Displacement damage effects on the charge-coupled device(CCD)induced by neutrons at the back-streaming white neutron source(Back-n)in the China Spallation Neutron Source(CSNS)are analyzed according to an online irradi...Displacement damage effects on the charge-coupled device(CCD)induced by neutrons at the back-streaming white neutron source(Back-n)in the China Spallation Neutron Source(CSNS)are analyzed according to an online irradiation experiment.The hot pixels,random telegraph signal(RTS),mean dark signal,dark current and dark signal non-uniformity(DSNU)induced by Back-n are presented.The dark current is calculated according to the mean dark signal at various integration times.The single-particle displacement damage and transient response are also observed based on the online measurement data.The trends of hot pixels,mean dark signal,DSNU and RTS degradation are related to the integration time and irradiation fluence.The mean dark signal,dark current and DSNU2 are nearly linear with neutron irradiation fluence when nearly all the pixels do not reach saturation.In addition,the mechanisms of the displacement damage effects on the CCD are demonstrated by combining the experimental results and technology computer-aided design(TCAD)simulation.Radiation-induced traps in the space charge region of the CCD will act as generation/recombination centers of electron-hole pairs,leading to an increase in the dark signal.展开更多
Objective:The aim of this work was to measure the antibacterial activity(against Escherichia coli and Staphylococcus aureus[S.aureus])of the ethyl acetate part of Abrus cantoniensis and assess their potential as medic...Objective:The aim of this work was to measure the antibacterial activity(against Escherichia coli and Staphylococcus aureus[S.aureus])of the ethyl acetate part of Abrus cantoniensis and assess their potential as medicines.Methods:The experiment was divided into four groups:negative control group[with Mueller-Hinton broth(MHB)],positive control group(with 75%ethanol),blank group(with MHB)and test group(with the ethyl acetate part of Abrus cantoniensis).The antibacterial activities of the extracts were evaluated by the Oxford cup assay and minimum inhibitory concentration(MIC).Time-kill curve experiments,scanning electron microscopy,the content of DNA,RNA and protein were used to study the antibacterial mechanism of the ethyl acetate extract part on the growth and viability of S.aureus.The study procedures were approved by the Animal Care and Use Committee of Xi’an Jiaotong University(approval No.XJTULAC2016-412)on January 22,2016.Results:The ethyl acetate part of Abrus cantoniensis extract exhibited the highest inhibitory activity against the growth of S.aureus with an inhibition zone diameter of 16.4mm and MIC value of 0.5mg/mL.The general activity range of the ethyl acetate part,determined using a time-killing curve,was found to be 0.5mg/mL to 40mg/mL(MIC to 80MIC).Changes in the scanning electron microscopy images and of DNA,RNA and proteins of S.aureus indicated possible mechanisms of the inhibitory activity of the ethyl acetate part.Conclusion:The ethyl acetate part of Abrus cantoniensis damaged bacterial cell structures,which results in protoplasm leakage,and eventually bacterial cell death.展开更多
Single-domain antibodies have the characteristics of small molecular weight,strong tissue penetration,and high affinity,and are widely used to construct molecular probes for disease diagnosis and treatment.This articl...Single-domain antibodies have the characteristics of small molecular weight,strong tissue penetration,and high affinity,and are widely used to construct molecular probes for disease diagnosis and treatment.This article reviews molecular imaging studies including positron emission tomography(PET),single-photon emission computed tomography/computed tomography(CT),PET/CT,and fluorescent imaging of molecular probes composed of single-domain antibodies against eight esophageal squamous cell carcinoma biological targets.These 8 targets are highly expressed on the membrane of esophageal squamous cell carcinoma cells and include epidermal growth factor receptor,human epidermal growth factor receptor 2,human epidermal growth factor receptor 3,hepatocyte growth factor receptor,vascular endothelial growth factor receptor 2,chemokine receptor 4,chemokine receptor 7,and carcinoembryonic antigen.The current problems and solutions are also discussed to provide a reference for future design of molecular imaging probes targeting esophageal squamous cell carcinoma.展开更多
Objective::In order to study the important role and molecular mechanism of Brevinin-2 family antimicrobial peptide Brevinin-2ISb in methicillin-resistant Staphylococcus aureus(MRSA)infection of Caenorhabditis(C.)elega...Objective::In order to study the important role and molecular mechanism of Brevinin-2 family antimicrobial peptide Brevinin-2ISb in methicillin-resistant Staphylococcus aureus(MRSA)infection of Caenorhabditis(C.)elegans,and to find the optimal therapeutic concentration of Brevinin-2ISb.Methods::By using a C.elegans model and MRSA infection modelto study the therapeutic effect of different concentrations of Brevinin-2ISb on C.elegans.Real-time PCR was used for investigating the effect of Brevinin-2ISb on the downstream gene expression of DAF-2/DAF-16 innate immune pathway and the major virulence factor gene expression of MRSA.With protein activity tests to study the inhibitory effect of