简化基因组测序评价高邮鸭保种效果的研究

研究旨在评价高邮鸭原种场的保种效果,为高邮鸭保种提供理论依据。利用简化基因组测序方法ddRAD鉴定高邮鸭原始群体(GS)和原种场群体(GC)的SNP标记,比较分析两个群体的遗传多样性差异,并使用遗传分化指数(F_(ST))和核苷酸多样性比值(π ratio)比率联合筛选选择信号区域及其相关基因,对得到的受选基因进行GO和KEGG功能富集分析。结果显示:与原始群体相比,原种场群体遗传杂合度保持不变,近交系数有所下降;原种场群体与环境应激和甲基化调控相关的基因受到了一定程度的选择。结果表明高邮鸭原种场群体保种效果较好,可以继续沿用现有的小群保种方法。

Semen Quality Assessment of Suyou Ⅰ Duck

[Objective] This paper was to establish a method for quality assessment of Suyou Ⅰ duck. [Method] Semen was collected from 20 healthy drakes using back massage method,to determine semen volume,sperm motility,sperm density,sperm morphology and semen quality factor( SQF) of each individual.[Result]Semen was successfully collected from 15 out of 20 ducks. The results of semen quality assessment showed that the semen volume,sperm density,sperm motility,normal morphology ratio and SQF were( 0. 17 ± 0. 05) mL,( 25. 52 ± 4. 73) × 10~8 sperm/mL,( 0. 86 ± 0. 10),( 86. 93 ± 2. 13) % and( 387. 5 ±129. 25),respectively. The abnormal sperm ratio was( 13. 07 ±2. 13) %,among which the percentages of bent sperm,huge head sperm and damaged sperm were( 7. 70 ± 1. 97) %,( 2. 53 ± 1. 06) % and( 2. 83 ± 0. 79) %,respectively. [Conclusion]Semen quality of Suyou Ⅰ duck is generally high,and could be applied in the production and breeding.

鹅细小病毒研究进展

小鹅瘟是由鹅细小病毒(Goose parvovirus,GPV)感染引起的一种急性、接触性、败血性传染病,由于传染性强、发病率和死亡率高,严重威胁了水禽养殖业的健康持续发展。GPV遗传稳定性高,但点突变的累积和基因型间的重组极易产生新的水禽细小病毒,因此近年来大中小型鹅场的新型鹅细小病毒发病率频增;同时,水禽病原的复杂性导致必须依赖实验室诊断结果来鉴别GPV感染。缺乏对GPV感染的流行病学认知,尤其是缺少准确的实验室诊断方法,可能给养殖户和企业带来重大的经济损失。为深入了解GPV,文章对GPV的流行病学、病原学、基因组演化和实验室诊断技术四个方面进行综述,旨在为该病原的基础研究和实验室诊断提供参考。