目的探索倒睫电解器用于去除白发的可行性,探讨电解联合外科移植治疗不同程度白癜风白发的临床应用价值。方法对健康志愿者头皮及眉毛受试区在不同电流下进行脱毛,观察平均脱毛时长、单次脱毛成功率,明确不同部位脱毛的最适电流值,并观...目的探索倒睫电解器用于去除白发的可行性,探讨电解联合外科移植治疗不同程度白癜风白发的临床应用价值。方法对健康志愿者头皮及眉毛受试区在不同电流下进行脱毛,观察平均脱毛时长、单次脱毛成功率,明确不同部位脱毛的最适电流值,并观察电解后毛发镜及组织病理学改变;分别采用单独电解、单独毛囊单位提取术(follicular unit extraction,FUE),和电解联合外科移植法(自体表皮移植术/FUE)治疗稳定期白癜风白发,随访3个月,评价疗效、术后恢复时间及不良反应。结果健康志愿者的头部黑发和白发在550 m A电流下的平均脱毛时长分别为(278.00±14.8)s和(373.00±8.19)s,均高于600 m A(248.33±9.71)s、(341.00±12.77)s和650 m A组(237.67±16.29)s、(320.00±16.52)s;其平均单次脱毛成功率分别为(66.67±5.77)%和(53.33±5.77)%,低于600 m A(83.33±5.77)%、(73.33±11.55)%和650 m A组(86.67±11.55)%,(80.00±10.00)%,P<0.05;眉毛在不同电流下的平均脱毛时长和单次脱毛成功率差异无统计学意义。电解后毛发的毛发镜检查示:毛发结构基本完整、可见残留的外毛根鞘组织。头皮肿物患者皮损边缘的毛发予电解去除后行活检,其组织病理检查示毛囊周围纵向裂隙,毛囊细胞变性坏死、周围结缔组织结构大致正常。电解成功去除10例白癜风患者的15处白发,术中白发去除率近100%,3个月随访时平均白发新生率为(9.1±6.3)%。采用FUE治疗4例白癜风患者4处白发,术中平均白发去除率(67.2±23.31)%,术中出血多,术后3月平均黑发成活率61.23±18.27%,术后1年随访时平均黑发成活率(74.4±19.9)%,其中1例患者出现轻度萎缩性瘢痕、毛发密度减少和局部感觉异常。采用电解联合自体表皮移植治疗1例枕部白斑伴白发患者,术中白发完全去除,3个月随访时白斑复色程度≥75%,白发新生率13.3%(32/240)。电解联合FUE治疗4例白癜风患者4处白发,平均术中白发去除率为(98.33±3.35)%,3个月随访时平均黑发成活率(90.7±4.73)%。联合治疗患者术后未见瘢痕、毛发密度减少等不良反应,患者满意度高。结论倒睫电解器可以通过精准破坏毛囊组织去除白癜风白发,去除率高且不良反应轻微,而电解联合外科移植则可提高稳定期白癜风白发的治疗效果,值得临床应用推广。展开更多
Background Topical tacrolimus has been used for vitiligo as a common treatment option for more than ten years while the underlying mechanism is still uncertain.The aim of this study was to investigate the direct effec...Background Topical tacrolimus has been used for vitiligo as a common treatment option for more than ten years while the underlying mechanism is still uncertain.The aim of this study was to investigate the direct effects of tacrolimus on the melanogenesis and migration on human A375 melanoma cells.The expression of c-KIT mRNA and protein of human A375 cells were also investigated.Methods The cultured A375 human melanoma cells were randomly assigned to control and tacrolimus treatment groups （10,102,103and 104 nmol/L）.The cell proliferation was measured with Cell Counting Kit-8 assays.Melanin content was measured with NaOH method.Transwell migration assay was used to measure cell migration.The expression of c-KIT mRNA and protein were measured with real-time fluorescence quantitative polymerase chain reaction and immunohistochemistry respectively.Results The cell proliferation of the 103 and 104 nmol/L tacrolimus groups were significantly lower （0.666±0.062 and 0.496±0.038） as compared with the control （0.841±0.110,P 〈0.05）.The mean melanin content in all groups treated with different concentration of tacrolimus （10,102,103,104 nmol/L） increased compared with the control group （P 〈0.05）.Dosedependent increase in cell migration were seen in all tacrolimus-treated groups （P 〈0.01）.The expression of c-KIT mRNA level in A375 cells exposed to tacrolimus （103and 104 nmol/L） had significantly increased by 3.03-fold and 3.19-fold respectively compared with the control （P 〈0.05）.Conclusions Although tacrolimus had no effects on cell proliferation on A375 human melanoma cells,it could increase the melanin content and cell migration.The expression of c-KIT mRNA and protein increased dose-dependently in tacrolimus-treated groups as compared with the control.Our study demonstrated that tacrolimus could enhance the melanogenesis and cell migration on A375 cells.展开更多
