Objective Vascular endothelial cells senescence is one of major risk factors for atherosclerotic diseases,which can be induced by endogenous peptides,such as angiotensin Ⅱ(Ang Ⅱ).However,the effect of chronic Ang Ⅱ...Objective Vascular endothelial cells senescence is one of major risk factors for atherosclerotic diseases,which can be induced by endogenous peptides,such as angiotensin Ⅱ(Ang Ⅱ).However,the effect of chronic Ang Ⅱ stimulation on endothelial senescence remains unknown.Therefore,this study aims to investigate the changes in morphology and function of human umbilical vein endothelial cells(HUVECs)in response to the chronic stimulation of Ang Ⅱ.展开更多
OBJECTIVE To screen small molecule compounds from traditional Chinese medicine that can enhance recombinant adeno-associated virus(rAAV) transduction.METHODS Recombinant adeno-associated virus(rAAV) has been establish...OBJECTIVE To screen small molecule compounds from traditional Chinese medicine that can enhance recombinant adeno-associated virus(rAAV) transduction.METHODS Recombinant adeno-associated virus(rAAV) has been established as a powerful tool for in vivo gene transfer and achieved much promise in gene therapy applications.However,widespread clinical use has been limited by transduction efficiency.In the current study,we screened a panel of small molecule compound from traditional Chinese medicine focused on AAV intracellular trafficking process and found salvianolic acid B can significantly enhance rAAV2 transduction.RESULTS Salvianolic acid B caused a dose-depen.dent increase in rAAV2 transduction regardless of vector dose,genome architecture,and over a broad range of cell line from various cell type and species(HEK293,HeLa,HepG2,Huh-7,CHO-K1,LO-2).Salvianolic acid B treatment redirected rAAV2 particles toward large vesicles positive for late endosomal(Rab7) and lysosomal(LAMP1) markers.Furthermore,salvianolic acid B acted to increase accumulation of viral particles at the perinuclear region.CONCLUSION In summary,our results suggest that salvi.anolic acid B redirects rAAV2 toward more productive trafficking pathways and stabilizes perinuclear accumulations of vectors,facilitating productive nuclear trafficking.展开更多
Impact–sliding caused by random vibrations between tubes and supports can affect the operation of heat exchangers.In addition,a corrosive environment can cause damage,accelerating the synergism of corrosion and wear....Impact–sliding caused by random vibrations between tubes and supports can affect the operation of heat exchangers.In addition,a corrosive environment can cause damage,accelerating the synergism of corrosion and wear.Therefore,the focus of this work was the impact–sliding fretting tribocorrosion behavior of 316L heat exchanger tubes at different halide concentrations.A device system incorporating the in situ electrochemical measurements of impact–sliding fretting corrosion wear was constructed,and experiments on 316L heat exchanger tubes in sodium chloride(NaCl)solution with different concentrations(0.0,0.1,0.5,1.0,3.5,and 5.0 wt%)were carried out.The synergism between wear and corrosion was also calculated and analyzed.The wear and damage mechanisms were elucidated by correlating the corrosion–wear synergism,morphologies,and material loss rates.The results indicated that the stable wear stage occurred at approximately 9–12 h,after which the corrosion current increased with the expansion of the wear area.As the halide concentration increased,the scale of damage on the wear scars gradually decreased,changing from being dominated by cracks,delaminations,and grooves to being dominated by scratches,microgrooves,and holes.There was an obvious positive synergism between wear and corrosion.The material loss was dominated by pure mechanical wear and wear enhanced by corrosion,but corrosion enhanced by wear contributed more than tangential sliding fretting corrosion.The total mass loss increased gradually in the range of 0.0–0.5 wt%and decreased in the range of 0.5–5.0 wt%.Large-scale damage enhanced by corrosivity and small-scale damage reduced by lubricity dominated the material loss at low and high concentrations,respectively.展开更多
文摘Objective Vascular endothelial cells senescence is one of major risk factors for atherosclerotic diseases,which can be induced by endogenous peptides,such as angiotensin Ⅱ(Ang Ⅱ).However,the effect of chronic Ang Ⅱ stimulation on endothelial senescence remains unknown.Therefore,this study aims to investigate the changes in morphology and function of human umbilical vein endothelial cells(HUVECs)in response to the chronic stimulation of Ang Ⅱ.
基金supported by National Natural Science Foundation of China (81371669 81271691)
文摘OBJECTIVE To screen small molecule compounds from traditional Chinese medicine that can enhance recombinant adeno-associated virus(rAAV) transduction.METHODS Recombinant adeno-associated virus(rAAV) has been established as a powerful tool for in vivo gene transfer and achieved much promise in gene therapy applications.However,widespread clinical use has been limited by transduction efficiency.In the current study,we screened a panel of small molecule compound from traditional Chinese medicine focused on AAV intracellular trafficking process and found salvianolic acid B can significantly enhance rAAV2 transduction.RESULTS Salvianolic acid B caused a dose-depen.dent increase in rAAV2 transduction regardless of vector dose,genome architecture,and over a broad range of cell line from various cell type and species(HEK293,HeLa,HepG2,Huh-7,CHO-K1,LO-2).Salvianolic acid B treatment redirected rAAV2 particles toward large vesicles positive for late endosomal(Rab7) and lysosomal(LAMP1) markers.Furthermore,salvianolic acid B acted to increase accumulation of viral particles at the perinuclear region.CONCLUSION In summary,our results suggest that salvi.anolic acid B redirects rAAV2 toward more productive trafficking pathways and stabilizes perinuclear accumulations of vectors,facilitating productive nuclear trafficking.
基金The work was funded by the Materials Ageing Institute.Thanks to Dr.Kai GUO from School of Environmental and Chemical Engineering,Yanshan University,China,for his technical support.
文摘Impact–sliding caused by random vibrations between tubes and supports can affect the operation of heat exchangers.In addition,a corrosive environment can cause damage,accelerating the synergism of corrosion and wear.Therefore,the focus of this work was the impact–sliding fretting tribocorrosion behavior of 316L heat exchanger tubes at different halide concentrations.A device system incorporating the in situ electrochemical measurements of impact–sliding fretting corrosion wear was constructed,and experiments on 316L heat exchanger tubes in sodium chloride(NaCl)solution with different concentrations(0.0,0.1,0.5,1.0,3.5,and 5.0 wt%)were carried out.The synergism between wear and corrosion was also calculated and analyzed.The wear and damage mechanisms were elucidated by correlating the corrosion–wear synergism,morphologies,and material loss rates.The results indicated that the stable wear stage occurred at approximately 9–12 h,after which the corrosion current increased with the expansion of the wear area.As the halide concentration increased,the scale of damage on the wear scars gradually decreased,changing from being dominated by cracks,delaminations,and grooves to being dominated by scratches,microgrooves,and holes.There was an obvious positive synergism between wear and corrosion.The material loss was dominated by pure mechanical wear and wear enhanced by corrosion,but corrosion enhanced by wear contributed more than tangential sliding fretting corrosion.The total mass loss increased gradually in the range of 0.0–0.5 wt%and decreased in the range of 0.5–5.0 wt%.Large-scale damage enhanced by corrosivity and small-scale damage reduced by lubricity dominated the material loss at low and high concentrations,respectively.
