Periodontitis is a widespread oral disease characterized by continuous inflammation of the periodontal tissue and an irreversible alveolar bone loss, which eventually leads to tooth loss. Four-octyl itaconate(4-OI) is...Periodontitis is a widespread oral disease characterized by continuous inflammation of the periodontal tissue and an irreversible alveolar bone loss, which eventually leads to tooth loss. Four-octyl itaconate(4-OI) is a cell-permeable itaconate derivative and has been recognized as a promising therapeutic target for the treatment of inflammatory diseases. Here, we explored, for the first time,the protective effect of 4-OI on inhibiting periodontal destruction, ameliorating local inflammation, and the underlying mechanism in periodontitis. Here we showed that 4-OI treatment ameliorates inflammation induced by lipopolysaccharide in the periodontal microenvironment. 4-OI can also significantly alleviate inflammation and alveolar bone loss via Nrf2 activation as observed on samples from experimental periodontitis in the C57BL/6 mice. This was further confirmed as silencing Nrf2 blocked the antioxidant effect of 4-OI by downregulating the expression of downstream antioxidant enzymes. Additionally, molecular docking simulation indicated the possible mechanism under Nrf2 activation. Also, in Nrf2-/-mice, 4-OI treatment did not protect against alveolar bone dysfunction due to induced periodontitis, which underlined the importance of the Nrf2 in 4-OI mediated periodontitis treatment.Our results indicated that 4-OI attenuates inflammation and oxidative stress via disassociation of KEAP1-Nrf2 and activation of Nrf2 signaling cascade. Taken together, local administration of 4-OI offers clinical potential to inhibit periodontal destruction,ameliorate local inflammation for more predictable periodontitis.展开更多
Species in the genus Xenogryllus may be well-known to Chinese citizens since their calling songs often attract people to feed and enjoy them. Two species, Xenogryllus transversus (Walker, 1869) and Xenogryllus marmora...Species in the genus Xenogryllus may be well-known to Chinese citizens since their calling songs often attract people to feed and enjoy them. Two species, Xenogryllus transversus (Walker, 1869) and Xenogryllus marmoratus (Haan, 1842), were known to reside in China from previous studies. However, the distribution of X. transversus in China derives from misidentification. Specimens from Yunnan Province, misidentified as X. transversus in earlier studies, are recognized as Xenogryllus maichauensis Gorochov, 1992 in this study. Another large Xenogryllus species found from Guangdong Province is identified as X. ululiu Gorochov, 1990. Both species are recorded in China for the first time. In this article, all known species of genus Xenogryllus from China are described and illustrated and a key to Chinese species of Xenogryllus is presented.展开更多
基金supported by the National Natural Science Foundation of China (31971282 and 32071362)2019 Chongqing Graduate Tutor Team Construction Project (dstd201903)Scientific and Technological Research Program of Chongqing Municipal Education Commission (KJQN201900415)。
文摘Periodontitis is a widespread oral disease characterized by continuous inflammation of the periodontal tissue and an irreversible alveolar bone loss, which eventually leads to tooth loss. Four-octyl itaconate(4-OI) is a cell-permeable itaconate derivative and has been recognized as a promising therapeutic target for the treatment of inflammatory diseases. Here, we explored, for the first time,the protective effect of 4-OI on inhibiting periodontal destruction, ameliorating local inflammation, and the underlying mechanism in periodontitis. Here we showed that 4-OI treatment ameliorates inflammation induced by lipopolysaccharide in the periodontal microenvironment. 4-OI can also significantly alleviate inflammation and alveolar bone loss via Nrf2 activation as observed on samples from experimental periodontitis in the C57BL/6 mice. This was further confirmed as silencing Nrf2 blocked the antioxidant effect of 4-OI by downregulating the expression of downstream antioxidant enzymes. Additionally, molecular docking simulation indicated the possible mechanism under Nrf2 activation. Also, in Nrf2-/-mice, 4-OI treatment did not protect against alveolar bone dysfunction due to induced periodontitis, which underlined the importance of the Nrf2 in 4-OI mediated periodontitis treatment.Our results indicated that 4-OI attenuates inflammation and oxidative stress via disassociation of KEAP1-Nrf2 and activation of Nrf2 signaling cascade. Taken together, local administration of 4-OI offers clinical potential to inhibit periodontal destruction,ameliorate local inflammation for more predictable periodontitis.
基金supported by the National Natural Science Foundation of China (31750002)the Fundamental Research Funds for the Central Universities (GK201702010)
文摘Species in the genus Xenogryllus may be well-known to Chinese citizens since their calling songs often attract people to feed and enjoy them. Two species, Xenogryllus transversus (Walker, 1869) and Xenogryllus marmoratus (Haan, 1842), were known to reside in China from previous studies. However, the distribution of X. transversus in China derives from misidentification. Specimens from Yunnan Province, misidentified as X. transversus in earlier studies, are recognized as Xenogryllus maichauensis Gorochov, 1992 in this study. Another large Xenogryllus species found from Guangdong Province is identified as X. ululiu Gorochov, 1990. Both species are recorded in China for the first time. In this article, all known species of genus Xenogryllus from China are described and illustrated and a key to Chinese species of Xenogryllus is presented.