文摘目的探索倒睫电解器用于去除白发的可行性,探讨电解联合外科移植治疗不同程度白癜风白发的临床应用价值。方法对健康志愿者头皮及眉毛受试区在不同电流下进行脱毛,观察平均脱毛时长、单次脱毛成功率,明确不同部位脱毛的最适电流值,并观察电解后毛发镜及组织病理学改变;分别采用单独电解、单独毛囊单位提取术(follicular unit extraction,FUE),和电解联合外科移植法(自体表皮移植术/FUE)治疗稳定期白癜风白发,随访3个月,评价疗效、术后恢复时间及不良反应。结果健康志愿者的头部黑发和白发在550 m A电流下的平均脱毛时长分别为(278.00±14.8)s和(373.00±8.19)s,均高于600 m A(248.33±9.71)s、(341.00±12.77)s和650 m A组(237.67±16.29)s、(320.00±16.52)s;其平均单次脱毛成功率分别为(66.67±5.77)%和(53.33±5.77)%,低于600 m A(83.33±5.77)%、(73.33±11.55)%和650 m A组(86.67±11.55)%,(80.00±10.00)%,P<0.05;眉毛在不同电流下的平均脱毛时长和单次脱毛成功率差异无统计学意义。电解后毛发的毛发镜检查示:毛发结构基本完整、可见残留的外毛根鞘组织。头皮肿物患者皮损边缘的毛发予电解去除后行活检,其组织病理检查示毛囊周围纵向裂隙,毛囊细胞变性坏死、周围结缔组织结构大致正常。电解成功去除10例白癜风患者的15处白发,术中白发去除率近100%,3个月随访时平均白发新生率为(9.1±6.3)%。采用FUE治疗4例白癜风患者4处白发,术中平均白发去除率(67.2±23.31)%,术中出血多,术后3月平均黑发成活率61.23±18.27%,术后1年随访时平均黑发成活率(74.4±19.9)%,其中1例患者出现轻度萎缩性瘢痕、毛发密度减少和局部感觉异常。采用电解联合自体表皮移植治疗1例枕部白斑伴白发患者,术中白发完全去除,3个月随访时白斑复色程度≥75%,白发新生率13.3%(32/240)。电解联合FUE治疗4例白癜风患者4处白发,平均术中白发去除率为(98.33±3.35)%,3个月随访时平均黑发成活率(90.7±4.73)%。联合治疗患者术后未见瘢痕、毛发密度减少等不良反应,患者满意度高。结论倒睫电解器可以通过精准破坏毛囊组织去除白癜风白发,去除率高且不良反应轻微,而电解联合外科移植则可提高稳定期白癜风白发的治疗效果,值得临床应用推广。
文摘Background Topical tacrolimus has been used for vitiligo as a common treatment option for more than ten years while the underlying mechanism is still uncertain.The aim of this study was to investigate the direct effects of tacrolimus on the melanogenesis and migration on human A375 melanoma cells.The expression of c-KIT mRNA and protein of human A375 cells were also investigated.Methods The cultured A375 human melanoma cells were randomly assigned to control and tacrolimus treatment groups （10,102,103and 104 nmol/L）.The cell proliferation was measured with Cell Counting Kit-8 assays.Melanin content was measured with NaOH method.Transwell migration assay was used to measure cell migration.The expression of c-KIT mRNA and protein were measured with real-time fluorescence quantitative polymerase chain reaction and immunohistochemistry respectively.Results The cell proliferation of the 103 and 104 nmol/L tacrolimus groups were significantly lower （0.666±0.062 and 0.496±0.038） as compared with the control （0.841±0.110,P 〈0.05）.The mean melanin content in all groups treated with different concentration of tacrolimus （10,102,103,104 nmol/L） increased compared with the control group （P 〈0.05）.Dosedependent increase in cell migration were seen in all tacrolimus-treated groups （P 〈0.01）.The expression of c-KIT mRNA level in A375 cells exposed to tacrolimus （103and 104 nmol/L） had significantly increased by 3.03-fold and 3.19-fold respectively compared with the control （P 〈0.05）.Conclusions Although tacrolimus had no effects on cell proliferation on A375 human melanoma cells,it could increase the melanin content and cell migration.The expression of c-KIT mRNA and protein increased dose-dependently in tacrolimus-treated groups as compared with the control.Our study demonstrated that tacrolimus could enhance the melanogenesis and cell migration on A375 cells.
