景德镇陶瓷文化景观的空间隐喻和权力空间

景德镇陶瓷文化景观空间由多元差异原则构成,呈现出位置关联性,并具备相对独立的逻辑等级体系。本文基于权力空间视角分析了景德镇陶瓷文化景观社会空间位置、权力和行动者位置感知三者之间的关系,揭示了景德镇陶瓷文化景观社会空间中被隐藏的符号权力及其运行过程中所表现出的社会空间与权力之间的相互关系。

血管造影锥形束CT重建算法中的滤波器设计研究

目的:研究血管造影设备锥形束计算机断层摄影(CBCT)在重建三维血管图像算法中的滤波器设计。方法:设计基于Shepp-Logan滤波函数的组合滤波器,包括平滑与截止滤波函数和高频增强滤波函数。采用一种新的组合滤波函数,消除血管图像周边伪影,增强血管边缘,得到更高图像质量的三维血管图像;使用NeuAngio30C血管造影系统进行血管CBCT三维扫描,对扫描数据进行三维重建,滤波函数的参数简明易调节,医生对三维重建结果进行效果评审得到最优参数,并与改进前的滤波器重建结果进行比较。结果:设计的组合滤波器,重建得到的断层图像中血管部分周边伪影较少,边缘清晰、血管完整,三维血管效果良好。结论:所设计的组合滤波函数可起到增强血管的作用,使三维血管成像效果更好,有助于临床诊断与治疗。

基于截断核范数的视频前景与背景分离

为解决鲁棒主成分分析模型(RPCA)中核范数不是矩阵秩函数最佳近似的问题,提出截断核鲁棒主成分分析模型(TNSRPCA)。使用截断核范数替代传统的核范数进行低秩约束,将稀疏部分添加正则化约束克服动态背景产生的扰动,为解决截断核范数使用两步迭代法计算量巨大的问题,提出部分奇异值阈值算子进行求解,提高计算效率。实验结果表明,该模型在视频前景与背景分离中获得了较好的分离效果和抗噪声鲁棒性。

Use Chou’s 5-Steps Rule to Predict Remote Homology Proteins by Merging Grey Incidence Analysis and Domain Similarity Analysis

Detecting remote homology proteins is a challenging problem for both basic research and drug development. Although there are a couple of methods to deal with this problem, the benchmark datasets based on which the existing methods were trained and tested contain many high homologous samples as reflected by the fact that the cutoff threshold was set at 95%. In this study, we reconstructed the benchmark dataset by setting the threshold at 40%, meaning none of the proteins included in the benchmark dataset has more than 40% pairwise sequence identity with any other in the same subset. Using the new benchmark dataset, we proposed a new predictor called “dRHP-GreyFun” based on the grey modeling and functional domain approach. Rigorous cross-validations have indicated that the new predictor is superior to its counterparts in both enhancing success rates and reducing computational cost. The predictor can be downloaded from https://github.com/jcilwz/dRHP-GreyFun.

淮北矿区大断面硬岩巷道炮掘与综掘联合掘进方法

为提高大断面硬岩巷道掘进速度,提出了一种炮掘与综掘联合掘进方法。即精确控制多级掏槽装药量,取消周边眼,全断面一次微差爆破,在巷道中部掘进断面积S≤16.0 m^2的槽腔;然后采用综掘机,扩展该槽腔至巷道设计断面。淮北地区采用该掘进方法,大断面岩巷掘进速度提高约40 m/月,巷道施工速度明显加快。

pLoc-mGpos: Incorporate Key Gene Ontology Information into General PseAAC for Predicting Subcellular Localization of Gram-Positive Bacterial Proteins

