In order to find landscape plants purifying Pb pollution of soil,a pot experiment was conducted to study Pb resistance and enrichment characteristics of six species of Compositae plants( Aster ageratoides,Rudbeckia hi...In order to find landscape plants purifying Pb pollution of soil,a pot experiment was conducted to study Pb resistance and enrichment characteristics of six species of Compositae plants( Aster ageratoides,Rudbeckia hirta,Cosmos sulphureus,Gynura bicolor,Calendula officinalis,Bidens pilosa) in different soil Pb concentrations. The results showed that the dry weight of aboveground part in the six plants decreased with the increase of soil Pb concentration. The tolerance index( TI) of C. sulphureus,G. bicolor and C. officinalis was greater than 0. 5 in all concentrations,and the root tolerance index( RTI) was greater than 0. 9,which were better resistant to Pb stress. The maximum Pb content in aboveground part of R. hirta was 1 783 mg/kg,and the enrichment and transport coefficients were all greater than 1. When the soil Pb concentration was 500 mg/kg,the total Pb accumulation in the aboveground part of R. hirta was 2. 507 mg/plant. Finally based on the integrated observation of the enrichment coefficient,transport factor and repair potential index,it believed that R. hirta can be used to restore Pb contaminated soil at low concentration.展开更多
目的探究远中缺失同源盒基因5（distal-less homeobox genes 5，Dlx-5）和肌节同源盒基因1（Msh homeobox 1，Msx-1）在双膦酸盐药物性颌骨坏死（bisphosphonate-associated osteonecrosis of the jaw，BRONJ）致病机制中的作用。方法...目的探究远中缺失同源盒基因5（distal-less homeobox genes 5，Dlx-5）和肌节同源盒基因1（Msh homeobox 1，Msx-1）在双膦酸盐药物性颌骨坏死（bisphosphonate-associated osteonecrosis of the jaw，BRONJ）致病机制中的作用。方法将24只SD大鼠采用随机数字表法随机分为两组，每组12只。实验组腹腔注射唑来膦酸0.2 mg/kg，对照组腹腔注射等量生理盐水，每周3次，12周后拔除左下第一磨牙。拔牙后8周处死所有动物。通过影像学、大体及组织病理学观察，诊断BRONJ的发生。通过实时荧光PCR检测Dlx-5和Msx-1在骨坏死样本中的表达水平。结果BRONJ动物模型中，实验组Dlx-5基因表达（5.91±0.28）显著高于对照组（1.00±0.15）（t=15.778，P=0.001），实验组Msx-1基因表达（0.09±0.03）显著低于对照组（1.02±0.06）（t=-10.638，P=0.001）。结论腹腔注射唑来膦酸联合拔牙的方法可成功诱导大鼠发生BRONJ，Dlx-5和Msx-1基因在BRONJ的致病过程中发挥作用。展开更多
[目的]探讨miR-655对结直肠癌细胞奥沙利铂化疗敏感性的影响。[方法]通过q RT-PCR验证奥沙利铂耐药和敏感结直肠癌组织蜡块中miR-655的相对表达量;CCK-8法检测细胞活性;Transwell检测细胞侵袭能力;流式细胞术检测细胞周期。[结果]相比...[目的]探讨miR-655对结直肠癌细胞奥沙利铂化疗敏感性的影响。[方法]通过q RT-PCR验证奥沙利铂耐药和敏感结直肠癌组织蜡块中miR-655的相对表达量;CCK-8法检测细胞活性;Transwell检测细胞侵袭能力;流式细胞术检测细胞周期。[结果]相比于癌旁的正常组织,对奥沙利铂耐药的结直肠癌组织中miR-655表达量相对较低(1.24±0.28 vs0.25±0.12,P<0.01),而对奥沙利铂敏感的结直肠癌组织中miR-655表达量相对较高(1.24±0.28 vs 2.68±0.76,P<0.01)。QRT-PCR鉴定miR-655转染效率结果显示,在miR-655 mimics组中miR-655表达水平相对于miR-NC组显著升高(1.70±0.10 vs 0.83±0.07,P=0.004)。CCK-8检测细胞活性结果显示,与L-OHP/miR-NC组相比,L-OHP/miR-655 mimics组细胞活性降低(48h:0.79±0.05 vs 0.56±0.03,P=0.0015;72h:0.65±0.05 vs 0.25±0.05,P=0.0081)。