the articles,studies were based on the mechanism(n=28),sample preparation(n=9),delivery approaches(n=23),safety(n=26),and indications(n=67),including Clostridium difficile infection(CDI)and recurrent C.difficile infection(rCDI;n=21),non-alcoholic fatty liver disease(NAFLD;n=10),irritable bowel syndrome(IBS;n=5),inflammatory bowel disease(IBD;n=15),diabetes(n=5),functional constipation(FC;n=4),and autism spectrum disorder(ASD;n=7).Conclusions Concepts of FMT in pediatric diseases have been updated with respect to underlying mechanisms,methodology,indications,and safety.Evidence-based clinical trials for the use of FMT in pediatric diseases should be introduced to resolve the challenges of dosage,duration,initiation,and the end point of treatment.
基金the National Key R&D Program of China(2019YFD0900200 and 2018YFD0900400)the National Natural Science Foundation of China(32172981 and 31902382)+1 种基金the Agricultural Science and Technology Innovation Programof CAAS in China(CAAS-ASTIP-2017-FRI-08)China Postdoctoral Science Foundation(2021M703544).
文摘Clostridium autoethanogenum protein(CAP)is a new single-cell protein source originating from inactivated bacteria.An in vitro digestion experiment and an 8-wk growth experiment were conducted to evaluate the molecular weight distribution of the CAP hydrolysate,and the effects of dietary CAP levels on the growth performance,plasma parameters,hepatic and intestinal health,and the diversity of gutadherent microbiota of largemouth bass(Micropterus salmoides).The fish(initial body weight of 47.99±0.01 g)were fed diets where CAP gradually replaced 0%(CAP0),12.5%(CAP12.5),25%(CAP25),37.5%(CAP37.5)and 50%(CAP50)of low-temperature steam dried anchovy fish meal(LTFM)in the diet.Results showed that the content of peptides below 1,000 Da in the CAP hydrolysate(0.56 mg/mL)was higher than that of the LTFM hydrolysate(0.48 mg/mL).Dietary CAP inclusion had no negative effect on growth performance,while whole-body lipid content significantly reduced in the CAP25 and CAP50 groups(P<0.05).The plasma alanine aminotransferase activities and triglyceride concentrations in the CAP inclusion groups were significantly lower than those in the CAP0 group(P<0.05).The plasma aspartate aminotransferase activity was significantly reduced in the CAP37.5 group(P<0.05).The richness and diversity of the gut-adhesive microbiota and the proportion of Clostridium sensu stricto 12 in the CAP50 group were significantly higher than those in the CAP0 group(P<0.05).Dietary CAP inclusion inhibited inflammatory responses by down-regulating the mRNA levels of interleukin 1b(IL1b),IL10 and transforming growth factor b1(P<0.05)in the liver.The mRNA levels of acetyl-CoA carboxylase 1 were significantly down-regulated in the CAP12.5,CAP25 and CAP37.5 groups(P<0.05),while that of fatty acid synthase was significantly down-regulated in the CAP50 group(P<0.05).These results demonstrate that dietary CAP inclusion could improve the hepatic and intestinal health of largemouth bass,and can be helpful to further develop CAP as a functional feed ingredient.
基金supported by the National Key R&D Program of China(2019YFD0900200,2018YFD0900400)the Agricultural Science and Technology Innovation Program(CAAS-ASTIP-2017-FRI-08)+2 种基金Beijing Technology System for Sturgeon and Salmonids(BAIC08-2021)National Natural Science Foundation of China(31902382)Natural Science Foundation of Beijing(6204047)
文摘This study applied in vivo and in vitro methods to investigate the effect of dietary N-carbamoylglutamate(NCG)on lipid metabolism,inflammation and apoptosis related-gene expression in visceral adipose tissue and isolated adipocytes of Japanese seabass(Lateolabrax japonicus).A basal diet and a test diet supplemented with 720 mg/kg NCG were fed to the fish for 10 weeks.During the growth trial,no mortality and no significant differences in growth performance were observed in fish between the 2 groups(P>0.05).Plasma Arg content and mRNA level of argininosuccinate synthetase(ASS)in adipose tissue were significantly increased,which indicated that NCG inclusion promoted endogenous Arg synthesis.Thereafter,the potential effects of NCG treatment on lipid metabolism-related genes expression were studied through in vivo and in vitro methods.In the present study,we successfully established a primary adipocytes culture system and isolated pre-adipocytes in vitro of Japanese seabass for the first time.Both the results in vivo and in vitro showed that NCG treatment decreased the mRNA levels of genes related to adipogenesis(fatty acid synthase,FASN),cholesterol synthesis(3-hydroxy-3-methylglutaryl-CoA reductase,HMGCR)and fat deposition(lipoprotein lipase[LPL]and leptin),which revealed the underlying mechanism of NCG on reducing fat deposition.The results of this study demonstrated that NCG inclusion reduced the expression of inflammatory and apoptosis cytokines markedly in vivo and in vitro.In conclusion,NCG did exert beneficial effects on ameliorating adipo-genesis,inflammation and apoptosis via promoting Arg endogenous synthesis in Japanese seabass.