The basic unit in life is cell.?It contains many protein molecules located at its different organelles. The growth and reproduction of a cell as well as most of its other biological functions are performed via these proteins. But proteins in different organelles or subcellular locations have different functions. Facing?the avalanche of protein sequences generated in the postgenomic age, we are challenged to develop high throughput tools for identifying the subcellular localization of proteins based on their sequence information alone. Although considerable efforts have been made in this regard, the problem is far apart from being solved yet. Most existing methods can be used to deal with single-location proteins only. Actually, proteins with multi-locations may have some special biological functions that are particularly important for drug targets. Using the ML-GKR (Multi-Label Gaussian Kernel Regression) method,?we developed a new predictor called “pLoc-mGpos” by in-depth extracting the key information from GO (Gene Ontology) into the Chou’s general PseAAC (Pseudo Amino Acid Composition)?for predicting the subcellular localization of Gram-positive bacterial proteins with both single and multiple location sites. Rigorous cross-validation on a same stringent benchmark dataset indicated that the proposed pLoc-mGpos predictor is remarkably superior to “iLoc-Gpos”, the state-of-the-art predictor for the same purpose.?To maximize the convenience of most experimental scientists, a user-friendly web-server for the new powerful predictor has been established at http://www.jci-bioinfo.cn/pLoc-mGpos/, by which users can easily get their desired results without the need to go through the complicated mathematics involved.

Wenxiang: a web-server for drawing wenxiang diagrams

The wenxiang diagram was proposed to represent α-helices in a 2D (two dimensional) space (Chou, K.C., Zhang, C.T., Maggiora, G.M. Proteins: Struct., Funct., Genet., 1997, 28, 99-108). It has the capacity to provide more information in a 2D plane about each of the constituent amino acid residues in an α-helix, and is particularly useful for studying and analyzing amphiphilic helices. To meet the increasing requests for getting the program of generating wenxiang diagrams, a user-friendly web-server called “Wenxiang” has been established. It is accessible to the public at the web-site http://www.jci-bioinfo.cn/wenxiang2 or http://icpr.jci.edu.cn/bioinfo/wenxiang2. Further- more, for the convenience of users, here we provide a step-to-step guide for how to use the Wenxiang web-server to generate the desired wenxiang diagrams.

DR5 is a Suitable System for Studying the Auxin Response in the Poncirus trifoliata-Xanthomonas axonopodis pv.citri Interaction

The local auxin distribution characteristics in the roots,stems,and leaves of stably transformed plantlets of trifoliate orange(Poncirus trifoliata)with auxin reporter system DR5::GUS-YFP were elucidated in this research.The auxin response maxima could be observed in the apex of the root tip,primary phloem of the tender stem,and the margin of the young leaves according to the activity of theβ-glucuronidase(GUS)reporter gene triggered by the auxin responsive DR5 promoter.Auxin responses in the apex of the root tips increased when treated with synthetic auxin 1-naphthylacetic acid(NAA),but decreased when treated with the auxin polar transportation inhibitor 2,3,5-triiodobenzoic acid(TIBA).These results indicated that the DR5 reporter system worked in P.trifoliata for auxin distribution and response observation.Trifoliate orange is highly susceptible to citrus canker disease.Auxin accumulation was observed visually in the invasion sites of the detached leaves inoculated with Xanthomonas axonopodis pv.citri(Xac)by GUS staining;the upregulated expression of the YFP,GH3.1,GH3.9,and SAUR genes assessed by quantitative real-time PCR(qRT-PCR)also identified auxin accumulation in the inoculated tissues following Xac infection.Overall,these findings indicated that the plantlets of P.trifoliata engineered with the auxin reporter gene provided a promising system for studying auxin responses during Xac infection.

