Comparison of the conventional tube and erythrocyte-magnetized technology in titration of red blood cell alloantibodies

BACKGROUND Erythrocyte alloantibodies are mainly produced after immune stimulation,such as blood transfusion,pregnancy,and transplantation,and are the leading causes of severe hemolytic transfusion reactions and difficulty in blood grouping and matching.Therefore,antibody screening is critical to prevent and improve red cell alloantibodies.Routine tube assay is the primary detection method of antibody screening.Recently,erythrocyte-magnetized technology(EMT)has been increasingly used in clinical practice.This study intends to probe the application and efficacy of the conventional tube and EMT in red blood cell alloantibody titration to provide a reference for clinical blood transfusion.AIM To investigate the application value of conventional tube and EMT in red blood cell alloantibody titration and enhance the safety of blood transfusion practice.METHODS A total of 1298 blood samples were harvested from blood donors at the Department of Blood Transfusion of our hospital from March 2021 to December 2022.A 5 mL blood sample was collected in tubing,which was then cut,and the whole blood was put into a test tube for centrifugation to separate the serum.Different red blood cell blood group antibody titers were simultaneously detected using the tube polybrene test,tube antiglobulin test(AGT),and EMT screening irregular antibody methods to determine the best test method.RESULTS Simultaneous detection was performed through the tube polybrene test,tube AGT and EMT screening irregular antibodies.It was discovered that the EMT screening irregular antibody method could detect all immunoglobulin G(IgG)and immunoglobulin M(IgM)irregular antibodies,and the results of manual tube AGT were satisfactory,but the operation time was lengthy,and the equipment had a large footprint.The EMT screening irregular antibody assay was also conducted to determine its activity against type O Rh(D)red blood cells,and the outcomes were satisfactory.Furthermore,compared to the conventional tube method,the EMT screening irregular antibody method was more cost-effective and had significantly higher detection efficiency.CONCLUSION With a higher detection rate,the EMT screening irregular antibody method can detect both IgG and IgM irregular antibodies faster and more effectively than the conventional tube method.

Ultrasound evaluation of fetal left ventricular Tei index in gestational hypertension and its correlation with the degree of placental hypoxia

Objective:To determine the fetal left ventricular Tei index level in gestational hypertension, and evaluate its correlation with the degree of placental hypoxia.Methods: A total of 98 pregnant women with gestational hypertension who gave birth in our hospital between May 2013 and May 2016 were selected as the gestational hypertension group and 50 normal pregnant women were selected as the normal pregnancy group. Before delivery, the color Doppler diasonograph was used to detect fetal left ventricular Tei index and umbilical blood flow parameters, enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of hypoxia-related indexes and nerve injury indexes in umbilical venous blood after delivery of baby, and Pearson test was used to further evaluate the internal relations between fetal left ventricular Tei index and placental hypoxia degree.Results: Fetal left ventricular Tei index level of gestational hypertension group was higher than that of normal pregnancy group. Fetal ultrasonic umbilical blood flow parameters RI, PI and S/D levels of gestational hypertension group were higher than those of normal pregnancy group, ischemia hypoxia indexes HIF-1α and HSP70 contents in umbilical venous blood were higher than those of normal pregnancy group while NO content was lower than that of normal pregnancy group, and nerve injury indexes NSE, S100B and MBP contents in umbilical venous blood were higher than those of normal pregnancy group. After Pearson test, fetal left ventricular Tei index in gestational hypertension was directly correlated with umbilical blood flow parameter levels as well as ischemia hypoxia index and nerve injury index contents in umbilical venous blood. Conclusion:Fetal left ventricular Tei index level in gestational hypertension is higher than that in normal fetus, and Tei index level is directly correlated with placental ischemia hypoxia degree.