Grain size is one of key agronomic traits associated with grain yield and grain quality. Both major quantitative trait loci GS3 and GL3.1 play a predominant role in negative regulation of grain size. In this study, a ...Grain size is one of key agronomic traits associated with grain yield and grain quality. Both major quantitative trait loci GS3 and GL3.1 play a predominant role in negative regulation of grain size. In this study, a CRISPR/Cas9-mediated multiplex genome editing system was used to simultaneously edit GS3 and GL3.1 in a typical japonica rice Nipponbare. In T1 generation, we found that gs3 formed slender grain with lower chalkiness percentage, while gs3gl3.1 produced larger grain with higher chalkiness percentage. In terms of other agronomic traits, flag leaf size, grain number and grain yield of both gs3 and gs3gl3.1 mutants were affected. It is noteworthy that gs3 and gs3gl3.1 mutants both led to dramatical reduction of grain number, thereby decreased grain yield. In conclusion, these results indicated that knockout of GS3 and GL3.1 could rapidly improve grain size, but probably have some negative influences on grain quality and grain yield.展开更多
Grain size is a major determinant of grain weight, which is one of the components of rice yield. The objective o this study was to identify novel, and important quantitative trait loci(QTLs) for grain size and weight ...Grain size is a major determinant of grain weight, which is one of the components of rice yield. The objective o this study was to identify novel, and important quantitative trait loci(QTLs) for grain size and weight in rice. QTLs were mapped using a BC4F4 population including 192 backcross inbred lines(BILs) derived from a backcross between Xiaolijing(XLJ) and recombinant inbred lines(RILs). The mapping population was planted in both Lingshui(Hainan, 2015) and Fuyang(Zhejiang, 2016), with the short-and long-day conditions, respectively. A total of 10 QTLs for grain length, four for grain width, four for the ratio of grain length to width, and 11 for grain weight were detected in at least one environment and were distributed across 11 chromosomes. The phenotypic variance explained ranged from 6.76–25.68%, 14.30–34.03%, 5.28–26.50%, and 3.01–22.87% for grain length, grain width, the ratio of grain length to width, and thousand grain weight, respectively. Using the sequential residual heterozygotes(SeqRHs) method, qGS7.1, a QTL for grain size and weight, was mapped in a 3.2-Mb interval on chromosome 7. No QTLs about grain size and weight were reported in previous studies in this region, providing a good candidate for functional analysis and breeding utilization.展开更多
Many excellent genes in wild rice have been lost during the domestication of wild rice to cultivated rice.In this study,introgression lines(ILs)were produced with a wild rice(Oryza rufipogon)accession,BJ194,as a donor...Many excellent genes in wild rice have been lost during the domestication of wild rice to cultivated rice.In this study,introgression lines(ILs)were produced with a wild rice(Oryza rufipogon)accession,BJ194,as a donor parent and an indica restorer line,Zhonghui 8015(ZH8015),as a recipient parent to map QTLs for plant height.We identified four QTLs(qPH3.1,qPH3.2,qPH2,and qPH8)related to plant height distributed on chromosomes 2,3 and 8.Furthermore,we sequenced and analyzed qPH3.2 located in the interval of RM15753-RM3525,and found this QTL may be a new locus regulating rice plant height.展开更多
To investigate the genetic bases of grain weight(GW),a set of 76 chromosome segment substitution lines(CSSLs,BC4F5)were developed from the cross between Xieqingzao B(XQZB)and Zhonghui 9308(ZH9308)using a marker-assist...To investigate the genetic bases of grain weight(GW),a set of 76 chromosome segment substitution lines(CSSLs,BC4F5)were developed from the cross between Xieqingzao B(XQZB)and Zhonghui 9308(ZH9308)using a marker-assisted selection(MAS).All substituted segments represented by 120 markers in those lines covered 96.7%of the donor parent(ZH9308).Consequently,two QTLs(qTGW1 and qTGW6)for 1000-grain weight(TGW)were preliminarily mapped on chromosomes 1 and 6,respectively.The line CSSL7 was selected for further mapping of qGL1.4/qTGW1.As a result,qGL1.4 was validated and narrowed to a 1.4 Mb interval between markers InDel15 and RM11872 using secondary F2,and narrowed to a 500 kb in F2:3 populations between markers RM11824 and RM11842.In F4:5 secondary population,using homozygous recombinant strategy,qGL1.4 was finally fine-mapped to a 286.4 kb region between markers D-12 and TG-57.In addition,a near-isogenic line(NIL)harbouring qGL1.4 was developed using MAS approach,which showed enhanced grain length compared with ZH9308 without changing other traits.In summary,these results lay a foundation for the genetic isolation of qGL1.4 and molecular breeding in rice.展开更多
基金supported by the National Nature Science Foundation of China(Grant Nos.31521064 and 31961143016)Technological Innovation Project of Chinese Academy of Agricultural Sciences(Grant No.CAASASTIP-2013-CNRRI)+2 种基金Major Transgenic Projects of China(Grant No.2016ZX08001-002)National Key Research and Development Program(Grant No.2016YFD0101801)the Natural Science Foundation of Innovation Research Group(Grant No.31521064)。
文摘Grain size is one of key agronomic traits associated with grain yield and grain quality. Both major quantitative trait loci GS3 and GL3.1 play a predominant role in negative regulation of grain size. In this study, a CRISPR/Cas9-mediated multiplex genome editing system was used to simultaneously edit GS3 and GL3.1 in a typical japonica rice Nipponbare. In T1 generation, we found that gs3 formed slender grain with lower chalkiness percentage, while gs3gl3.1 produced larger grain with higher chalkiness percentage. In terms of other agronomic traits, flag leaf size, grain number and grain yield of both gs3 and gs3gl3.1 mutants were affected. It is noteworthy that gs3 and gs3gl3.1 mutants both led to dramatical reduction of grain number, thereby decreased grain yield. In conclusion, these results indicated that knockout of GS3 and GL3.1 could rapidly improve grain size, but probably have some negative influences on grain quality and grain yield.