Brevinin-2ISb on MRSA virulence factor protein activity.Finally,laser confocal imaging was carried out to observe real-time expression and distribution of downstream antimicrobial proteins to further prove the effect of Brevinin-2ISb on the activation of DAF-2/DAF-16 pathway by in vivo imaging.All animal study procedures were approved by the Academic Committee at Xidian University and Xi’an Jiaotong University Animal Care and Use Committee,China(approval No.JGC201207)on July 15,2017.Results::Host immunity was largely enhanced by Brevinin-2ISb,and the expression of staphylococcal enterotoxin genes,as well as virulence factors,was suppressed by Brevinin-2ISb.Indeed,the expression of many C.elegans innate immune genes,including lys-7,spp-1,K05D8.5 and C29F3.7,was induced by Brevinin-2ISb.In particular,robust,sustained expression of the antibacterial gene lys-7 was observed after Brevinin-2ISb treatment,resulting in increased protein levels.These effects correlated with a reduction in the MRSA-mediated death of the C.elegans host.Low concentrations of Brevinin-2ISb exhibited very low hemolytic activity,and may play a positive role in host innate immunity.Specifically,activation of the DAF-2/DAF-16 pathway appears to be essential for immune activation in C.elegans treated with Brevinin-2ISb.Based on the evolutionary conservation of innate immune pathways,our results suggest that Brevinin-2ISb not only has strong antibacterial activity,but may also enhance the innate immune response in humans.This study demonstrates that Brevinin-2ISb-related peptides are potential candidates for the development of novel anti-inflammatory or anti-microbial drugs.Conclusion::Antimicrobial peptide Brevinin-2ISb effectively inhibits MRSA at low concentration.This antimicrobial peptide can prolong the life of MRSA-infected C.elegans,has very low hemolytic activity and inhibits the activity and expression of various MRSA virulence factors.More importantly,Brevinin-2ISb activated the expression of antimicrobial genes downstream of DAF-2/DAF-16,which enhanced the MRSA resistance of C.elegans.This peptide could be used as the basis for developing new drugs to replace antibiotics.展开更多
基金Project supported by the Foundation of State Key Laboratory of China(Grant Nos.SKLIPR1903Z,1803)the National Natural Science Foundation of China(Grant Nos.U2167208 and 11875223).
文摘Displacement damage effects on the charge-coupled device(CCD)induced by neutrons at the back-streaming white neutron source(Back-n)in the China Spallation Neutron Source(CSNS)are analyzed according to an online irradiation experiment.The hot pixels,random telegraph signal(RTS),mean dark signal,dark current and dark signal non-uniformity(DSNU)induced by Back-n are presented.The dark current is calculated according to the mean dark signal at various integration times.The single-particle displacement damage and transient response are also observed based on the online measurement data.The trends of hot pixels,mean dark signal,DSNU and RTS degradation are related to the integration time and irradiation fluence.The mean dark signal,dark current and DSNU2 are nearly linear with neutron irradiation fluence when nearly all the pixels do not reach saturation.In addition,the mechanisms of the displacement damage effects on the CCD are demonstrated by combining the experimental results and technology computer-aided design(TCAD)simulation.Radiation-induced traps in the space charge region of the CCD will act as generation/recombination centers of electron-hole pairs,leading to an increase in the dark signal.
基金the National Key Research&Development Program of China,No.2018YFC0910601(to XX)the National Natural Science Foundation of China,Nos.81701853(to QZ)and 62007026(to HX)the Natural Science Basic Research Plan in Shaanxi Province of China,Nos.2019JQ-519(to XX)and 2019JQ-201(to HX).
文摘Objective:The aim of this work was to measure the antibacterial activity(against Escherichia coli and Staphylococcus aureus[S.aureus])of the ethyl acetate part of Abrus cantoniensis and assess their potential as medicines.Methods:The experiment was divided into four groups:negative control group[with Mueller-Hinton broth(MHB)],positive control group(with 75%ethanol),blank group(with MHB)and test group(with the ethyl acetate part of Abrus cantoniensis).The antibacterial activities of the extracts were evaluated by the Oxford cup assay and minimum inhibitory concentration(MIC).Time-kill curve experiments,scanning electron microscopy,the content of DNA,RNA and protein were used to study the antibacterial mechanism of the ethyl acetate extract part on the growth and viability of S.aureus.The study procedures were approved by the Animal Care and Use Committee of Xi’an Jiaotong University(approval No.XJTULAC2016-412)on January 22,2016.Results:The ethyl acetate part of Abrus cantoniensis extract exhibited the highest inhibitory activity against the growth of S.aureus with an inhibition zone diameter of 16.4mm and MIC value of 0.5mg/mL.The general activity range of the ethyl acetate part,determined using a time-killing curve,was found to be 0.5mg/mL to 40mg/mL(MIC to 80MIC).Changes in the scanning electron microscopy images and of DNA,RNA and proteins of S.aureus indicated possible mechanisms of the inhibitory activity of the ethyl acetate part.Conclusion:The ethyl acetate part of Abrus cantoniensis damaged bacterial cell structures,which results in protoplasm leakage,and eventually bacterial cell death.
