Bilayer composite materials of Cu-coated Fe and CuSn10 containing 0%,5%,10%,15%,20%,25%,30%,35%,40%,45%,50%Cu-coated Fe were prepared in mesh belt sintering furnace.Microscopic pore morphology of materials was observe...Bilayer composite materials of Cu-coated Fe and CuSn10 containing 0%,5%,10%,15%,20%,25%,30%,35%,40%,45%,50%Cu-coated Fe were prepared in mesh belt sintering furnace.Microscopic pore morphology of materials was observed,bending strength was tested.Results show that,There is a good bonding between Cu-coated Fe and CuSn10,with the increase of Cu-coated Fe content from 0%to 50%,bending strength of bilayer composite materials increases.展开更多
目的·利用全外显子测序技术(whole-exome sequencing,WES)筛查瘦素-促黑素细胞激素(leptin-melanocyte stimulating hormone,LEP-MSH)通路中的关键基因在肥胖人群中的突变情况。方法·招募2011年1月—2019年7月在上海交通大学...目的·利用全外显子测序技术(whole-exome sequencing,WES)筛查瘦素-促黑素细胞激素(leptin-melanocyte stimulating hormone,LEP-MSH)通路中的关键基因在肥胖人群中的突变情况。方法·招募2011年1月—2019年7月在上海交通大学医学院附属第六人民医院接受袖状胃切除术的119名肥胖患者:年龄17~65岁,体质量指数(body mass index,BMI)≥34 kg/m2。采集研究对象的外周血,提取全基因组DNA并进行全外显子测序,应用生物信息学方法筛选LEP-MSH通路上16个基因(ADCY3、AGRP、BDNF、KSR2、LEP、LEPR、MC3R、MC4R、MCHR1、MRAP2、NTRK2、PCSK1、PHIP、POMC、SH2B1和SIM1)的突变。选取1000 Genomes(1000G)、Exome Aggregation Consortium(ExAC)和Genome Aggregation Database(gnomAD)数据库中总人群最小等位基因频率小于0.02且东亚人群频率小于0.01的罕见变异位点用于后续分析。使用6种突变有害性预测软件来评估变异的损害程度。最后,基于每位患者的临床信息,并根据美国医学遗传学和基因组学学院(America College of Medical Genetics and Genomics,ACMG)指南对所有变异进行致病性判断,仅保留“致病的”“可能致病的”“意义不明的”突变。结果·119名患者中有24名患者检测到了LEP-MSH通路中16个关键基因的26个22种突变,均为杂合突变;其中SH2B1基因突变7个(占总突变数的26.92%),MCHR1基因突变4个(占15.38%),PHIP基因突变3个(占11.53%),ADCY3、LEPR基因突变各2个(各占7.69%),LEP、NTRK2、AGRP、KSR2、MC3R、MC4R、BDNF、PCSK1基因突变各1个(各占3.85%)。3名患者携带SH2B1基因上的相同变异位点,LEPR基因和MCHR1基因上分别有2名患者携带相同变异位点。共有12种变异在3个数据库的东亚人群中都没有被收录,为东亚人群新发变异,分别位于SH2B1(p.V209I、p.R67C、p.L149F)、KSR2(p.P155T)、LEP(p.D106N)、LEPR(p.W132R)、PHIP(p.K1461R)、BDNF(p.N84S)、PCSK1(p.R282W)、NTRK2(p.T732M)、MC3R(p.S71P)和MC4R(p.W174X)。结论·共检测到LEP-MSH通路上22种肥胖可能相关的罕见变异,其中12种为东亚人群新发变异。展开更多
The success of hydraulic fracturing for shale gas recovery has motivated much research interest in understanding the underlying mechanisms (1-3)When hydraulic fractures (HFs) expand, multiple cracks and crack branches...The success of hydraulic fracturing for shale gas recovery has motivated much research interest in understanding the underlying mechanisms (1-3)When hydraulic fractures (HFs) expand, multiple cracks and crack branches can result in complex damage zones and are generally considered to be a stimulated reservoir volume (SRV).展开更多
基金Research project of Heyuan polytechnic(2022ky05,2022ky06)2019 Provincial Science and Technology Special Fund("Big project"+"Task List")project(2019005,2019004).
文摘Bilayer composite materials of Cu-coated Fe and CuSn10 containing 0%,5%,10%,15%,20%,25%,30%,35%,40%,45%,50%Cu-coated Fe were prepared in mesh belt sintering furnace.Microscopic pore morphology of materials was observed,bending strength was tested.Results show that,There is a good bonding between Cu-coated Fe and CuSn10,with the increase of Cu-coated Fe content from 0%to 50%,bending strength of bilayer composite materials increases.
文摘目的·利用全外显子测序技术(whole-exome sequencing,WES)筛查瘦素-促黑素细胞激素(leptin-melanocyte stimulating hormone,LEP-MSH)通路中的关键基因在肥胖人群中的突变情况。方法·招募2011年1月—2019年7月在上海交通大学医学院附属第六人民医院接受袖状胃切除术的119名肥胖患者:年龄17~65岁,体质量指数(body mass index,BMI)≥34 kg/m2。采集研究对象的外周血,提取全基因组DNA并进行全外显子测序,应用生物信息学方法筛选LEP-MSH通路上16个基因(ADCY3、AGRP、BDNF、KSR2、LEP、LEPR、MC3R、MC4R、MCHR1、MRAP2、NTRK2、PCSK1、PHIP、POMC、SH2B1和SIM1)的突变。选取1000 Genomes(1000G)、Exome Aggregation Consortium(ExAC)和Genome Aggregation Database(gnomAD)数据库中总人群最小等位基因频率小于0.02且东亚人群频率小于0.01的罕见变异位点用于后续分析。使用6种突变有害性预测软件来评估变异的损害程度。最后,基于每位患者的临床信息,并根据美国医学遗传学和基因组学学院(America College of Medical Genetics and Genomics,ACMG)指南对所有变异进行致病性判断,仅保留“致病的”“可能致病的”“意义不明的”突变。结果·119名患者中有24名患者检测到了LEP-MSH通路中16个关键基因的26个22种突变,均为杂合突变;其中SH2B1基因突变7个(占总突变数的26.92%),MCHR1基因突变4个(占15.38%),PHIP基因突变3个(占11.53%),ADCY3、LEPR基因突变各2个(各占7.69%),LEP、NTRK2、AGRP、KSR2、MC3R、MC4R、BDNF、PCSK1基因突变各1个(各占3.85%)。3名患者携带SH2B1基因上的相同变异位点,LEPR基因和MCHR1基因上分别有2名患者携带相同变异位点。共有12种变异在3个数据库的东亚人群中都没有被收录,为东亚人群新发变异,分别位于SH2B1(p.V209I、p.R67C、p.L149F)、KSR2(p.P155T)、LEP(p.D106N)、LEPR(p.W132R)、PHIP(p.K1461R)、BDNF(p.N84S)、PCSK1(p.R282W)、NTRK2(p.T732M)、MC3R(p.S71P)和MC4R(p.W174X)。结论·共检测到LEP-MSH通路上22种肥胖可能相关的罕见变异,其中12种为东亚人群新发变异。
基金supported by the National Natural Science Foundation of China(Grant No.11532008)the Special Research Grant for Doctor Discipline by Ministry of Education,China(Grant No.20120002110075)
文摘The success of hydraulic fracturing for shale gas recovery has motivated much research interest in understanding the underlying mechanisms (1-3)When hydraulic fractures (HFs) expand, multiple cracks and crack branches can result in complex damage zones and are generally considered to be a stimulated reservoir volume (SRV).