Transwell法检测细胞的侵袭能力结果显示,与L-OHP/miR-NC组相比,L-OHP/miR-655 mimic组细胞侵袭数量减少(845±85 vs 613±36,P=0.0292)。流式细胞术检测细胞周期结果显示,与L-OHP/miRNC组G0/G1期相比,L-OHP/miR-655 mimics组细胞比例增加(5.16±0.22 vs 45.53±0.75,P=0.022),与L-OHP/miR-NC组S期相比,L-OHP/miR-655 mimics组细胞比例降低(44.83±1.17 vs20.75±0.36,P=0.015),与L-OHP/miR-NC组G2/M期相比,L-OHP/miR-655 mimics组细胞比例减少(22.86±1.21 vs 3.85±0.35,P=0.0023)。[结论]MiR-655能够提高结直肠癌细胞对奥沙利铂的化疗敏感性,miR-655可能成为联同奥沙利铂治疗结直肠癌的新靶点。展开更多
基金Supported by Science and Technology Support Project in Guizhou Province(Qiankehezhicheng[2017]2580)Key Projects of Guizhou Science and Technology Fund(Qiankehe JZ[2014]2012)
文摘In order to find landscape plants purifying Pb pollution of soil,a pot experiment was conducted to study Pb resistance and enrichment characteristics of six species of Compositae plants( Aster ageratoides,Rudbeckia hirta,Cosmos sulphureus,Gynura bicolor,Calendula officinalis,Bidens pilosa) in different soil Pb concentrations. The results showed that the dry weight of aboveground part in the six plants decreased with the increase of soil Pb concentration. The tolerance index( TI) of C. sulphureus,G. bicolor and C. officinalis was greater than 0. 5 in all concentrations,and the root tolerance index( RTI) was greater than 0. 9,which were better resistant to Pb stress. The maximum Pb content in aboveground part of R. hirta was 1 783 mg/kg,and the enrichment and transport coefficients were all greater than 1. When the soil Pb concentration was 500 mg/kg,the total Pb accumulation in the aboveground part of R. hirta was 2. 507 mg/plant. Finally based on the integrated observation of the enrichment coefficient,transport factor and repair potential index,it believed that R. hirta can be used to restore Pb contaminated soil at low concentration.
文摘[目的]探讨miR-655对结直肠癌细胞奥沙利铂化疗敏感性的影响。[方法]通过q RT-PCR验证奥沙利铂耐药和敏感结直肠癌组织蜡块中miR-655的相对表达量;CCK-8法检测细胞活性;Transwell检测细胞侵袭能力;流式细胞术检测细胞周期。[结果]相比于癌旁的正常组织,对奥沙利铂耐药的结直肠癌组织中miR-655表达量相对较低(1.24±0.28 vs0.25±0.12,P<0.01),而对奥沙利铂敏感的结直肠癌组织中miR-655表达量相对较高(1.24±0.28 vs 2.68±0.76,P<0.01)。QRT-PCR鉴定miR-655转染效率结果显示,在miR-655 mimics组中miR-655表达水平相对于miR-NC组显著升高(1.70±0.10 vs 0.83±0.07,P=0.004)。CCK-8检测细胞活性结果显示,与L-OHP/miR-NC组相比,L-OHP/miR-655 mimics组细胞活性降低(48h:0.79±0.05 vs 0.56±0.03,P=0.0015;72h:0.65±0.05 vs 0.25±0.05,P=0.0081)。Transwell法检测细胞的侵袭能力结果显示,与L-OHP/miR-NC组相比,L-OHP/miR-655 mimic组细胞侵袭数量减少(845±85 vs 613±36,P=0.0292)。流式细胞术检测细胞周期结果显示,与L-OHP/miRNC组G0/G1期相比,L-OHP/miR-655 mimics组细胞比例增加(5.16±0.22 vs 45.53±0.75,P=0.022),与L-OHP/miR-NC组S期相比,L-OHP/miR-655 mimics组细胞比例降低(44.83±1.17 vs20.75±0.36,P=0.015),与L-OHP/miR-NC组G2/M期相比,L-OHP/miR-655 mimics组细胞比例减少(22.86±1.21 vs 3.85±0.35,P=0.0023)。[结论]MiR-655能够提高结直肠癌细胞对奥沙利铂的化疗敏感性,miR-655可能成为联同奥沙利铂治疗结直肠癌的新靶点。
