Some haplotypes of the sucrose synthase gene TaSus1 are associated with thousand-grain weight(TGW)in wheat(Triticum aestivum L.).However,no mutations have been identified within the gene to test this association.The e...Some haplotypes of the sucrose synthase gene TaSus1 are associated with thousand-grain weight(TGW)in wheat(Triticum aestivum L.).However,no mutations have been identified within the gene to test this association.The effects of TaSus1 on grain number per spike(GNS)also are largely unknown.Our previous genome-wide association study identified TaSus-A1 as a candidate gene controlling fertile spikelet number per spike(FSN).In the present study,we generated two independent mutants for the three TaSus1 homoeologs by CRISPR/Cas9-mediated genome editing.The triple mutants displayed lower FSN,GNS,grain number per spikelet(GNST),and TGW than wild-type plants.In 306 hexaploid wheat accessions,two single-nucleotide polymorphisms in TaSus-A1 contributed differently to GNS.Introgression of the two alleles into a wheat genetic background confirmed their effects.The alleles differed in geographical distribution among the accessions.展开更多
Common wheat(Triticum aestivum L.,2n=6x=42,AABBDD)is one of the most widely grown crops worldwide,providing about 20%of the daily calories and protein consumed by humans(Shiferaw et al.,2013).The world population is p...Common wheat(Triticum aestivum L.,2n=6x=42,AABBDD)is one of the most widely grown crops worldwide,providing about 20%of the daily calories and protein consumed by humans(Shiferaw et al.,2013).The world population is projected to reach 9.8 billion in 2050(https://www.un.org/en/desa/world-population-projectedreach-98-billion-2050-and-112-billion-2100);thus,to meet the challenges of global food and nutritional security,wheat yields must continue to be increased through breeding programs and improved agricultural techniques(Hunter et al.,2017).展开更多
基金This work was supported by the Strategic Priority Research Program of Chinese Academy of Sciences(XDA24010104-2).
文摘Some haplotypes of the sucrose synthase gene TaSus1 are associated with thousand-grain weight(TGW)in wheat(Triticum aestivum L.).However,no mutations have been identified within the gene to test this association.The effects of TaSus1 on grain number per spike(GNS)also are largely unknown.Our previous genome-wide association study identified TaSus-A1 as a candidate gene controlling fertile spikelet number per spike(FSN).In the present study,we generated two independent mutants for the three TaSus1 homoeologs by CRISPR/Cas9-mediated genome editing.The triple mutants displayed lower FSN,GNS,grain number per spikelet(GNST),and TGW than wild-type plants.In 306 hexaploid wheat accessions,two single-nucleotide polymorphisms in TaSus-A1 contributed differently to GNS.Introgression of the two alleles into a wheat genetic background confirmed their effects.The alleles differed in geographical distribution among the accessions.
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA24010104-2 and XDA24020201)the National Natural Science Foundation of China(32272122 and 32225038)the National Key Research and Development Program(2022YFF1002904).
文摘Common wheat(Triticum aestivum L.,2n=6x=42,AABBDD)is one of the most widely grown crops worldwide,providing about 20%of the daily calories and protein consumed by humans(Shiferaw et al.,2013).The world population is projected to reach 9.8 billion in 2050(https://www.un.org/en/desa/world-population-projectedreach-98-billion-2050-and-112-billion-2100);thus,to meet the challenges of global food and nutritional security,wheat yields must continue to be increased through breeding programs and improved agricultural techniques(Hunter et al.,2017).