Analysis of Common Respiratory Infected Pathogens in 3100 Children after the Coronavirus Disease 2019 Pandemic

Objective To investigate the epidemiological features in children after the coronavirus disease 2019(COVID-19)pandemic.Methods This study collected throat swabs and serum samples from hospitalized pediatric patients of Renmin Hospital of Wuhan University,Wuhan,Hubei province,China before and after the COVID-19 pandemic.Respiratory infected pathogens[adenovirus(ADV),influenza virus A/B(Flu A/B),parainfluenza virus 1/2/3(PIV1/2/3),respiratory syncytial virus(RSV),Mycoplasma pneumoniae(MP),and Chlamydia pneumoniae(CP)]were detected.The pathogens,age,and gender were used to analyze the epidemiological features in children after the COVID-19 pandemic.Results The pathogen detection rate was significantly higher in females than in males(P<0.05),and the infection of PIV1 and MP was mainly manifested.After the COVID-19 pandemic,PIV1,PIV3,RSV,and MP had statistically different detection rates among the age groups(P<0.05),and was mainly detected in patients aged 0–6 years,0–3 years,0–3 years,and 1–6 years,respectively.When comparing before the COVID-19 pandemic,the total detection rate of common respiratory pathogens was lower(P<0.05).Except for the increase in the detection rate of PIV1 and CP,the infection rate of other pathogens had almost decreased.Conclusion The prevention and control measures for the COVID-19 pandemic effectively changed the epidemiological features of common respiratory tract infectious diseases in pediatric children.

长三角区域经济发展差异及时空演变特征分析

区域经济发展差异的原因是复杂多样的,也极具研究意义。文章利用探索性空间数据分析研究长三角区域的经济发展差异与时空动态演变特征。首先,选取长三角地级市的GDP、居民人均收入等指标建立区域经济发展综合评价体系,然后收集2014~2018年各地级市的相关数据并测度经济综合发展程度,最后进行全局和局部空间自相关分析,分析党的十八大后长三角地区经济发展的差异以及动态演变特征。研究结果表明,长三角城市群经济发展表现出显著的全局空间自相关性,空间聚集特征明显,但区域经济在空间分布上存在不平衡。

Discussion on the law of acupoint selection by acupuncture in the treatment of post-stroke depression based on the data mining technique

Objective:To analyze the law of acupoint selection in treatment of depression after stroke by using data mining technique,providing reference for clinical treatment.Methods:Through the retrieval of CNKI,wanfang data resource system and Vip Database,relevant literature on acupuncture treatment of this disease in China from the inception of the database to 2019 were collected.Thus the database of acupoint prescriptions was established.SPSS20.0 and SPSS Modeler 18.0 software were used for statistical analysis to explore the law of acupoint selection.Results:A total of 92 articles were selected,92 acupuncture prescriptions and 88 acupoints with a total frequency of 715 times.The frequency of acupoints of Du channel ranked first.The frequency of acupoints in the head and neck and lower limbs were successively 40.42%(289/715)and 20.46%(172/715).Specific acupoints accounted for 81.82%of the acupoints used,among which confluent acupoints ranked first.The correlation analysis results showed that the highest support of acupoint combination was Baihui(GV20)and Shenting(GV24).Conclusion:The acupoint selection in the treatment of PSD gives priority to the Du channel,following the principle of local acupoint selection,local and remote acupoint selection with the precondition of regulation the mind.Attach importance to the use of specific acupoints,especially the confluent points.

基于灰色理论对临涣煤矿六采区煤层瓦斯压力的预测优化

本文以淮北矿业集团临涣煤矿六采区准备巷道的7煤、9(8)煤和10煤在倾斜方向的实测原始瓦斯压力作为灰色预测模型的原始数据序列,分别利用灰色GM(1,1)模型、新陈代谢GM(1,1)模型、GM(1,1)残差模型和"灰色-马尔科夫(GM(1,1)-Markov)"组合模型进行预测,通过精度等级计算表明:灰色GM(1,1)模型预测精度均为Ⅳ级,预测数据可信度较低;新陈代谢GM(1,1)模型的预测精度分别为Ⅱ级、Ⅲ级和Ⅲ级,预测数据可信度较高;GM(1,1)残差模型的预测精度分别为Ⅲ级、Ⅲ级和Ⅱ级,预测数据可信度较高;GM(1,1)-Markov组合模型的预测精度分别为Ⅱ级、Ⅱ级和Ⅰ级,预测数据可信度高。因此,在矿井其他采区的煤层瓦斯压力实测中,可推荐GM(1,1)-Markov组合模型运用到矿井深部采区的煤层瓦斯压力的预测,以减少瓦斯压力的实测工作,节约大量人力、物力和财力,为矿井防突工作奠定基础。

