m7G Methylation-Related Gene NUDT10 as novel prognostic biomarker and its ceRNA regulatory network in colorectal cancer

Background:Colorectal cancer(CRC)is a life-threatening malignant disease of the digestive system,which is still a leading cause of cancer death worldwide.RNA methylation,a fundamental process in epigenetic regulation,also plays an important role in tumor development.Nevertheless,the functional mechanisms of 7-methylguanosine(m7G)methylation-related gene in CRC are unclear.Methods:The differential expression of m7G methylation-related gene between CRC tissues and normal tissues in UCSC XENA,TCGA and GTEx was verified using R.Cox regression,Nomogram were used to construct corresponding prognostic models of CRC.Kaplan-Meier analysis,receiver operating characteristic curve analysis,clinical correlation analysis and enrichment analysis were used to further investigate the correlation and functional mechanisms of m7G methylation-related gene with CRC.Immuno-infiltration was used to verify the relationship between nudix hydroxylase 10(NUDT10)and immune cells,while methylation analysis was used to study the promoter methylation of NUDT10.Immunohistochemistry was used to analyze the protein expression between CRC tissues and normal tissues.The ceRNA regulatory network was used to identify the mRNA-miRNA-lncRNA regulatory axis.Results:The m7G methylation-related gene NUDT10 was lowly expressed in CRC.Cox regression,Nomogram and calibration curve finally identified NUDT10 as the novel prognostic biomarker of CRC and constructed the corresponding prognostic models.The results of immunohistochemistry showed that the protein expression levels of NUDT10 differed between CRC tissues and normal tissues.In addition,NUDT10 had predictive power for CRC and the expression of NUDT10 in CRC was closely associated with immune infiltration.Gene Ontology,Kyoto Encyclopedia of Genes and Genomes and Gene Set Enrichment Analysis revealed that biological functions such as the regulation of angiogenesis,muscle contraction,transporter complex and gated channel activity,pathways such as heme metabolism,hypoxia,cholinergic synapse,apical junction and epithelial mesenchymal transition all play a role in the development of CRC.The study of methylation revealed that NUDT10 showed hyper-methylation in both CRC and normal tissues,in which the promoter methylation of DNA in CRC was lower than that in normal colon and rectum.The ceRNA regulatory network revealed that 3 lncRNAs such as XIST,interfered the expression of NUDT10 through 6 miRNAs such as hsa-miR-23a-3p,and then affected the development of CRC.Conclusion:The m7G methylation-related gene NUDT10 can be used as novel biomarker in CRC.A total of 3 lncRNAs,including XIST,are involved in the expression of NUDT10 in CRC through 6 miRNAs such as hsa-miR-23a-3p.

Evaluation of the Effect of Shenmai Injection on Chemotherapy Efficacy and Side Effects of Acute Leukemia

Objective.To study the effect of Shenmai injection on the efficacy and side effects of chemotherapy in acute leukemia.Methods.Sixty-two patients with acute leukemia admitted to the hospital between February 2018 and June 2019 were enrolled in this study.All patients were divided into observation and control groups according to different treatment methods in chemotherapy.The control group was treated with chemotherapy alone.The observation group was treated Shenmai injection combined with chemotherapy.The treatment effect of the two groups was compared,and the incidence of bone marrow blood and side effects before and after treatment were compared.Results.The therapeutic effect of the observation group was 93.55%which was much higher than that of the control group of 74.19%,P<0.05.The bone marrow blood levels of WBC,PLT and Hb in the observation group before and after treatment were 23.97±3.05,6.76±1.27,69.01±8.15,66.96±9.46,91.07±8.15,89.35±7.46,respectively,compared with the control group.The difference in the situation after treatment was found to be significant.The incidence of toxic side effects such as nausea and vomiting,impaired liver function and renal dysfunction in the observation group was 9.68%,which was lower than that of the control group(32.26%,P<0.05).Conclusion.Shemai injection has significant effects on the efficacy and side effects of chemotherapy in acute leukemia and effectively improves the effect of chemotherapy.