目的:探讨云木香乙醇提取物(ethanol extract of costusroot,EEC)对胃溃疡模型大鼠胃黏膜的影响及相关机制。方法:将SD大鼠随机分组:正常组、模型组、硫糖铝组、EEC低、高剂量组,除正常组外其余各组大鼠灌胃无水乙醇复制胃溃疡模型。观...目的:探讨云木香乙醇提取物(ethanol extract of costusroot,EEC)对胃溃疡模型大鼠胃黏膜的影响及相关机制。方法:将SD大鼠随机分组:正常组、模型组、硫糖铝组、EEC低、高剂量组,除正常组外其余各组大鼠灌胃无水乙醇复制胃溃疡模型。观察各组大鼠胃溃疡面积变化并计算溃疡抑制率;HE染色法观测胃组织病理形态学改变;采用免疫组化法(immunohistochemisty,IHC)和Western blot法检测胃溃疡大鼠胃组织中Bax、Bcl-2蛋白的表达水平。结果:EEC能有效抑制模型大鼠溃疡面积的形成,减轻乙醇引起的胃组织病理学损伤;上调胃溃疡大鼠胃组织中Bcl-2蛋白表达(P<0.01),有效抑制乙醇引起的Bax蛋白过量表达(P<0.01)。结论:EEC具有良好的抗胃溃疡作用,其作用机制可能与调节Bax、Bcl-2蛋白表达水平抑制胃黏膜细胞凋亡有关。展开更多
OBJECTIVE:To investigate the effect of ethanol extracts of Muxiang(Radix Aucklandiae)(RA)on gastric ulcers in rats and explore the potential mechanisms.METHODS:A model was established by ethanol(0.75 m L/kg).According...OBJECTIVE:To investigate the effect of ethanol extracts of Muxiang(Radix Aucklandiae)(RA)on gastric ulcers in rats and explore the potential mechanisms.METHODS:A model was established by ethanol(0.75 m L/kg).According to body weight,rats were pretreated with RA extracts(2.5 or 5 g/kg).The rats were administered 95%ethanol orally after 1 h.The effects of ethanol were evaluated by measuring the gastric secretion volume,p H,pepsin activity,and ulcer area.Histological analysis and immunohistochemistry were also conducted.Furthermore,the effect of the ethanol extract of RA on transiting activity of the gastrointestinal tract was observed in mice.RESULTS:Intragastric administration of RA extracts protected the gastric mucosa from ethanol-induced gastric ulcers,while reducing submucosal edema and preventing hemorrhagic damage.Moreover,the extracts increased the production of gastric mucus,upregulated Bcl-2,and downregulated Bax expression.Importantly,pretreated rats exhibited no significant change in the gastric secretion volume,gastric juice acidity,or pepsin.Furthermore,pretreatment prominently(P<0.05)enhanced propulsive movement of the gastrointestinal tract in normal mice and mice with gastrointestinal motility disorders.CONCLUSION:Ethanol extracts of RA ameliorated gastric lesions in the gastric ulcer rat model.The mechanisms of action were related to improvement of gastrointestinal dynamics,maintenance of mucus integrity,and inhibition of apoptosis by downregulating proapoptotic Bax protein and upregulating anti-apoptotic Bcl-2 protein.展开更多
文摘目的:探讨云木香乙醇提取物(ethanol extract of costusroot,EEC)对胃溃疡模型大鼠胃黏膜的影响及相关机制。方法:将SD大鼠随机分组:正常组、模型组、硫糖铝组、EEC低、高剂量组,除正常组外其余各组大鼠灌胃无水乙醇复制胃溃疡模型。观察各组大鼠胃溃疡面积变化并计算溃疡抑制率;HE染色法观测胃组织病理形态学改变;采用免疫组化法(immunohistochemisty,IHC)和Western blot法检测胃溃疡大鼠胃组织中Bax、Bcl-2蛋白的表达水平。结果:EEC能有效抑制模型大鼠溃疡面积的形成,减轻乙醇引起的胃组织病理学损伤;上调胃溃疡大鼠胃组织中Bcl-2蛋白表达(P<0.01),有效抑制乙醇引起的Bax蛋白过量表达(P<0.01)。结论:EEC具有良好的抗胃溃疡作用,其作用机制可能与调节Bax、Bcl-2蛋白表达水平抑制胃黏膜细胞凋亡有关。
基金Supported by the Science and Technique Plan of the Education Department of Yunnan Province:the Effect of the Ethanol Extract of Muxiang(Radix Aucklandiae)on Gastric Ulcer in Rats(No.2018JS285)Yunnan Innovation Team of Application Research on Tradtional Chinese Medicine Theory of Disease Prevention at Yunnan University of TCM(No.2017HC011)Yunnan Provincial University Key Laboratory of Aromatic Chinese Herb Research.
文摘OBJECTIVE:To investigate the effect of ethanol extracts of Muxiang(Radix Aucklandiae)(RA)on gastric ulcers in rats and explore the potential mechanisms.METHODS:A model was established by ethanol(0.75 m L/kg).According to body weight,rats were pretreated with RA extracts(2.5 or 5 g/kg).The rats were administered 95%ethanol orally after 1 h.The effects of ethanol were evaluated by measuring the gastric secretion volume,p H,pepsin activity,and ulcer area.Histological analysis and immunohistochemistry were also conducted.Furthermore,the effect of the ethanol extract of RA on transiting activity of the gastrointestinal tract was observed in mice.RESULTS:Intragastric administration of RA extracts protected the gastric mucosa from ethanol-induced gastric ulcers,while reducing submucosal edema and preventing hemorrhagic damage.Moreover,the extracts increased the production of gastric mucus,upregulated Bcl-2,and downregulated Bax expression.Importantly,pretreated rats exhibited no significant change in the gastric secretion volume,gastric juice acidity,or pepsin.Furthermore,pretreatment prominently(P<0.05)enhanced propulsive movement of the gastrointestinal tract in normal mice and mice with gastrointestinal motility disorders.CONCLUSION:Ethanol extracts of RA ameliorated gastric lesions in the gastric ulcer rat model.The mechanisms of action were related to improvement of gastrointestinal dynamics,maintenance of mucus integrity,and inhibition of apoptosis by downregulating proapoptotic Bax protein and upregulating anti-apoptotic Bcl-2 protein.