Self-adaptive rolling motion for snake robots in unstructured environments based on torque control

Snake robots have great potential for exploring and operating in challenging unstructured environments,such as rubble,caves,and narrow pipelines.However,due to the complexity and unpredictability of unstructured environments,designing a controller that can achieve adaptive motion is crucial.This paper proposes a self-adaptive torque-based rolling controller for snake robots,enabling compliant motion in unstructured environments.First,a controller is designed to modify the torque of each motor by focusing on the different motion states of the rolling gait.Second,an experimental platform is established for snake robots to verify the effectiveness of the controller.Finally,a series of rolling experiments are conducted using the torque-based rolling controller.In conclusion,the self-adaptive torque-based rolling controller enhances snake robot adaptability and mobility.

DNA分子“光开关”含钌多吡啶络合物细胞摄取、胞内分布及毒性机理研究

DNA作为遗传信息的载体,了解在细胞核内的组装和结构具有非常重要的意义。目前,使用能够与DNA结合的细胞膜渗透性良好的有机荧光分子作为DNA标记探针是实现这一目标的主要手段。自Barton实验室发现阳离子Ru(Ⅱ)络合物[Ru(bpy)_2(dppz)]^(2+)(bpy=2,2’-联吡啶,dppz=多吡啶并吩嗪)能够作为DNA分子"光开关"以来,d^6八面体多吡啶金属络合物与DNA的结合特性及相关研究,尤其是对这类金属络合物作为高灵敏度和结构特异性DNA探针的研究,便吸引了人们极大的关注。由于这类物质较低的膜渗透性,先前多数研究都只是局限于细胞外,活细胞内DNA直接显像成功的实例较为少见。本课题组发现五氯酚(PCP)和另外两类结构不相关的生化试剂能促进Ru(Ⅱ)多吡啶络合物的细胞特别是细胞核的摄取,[Ru(bpy)_2(dppz)]^(2+)/PCP之间的协同核摄取机理可能是形成了较稳定的亲脂性离子对复合物。Ru(Ⅱ)络合物的两种对映异构体在活细胞内与DNA结合后表现出了明显的手性选择性。这是我们首次发现通过以形成离子对复合物的方式将DNA分子"光开关"Ru(Ⅱ)络合物转入活细胞核内并维持其"光开关"效应,可为研究将其他潜在的具有生物医疗效应的细胞膜不通透金属络合物转入细胞内提供一种全新的方法。

醛肟类神经性毒剂防治药物的新型解毒分子作用机制

吡啶盐醛肟类药物是一类常见的有机磷类神经性毒剂和农药的解毒剂.卤代苯醌是一类致癌性的活性中间体,最近在饮用水消毒副产物中也被检出.我们发现, 2-吡啶盐醛肟类代表性药物如解磷定可快速促进四氯-1,4-苯醌通过连续两步的脱氯水解反应转化为2,5-二氯-3,6-二羟基-1,4-苯醌,比其自然水解的速率提高了18万倍.然而,当解磷定中的氮羟基被掩蔽后则不能促进四氯-1,4-苯醌水解.通过联合运用多种分离和分析鉴定方法发现,解磷定最终转化为其相应的腈类化合物.采用^(18)O-H_2O标记等实验,最终确定2-吡啶盐醛肟类药物对四卤苯醌类致癌物的解毒机理是通过亲核取代同时伴随两次Beckmann裂解反应.本文介绍了这类可在正常生理条件下发生的连续两步Beckmann裂解反应,以期对将来研究这两类具有重要生物学和环境学意义的醛肟类药物及多卤代醌类环境致癌物具有指导作用.