基金supported by grants from the National Key Research and Development Program of China (2018YFD0100806)the Zhejiang Provincial Natural Science Foundation of China (LY18C130008)+2 种基金the National Natural Science Foundation of China (31521064)the Major Project of the Genetically Modified and National Key Transgenic Research Projects, China (2016ZX08001-002)the Super Rice Breeding Innovation Team and Rice Heterosis Mechanism Research Innovation Team of the Chinese Academy of Agricultural Sciences Innovation Project (CAASASTIP-2013-CNRRI)
文摘Grain size is a major determinant of grain weight, which is one of the components of rice yield. The objective o this study was to identify novel, and important quantitative trait loci(QTLs) for grain size and weight in rice. QTLs were mapped using a BC4F4 population including 192 backcross inbred lines(BILs) derived from a backcross between Xiaolijing(XLJ) and recombinant inbred lines(RILs). The mapping population was planted in both Lingshui(Hainan, 2015) and Fuyang(Zhejiang, 2016), with the short-and long-day conditions, respectively. A total of 10 QTLs for grain length, four for grain width, four for the ratio of grain length to width, and 11 for grain weight were detected in at least one environment and were distributed across 11 chromosomes. The phenotypic variance explained ranged from 6.76–25.68%, 14.30–34.03%, 5.28–26.50%, and 3.01–22.87% for grain length, grain width, the ratio of grain length to width, and thousand grain weight, respectively. Using the sequential residual heterozygotes(SeqRHs) method, qGS7.1, a QTL for grain size and weight, was mapped in a 3.2-Mb interval on chromosome 7. No QTLs about grain size and weight were reported in previous studies in this region, providing a good candidate for functional analysis and breeding utilization.
基金supported by the National Natural Science Foundation of China (Grant No. 31961143016)the National Rice Industry Technology System of China (Grant No.CARS-01-03)+2 种基金the ‘14th Five-Year Plan’ Major Special Projects for Breeding New Rice Varieties of Zhejiang Province, China (Grant No. 2021C02063-1)the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (Grant No. CAAS-ASTIP2013-CNRRI)Hainan Yazhou Bay Seed Laboratory Oratory, China (Grant No. B21HJ0219)
文摘Many excellent genes in wild rice have been lost during the domestication of wild rice to cultivated rice.In this study,introgression lines(ILs)were produced with a wild rice(Oryza rufipogon)accession,BJ194,as a donor parent and an indica restorer line,Zhonghui 8015(ZH8015),as a recipient parent to map QTLs for plant height.We identified four QTLs(qPH3.1,qPH3.2,qPH2,and qPH8)related to plant height distributed on chromosomes 2,3 and 8.Furthermore,we sequenced and analyzed qPH3.2 located in the interval of RM15753-RM3525,and found this QTL may be a new locus regulating rice plant height.
基金supported by the Natural Science Foundation of China(Grant No.31961143016)the National Key Research and Development Program(Grant No.2016YFD0101801)+1 种基金the Fundamental Research Funds of Central Public Welfare Research Institutions(Grant No.CPSIBRF-CNRRI-202102)the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Science(Grant No.CAAS-ASTIP-2013-CNRRI).
文摘To investigate the genetic bases of grain weight(GW),a set of 76 chromosome segment substitution lines(CSSLs,BC4F5)were developed from the cross between Xieqingzao B(XQZB)and Zhonghui 9308(ZH9308)using a marker-assisted selection(MAS).All substituted segments represented by 120 markers in those lines covered 96.7%of the donor parent(ZH9308).Consequently,two QTLs(qTGW1 and qTGW6)for 1000-grain weight(TGW)were preliminarily mapped on chromosomes 1 and 6,respectively.The line CSSL7 was selected for further mapping of qGL1.4/qTGW1.As a result,qGL1.4 was validated and narrowed to a 1.4 Mb interval between markers InDel15 and RM11872 using secondary F2,and narrowed to a 500 kb in F2:3 populations between markers RM11824 and RM11842.In F4:5 secondary population,using homozygous recombinant strategy,qGL1.4 was finally fine-mapped to a 286.4 kb region between markers D-12 and TG-57.In addition,a near-isogenic line(NIL)harbouring qGL1.4 was developed using MAS approach,which showed enhanced grain length compared with ZH9308 without changing other traits.In summary,these results lay a foundation for the genetic isolation of qGL1.4 and molecular breeding in rice.
