The bZIP transcription factor ABSCISIC ACID INSENSITIVE5(ABI5)is a master regulator of seed germination and post-germinative growth in response to abscisic acid(ABA),but the detailed molecularmechanism by which it rep...The bZIP transcription factor ABSCISIC ACID INSENSITIVE5(ABI5)is a master regulator of seed germination and post-germinative growth in response to abscisic acid(ABA),but the detailed molecularmechanism by which it represses plant growth remains unclear.In this study,we used proximity labeling to map the neighboring proteome of ABI5 and identified FCS-LIKE ZINC FINGER PROTEIN 13(FLZ13)as a novel ABI5 interaction partner.Phenotypic analysis of flz13 mutants and FLZ13-overexpressing lines demonstrated that FLZ13 acts as a positive regulator of ABA signaling.Transcriptomic analysis revealed that both FLZ13 and ABI5 downregulate the expression of ABA-repressed and growth-related genes involved in chlorophyll biosynthesis,photosynthesis,and cell wall organization,thereby repressing seed germination and seedling establishment in response to ABA.Further genetic analysis showed that FLZ13 and ABI5 function together to regulate seed germination.Collectively,our findings reveal a previously uncharacterized transcriptional regulatorymechanismby which ABA mediates inhibition of seed germination and seedling establishment.展开更多
基金supported by grants from the Open Competition Program of Top Ten Critical Priorities of Agricultural Science and Technology Innovation for the 14th Five-Year Plan of Guangdong Province(2022SDZG05)the National Natural Science Foundation of China(32270291,32061160467,31870171)to C.G.+7 种基金the Youth Innovation Promotion Association,Chinese Academy of Sciences(2023364)the Guangdong Basic and Applied Basic Research Foundation(2022A1515012319)the Guangzhou Basic and Applied Basic Research Foundation(2023A04J0094)to C.Y.the Sub-Project of Chinese Academy of Sciences Pilot Project(XDA24030502)the Guangdong Provincial Special Fund for Modern Agriculture Industry Technology InnovationTeams(2020KJ148)to Y.W.the National Natural Science Foundation of China(32170362),the Guangdong Natural Science Funds for Distinguished Young Scholars(2022B1515020026)the Youth Innovation Promotion Association,Chinese Academy of Sciences(Y2021094)the Fund of South China Botanical Garden,Chinese Academy of Sciences(QNXM-02)to M.L.
文摘The bZIP transcription factor ABSCISIC ACID INSENSITIVE5(ABI5)is a master regulator of seed germination and post-germinative growth in response to abscisic acid(ABA),but the detailed molecularmechanism by which it represses plant growth remains unclear.In this study,we used proximity labeling to map the neighboring proteome of ABI5 and identified FCS-LIKE ZINC FINGER PROTEIN 13(FLZ13)as a novel ABI5 interaction partner.Phenotypic analysis of flz13 mutants and FLZ13-overexpressing lines demonstrated that FLZ13 acts as a positive regulator of ABA signaling.Transcriptomic analysis revealed that both FLZ13 and ABI5 downregulate the expression of ABA-repressed and growth-related genes involved in chlorophyll biosynthesis,photosynthesis,and cell wall organization,thereby repressing seed germination and seedling establishment in response to ABA.Further genetic analysis showed that FLZ13 and ABI5 function together to regulate seed germination.Collectively,our findings reveal a previously uncharacterized transcriptional regulatorymechanismby which ABA mediates inhibition of seed germination and seedling establishment.
