Objective: To study the correlation of PDCD4 and telomerase expression with the expression of apoptosis molecules and interstitial molecules in myocardial tissue of sudden coronary death. Methods: The sudden death cas...Objective: To study the correlation of PDCD4 and telomerase expression with the expression of apoptosis molecules and interstitial molecules in myocardial tissue of sudden coronary death. Methods: The sudden death cases that underwent autopsy in the Fifth Affiliated Hospital of Southern Medical University between March 2014 and March 2017 were collected and divided into group A with sudden cardiac death of coronary heart disease, group B with sudden non-cardiac death of coronary heart disease and group C without coronary lesions according to the autopsy results and medicolegal expertise report. The myocardial tissue was collected to determine the mRNA expression of PDCD4 and telomerase as well as the protein expression of PDCD4, telomerase, apoptosis molecules and interstitial molecules. Results:The mRNA expression and protein expression of PDCD4 and telomerase in myocardial tissue of group A were significantly higher than those of group B and group C;Bax, p53 and SOCS1 protein expression in myocardial tissue of group A were higher than those in group B and group C and positively correlated with PDCD4 and telomerase protein expression whereas Bcl-2, N-cadherin, Cx40, Cx43, Cx45 and FN protein expression were lower than those of group B and group C and negatively correlated with PDCD4 and telomerase protein expression;the mRNA expression and protein expression of PDCD4 and telomerase as well as the protein expression of Bax, Bcl-2, p53, SOCS1, N-cadherin, Cx40, Cx43, Cx45 and FN in myocardial tissue were not significantly different between group B and Group C. Conclusion:The high expression of PDCD4 and telomerase is related to the sudden cardiac death of coronary heart disease, and it regulates the expression of apoptosis and interstitial molecules in myocardial tissue.展开更多
文摘Objective: To study the correlation of PDCD4 and telomerase expression with the expression of apoptosis molecules and interstitial molecules in myocardial tissue of sudden coronary death. Methods: The sudden death cases that underwent autopsy in the Fifth Affiliated Hospital of Southern Medical University between March 2014 and March 2017 were collected and divided into group A with sudden cardiac death of coronary heart disease, group B with sudden non-cardiac death of coronary heart disease and group C without coronary lesions according to the autopsy results and medicolegal expertise report. The myocardial tissue was collected to determine the mRNA expression of PDCD4 and telomerase as well as the protein expression of PDCD4, telomerase, apoptosis molecules and interstitial molecules. Results:The mRNA expression and protein expression of PDCD4 and telomerase in myocardial tissue of group A were significantly higher than those of group B and group C;Bax, p53 and SOCS1 protein expression in myocardial tissue of group A were higher than those in group B and group C and positively correlated with PDCD4 and telomerase protein expression whereas Bcl-2, N-cadherin, Cx40, Cx43, Cx45 and FN protein expression were lower than those of group B and group C and negatively correlated with PDCD4 and telomerase protein expression;the mRNA expression and protein expression of PDCD4 and telomerase as well as the protein expression of Bax, Bcl-2, p53, SOCS1, N-cadherin, Cx40, Cx43, Cx45 and FN in myocardial tissue were not significantly different between group B and Group C. Conclusion:The high expression of PDCD4 and telomerase is related to the sudden cardiac death of coronary heart disease, and it regulates the expression of apoptosis and interstitial molecules in myocardial tissue.
