A three-dimensional matrix system containing melatonin and neural stem cells repairs damage from traumatic brain injury in rats

Brain lesions can cause neural stem cells to activate,proliferate,diffe rentiate,and migrate to the injured area.However,after traumatic brain injury,brain tissue defects and microenvironment changes greatly affect the survival and growth of neural stem cells;the resulting reduction in the number of neural stem cells impedes effective repair of the injured area.Melatonin can promote the survival,proliferation,and differentiation of neural stem cells under adverse conditions such as oxidative stress or hypoxia that can occur after traumatic brain injury.Therefore,we investigated the therapeutic effects of melatonin combined with neural stem cells on traumatic brain injury in rats.First,in vitro studies confirmed that melatonin promoted the survival of neural stem cells deprived of oxygen and glucose.Then,we established a three-dimensional Matrigel-based transplantation system containing melatonin and neural stem cells and then used it to treat traumatic brain injury in rats.We found that treatment with the Matrigel system containing melatonin and neural stem cells decreased brain lesion volume,increased the number of surviving neuro ns,and improved recove ry of neurological function compared with treatment with Matrigel alone,neural stem cells alone,Matrigel and neural stem cells combined,and Matrigel and melatonin combined.Our findings suggest that the three-dimensional Matrigelbased transplantation system containing melatonin and neural stem cells is a potential treatment for traumatic brain injury.

Optimal Design of Non-axisymmetric Endwall with Variable-fidelity CFD Model

Non-axisymmetric endwall is numerically investigated to suppress the secondary endwall flow in a HP turbine suction surface.The endwall contour is represented by a non-uniform rational B-spline surface.Two solution spaces are designed by the rule of hypercube sampling through variation of nodal radial extension.Final most effective endwall contour is obtained through screening all available samples with surrogate-based model.Results show that non axisymmetric endwall reduces the turbulence kinetic energy of the passage vortex by 5.5%and the total pressure loss 1.6%.Local heat transfer coefficient is lowered on the afore passage endwall surface with a certain increment on the aft passage endwall.Overall averaged heat transfer coefficient is reduced.

Dynamic Reliability Analysis of Braced Frame Structures Considering Inter Story Correlation

Adding buckling restrained braces(BRB)of reinforced concrete frame structure can effectively improve the safety performance of the structure.The dynamic reliability analysis based on Poisson continuous process assumption and the first exceeding failure probability can be used to obtain the failure probability of the buckling restrained brace frame system under earthquake load,and the relationship between the failure probabilities of each floor of the structure is analyzed to obtain the frame system reliability interval of frame structure.The results show that the reliability of BRB frame structure is higher than that of pure frame structure,and the discrete failure probability is lower.