特色小镇产业集聚与都市圈区域集聚的耦合机制研究--以浙江省为例

在构建特色小镇产业集聚与都市圈区域集聚耦合机理的基础上,基于2016~2019年浙江省相关数据,运用耦合评价模型,分析两者间的耦合度、协调度及时间演化特征。结果发现:特色小镇产业集聚度按产业类型存在差异,随时间发展多数稳定;四大都市圈区域集聚度存在横向差异,但总体呈逐年上升趋势;特色小镇与都市圈之间普遍存在一定的耦合关系,不同产业类型特色小镇和不同都市圈之间的协调关系存在明显差异和不同时间演化特征。

Dual drug delivery system with flexible and controllable drug ratios for synergistic chemotherapy

Determination of whether multidrug nanocarriers can deliver and release loaded drugs at a predefined synergistic ratio to target cancer cells is crucial.Although there are many successful applications for delivery of multiple drugs,most current carriers are unable to achieve coordinated loading and release,leading to a drug release ratio that disagrees with the predefined loading ratio.In this work,a simple dual-drug delivery system with a flexible and controllable drug release ratio was constructed to deliver two anticancer drugs,doxorubicin(DOX)and curcumin(CUR).The drug ratio of DOX and CUR can be easily tuned for an enhanced synergistic effect,and the drugs can be released at predesigned ratios due to synchronous drug activation and nanoparticle collapse.Drug release at predefined ratios for synergistic anticancer therapy was demonstrated via in vitro and in vivo experiments.

Ph_3P^+CF_2CO_2^- as an F^- and:CF_2 source for trifluoromethylthiolation of alkyl halides

As trifluoromethylthiolation has received increasing attention recently, many CF_3S-reagents and trifluoromethylthiolation methods have been developed. Herein we describe trifluoromethylthiolation of alkyl halides by using Ph_3 P^+CF_2CO_2 as a fluoride and difluorocarbene source. Difluorocarbene is a versatile intermediate, but its side reactions are usually ignored and the by-products would therefore be discarded. In this work, a side reaction of difluorocarbene, the generation of a fluoride anion from difluorocarbene, was developed into a synthetic tool. Although the trifluoromethylthiolation reaction involved multi-sequential steps, the cleavage of C-F bond, the formation of CF_2=S bond, F-C(S)F_2 bond,and C-SCF_3 bond, the conversion proceeded fast and was completed within 10 min.

Manipulation of optical bound states in the continuum in a metal-dielectric hybrid nanostructure

Optical bound states in the continuum (BICs) are spatially localized states with vanishing radiation, despite their energy embedded in the continuum spectrum of the environment. They are expected to greatly enhance light–matter interaction due to their long lifetime and high quality factor. However, the BICs in all-dielectric structures generally exhibit large mode volumes and their properties are difficult to manipulate. In this paper, we propose a metal–dielectric hybrid nanostructure where a silver film is inserted into the silicon (Si) substrate under the Si nanopillar array. We show that symmetry-protected BIC in this system can couple with surface plasmon polaritons (SPPs) to form a hybridized mode. Compared with previous symmetry-protected BICs in all-dielectric structures, the SPP-coupled BIC has a significantly decreased mode volume, and its corresponding electric field is strongly localized below the Si nanopillars. We also show that the SPP mode makes the original polarizationindependent symmetry-protected BIC become polarization-dependent. In addition, we demonstrate that the silver film in the considered structure can induce a metal mirror effect. The destructive interference between the magnetic dipole inside the Si nanopillars and the mirror magnetic dipole in the silver film can lead to the formation of accidental BICs. Our hybrid structure provides a versatile platform for the manipulation of light–matter interaction in the nanoscale.