基金supported,in part,by the National Key Research&Development Program of China(No.2018YFC0910600)the National Natural Science Foundation of China(Nos.81871397,81701853,81627807)+1 种基金the Natural Science Basic Research Plan in Shaanxi Province of China(Nos.2019JQ-519,2019JQ-201,2019JQ-045)the Fundamental Research Funds for the Central Universities(No.JB191209).
文摘Single-domain antibodies have the characteristics of small molecular weight,strong tissue penetration,and high affinity,and are widely used to construct molecular probes for disease diagnosis and treatment.This article reviews molecular imaging studies including positron emission tomography(PET),single-photon emission computed tomography/computed tomography(CT),PET/CT,and fluorescent imaging of molecular probes composed of single-domain antibodies against eight esophageal squamous cell carcinoma biological targets.These 8 targets are highly expressed on the membrane of esophageal squamous cell carcinoma cells and include epidermal growth factor receptor,human epidermal growth factor receptor 2,human epidermal growth factor receptor 3,hepatocyte growth factor receptor,vascular endothelial growth factor receptor 2,chemokine receptor 4,chemokine receptor 7,and carcinoembryonic antigen.The current problems and solutions are also discussed to provide a reference for future design of molecular imaging probes targeting esophageal squamous cell carcinoma.
基金supported in part by the National Key R&D Program of China(No.2018YFC0910600)the National Natural Science Foundation of China(Nos.62007026,81627807,11727813,81871397,81701853,91859109)+3 种基金the Fok Ying-Tong Education Foundation of China(No.161104)the Program for the Young Top-notch Talent of Shaanxi Province,the Research Fund for Young Star of Science and Technology in Shaanxi Province of China(No.2018KJXX-018)the Science and Technology Projects of Xi’an,China(No.201809170CX11JC12)the Natural Science Basic Research Plan in Shaanxi Province of China(No.2019JQ-201).
文摘Objective::In order to study the important role and molecular mechanism of Brevinin-2 family antimicrobial peptide Brevinin-2ISb in methicillin-resistant Staphylococcus aureus(MRSA)infection of Caenorhabditis(C.)elegans,and to find the optimal therapeutic concentration of Brevinin-2ISb.Methods::By using a C.elegans model and MRSA infection modelto study the therapeutic effect of different concentrations of Brevinin-2ISb on C.elegans.Real-time PCR was used for investigating the effect of Brevinin-2ISb on the downstream gene expression of DAF-2/DAF-16 innate immune pathway and the major virulence factor gene expression of MRSA.With protein activity tests to study the inhibitory effect of Brevinin-2ISb on MRSA virulence factor protein activity.Finally,laser confocal imaging was carried out to observe real-time expression and distribution of downstream antimicrobial proteins to further prove the effect of Brevinin-2ISb on the activation of DAF-2/DAF-16 pathway by in vivo imaging.All animal study procedures were approved by the Academic Committee at Xidian University and Xi’an Jiaotong University Animal Care and Use Committee,China(approval No.JGC201207)on July 15,2017.Results::Host immunity was largely enhanced by Brevinin-2ISb,and the expression of staphylococcal enterotoxin genes,as well as virulence factors,was suppressed by Brevinin-2ISb.Indeed,the expression of many C.elegans innate immune genes,including lys-7,spp-1,K05D8.5 and C29F3.7,was induced by Brevinin-2ISb.In particular,robust,sustained expression of the antibacterial gene lys-7 was observed after Brevinin-2ISb treatment,resulting in increased protein levels.These effects correlated with a reduction in the MRSA-mediated death of the C.elegans host.Low concentrations of Brevinin-2ISb exhibited very low hemolytic activity,and may play a positive role in host innate immunity.Specifically,activation of the DAF-2/DAF-16 pathway appears to be essential for immune activation in C.elegans treated with Brevinin-2ISb.Based on the evolutionary conservation of innate immune pathways,our results suggest that Brevinin-2ISb not only has strong antibacterial activity,but may also enhance the innate immune response in humans.This study demonstrates that Brevinin-2ISb-related peptides are potential candidates for the development of novel anti-inflammatory or anti-microbial drugs.Conclusion::Antimicrobial peptide Brevinin-2ISb effectively inhibits MRSA at low concentration.This antimicrobial peptide can prolong the life of MRSA-infected C.elegans,has very low hemolytic activity and inhibits the activity and expression of various MRSA virulence factors.More importantly,Brevinin-2ISb activated the expression of antimicrobial genes downstream of DAF-2/DAF-16,which enhanced the MRSA resistance of C.elegans.This peptide could be used as the basis for developing new drugs to replace antibiotics.