Translation and validation of the Nepean Dyspepsia Index for functional dyspepsia in China

AIM： To assess the reliability and validity of the translated version of Nepean Dyspepsia Index （NDI） in Chinese patients with documented functional dyspepsia （FO）. METHODS： The translation process included forward translation, back translation, pretest and cross-cultural adaptation. Reliability and validity of the translated version were examined by asking 300 subjects to complete the Chinese version of the NDI. The mean age of subjects was 39.24 years and 68.7% of the subjects were women. Internal consistency analysis with Cronbach＇s α was performed to test the reliability. Correlation analysis was used to assess the content validity. Factor analysis and structural equation models were used to assess the construct validity. RESULTS： The Cronbach＇s α coefficients ranged 0.833-0.960, well above the acceptable level of 0.70. Correlation analysis showed that each item had a strong correlation with the corresponding domain, but a weak correlation with other domains. Confirmatory factor analysis indicated that the comparative fit index was 0.94, higher than the acceptable level of 0.90.CONCLUSION： The Chinese version of the NDI is a reliable and valid scale for measuring health-related quality of life and disease severity in Chinese patients with FD.

Based on necroptosis identifying the immunological features and prognostic signatures of lung adenocarcinoma

Background:Lung adenocarcinoma is one of the most common pathological types of lung malignant tumor with high morbidity and mortality.Long non-coding RNAs are gradually recognized to play crucial roles in tumor occurrence and development.Necroptosis is a newly established way for cell programmed death,undertaking essential roles in anti-tumor.Therefore,identifying necroptosis-related l ong non-coding RNAs and based on them to evaluate the signatures of l ung adenocarcinoma is essential for patients’survival prediction and therapy.Methods:We collected data from the public database and performed the least absolute shrinkage to construct a 13-lncRNAs prognostic model.Based on the Consensus Clustering,ESTIMATE,CIRERSORT,and weighted gene co-expression network analysis to identify the immune signatures.Results:This study identified a 13-lncRNAs prognostic model.The model’s prediction accuracy was evaluated by receiver operating characteristic and independent-prognosis analysis;besides,a Gene Expression Omnibus dataset was applied for external validation.Furthermore,we analyzed the immune features of subgroups in multiple dimensions.A consensus clustering analysis based on the 41 genes was implemented to separate lung adenocarcinoma patients into two subgroups.We compared the features of subgroups in multiple dimensions,including survival,immune microenvironment,immune cells infiltration and gene co-expression network analysis.Conclusion:W e established a prognosis necroptosis-related risk model to predict lung adenocarcinoma patients’prognosis and systematically understood the correlation between immune and necroptosis.This study can applicate in clinical to predict the prognosis of lung adenocarcinoma patients and provide new insight into lung adenocarcinoma immune therapy.

Case of multiple verruca plantaris

A female student,23 years old,suffering from multiple warts scattered on the right sole for 2 years and pain on the bottom of the first metatarsal bone when walking for 1 month.The case was diagnosed as kujinjian(枯筋箭)in traditional Chinese medicine and verruca plantaris in western medicine.At the beginning of treatment,fire needling therapy was applied to local lesions,once every two days.Afterwards,moxibustion with moxa cone was used at the local lesions,once a day,three cones on each lesion till warts were dropped.Simultaneously,in combination,mild moxibustion was given at bilateral Zusanli(足三里ST36)for 15 min,once a day.After three times of moxibustion therapy,a part of warts were crusted and 3 weeks later,all the warts were dropped.No recurrence was found in the follow-up visit.The therapeutic methods of this case are simple in manipulation and satisfactory in therapeutic effect.