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葛根素通过上调Nrf2通路缓解小鼠非酒精性脂肪性肝炎
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作者 钟雪晴 李国栋 +1 位作者 王可洋 徐阳 《世界华人消化杂志》 CAS 2023年第9期352-360,共9页
背景非酒精性脂肪性肝炎(nonalcoholic steatohepatitis,NASH)发病率呈上升趋势,炎症和氧化应激是该疾病进展的关键.葛根素有抗炎和抗氧化的活性,葛根素对小鼠非酒精性脂肪性肝炎的作用及其机制尚无定论.目的探讨葛根素对非酒精性脂肪... 背景非酒精性脂肪性肝炎(nonalcoholic steatohepatitis,NASH)发病率呈上升趋势,炎症和氧化应激是该疾病进展的关键.葛根素有抗炎和抗氧化的活性,葛根素对小鼠非酒精性脂肪性肝炎的作用及其机制尚无定论.目的探讨葛根素对非酒精性脂肪性肝炎的改善效应及其机制.方法采用蛋氨酸胆碱缺乏饮食构造小鼠非酒精性脂肪性肝炎模型.C57BL/6小鼠随机分为正常饮食组,模型组,葛根素组.三种饮食分别予以正常饮食、蛋氨酸胆碱缺乏饮食、蛋氨酸胆碱缺乏饮食加葛根素喂养4 wk;收集血清和肝脏标本以检测组织学变化、炎症指标、肝功能、氧化应激相关指标.结果与正常组比较,模型组小鼠肝脏甘油三酯和胆固醇均有升高,H&E染色结果显示肝脏出现明显脂肪变性和炎症反应以及纤维化.与正常组相比,模型组抗氧化因子谷胱甘肽和超氧化物歧化酶活性显著降低,脂质过氧化标志物丙二醛是显著升高的.与模型组比较,葛根素组小鼠肝脏中甘油三酯和胆固醇明显降低(P<0.05),炎症因子如肿瘤坏死因子α及白介素1β明显降低(P<0.05),小鼠肝脏中纤维化基因αSMA、PAI-1、COL1A1、TGFβ表达明显降低(P<0.05),血清谷丙转氨酶和谷草转氨酶明显降低(P<0.05).与模型组相比,葛根素组中谷胱甘肽和超氧化物歧化酶活性显著升高,丙二醛显著降低,细胞核内转录因子NF-E2相关因子2(NF-E2-related factor 2,Nrf2)及其靶基因血红素加氧酶-1(hemeoxygenase-1,HO-1)表达是升高的(P<0.05).结论葛根素对蛋氨酸胆碱缺乏饮食诱导的非酒精性脂肪性肝炎有显著改善作用,其机制可能与其抑制炎症、抑制纤维化以及上调Nrf2通路有关.本研究为深入了解NASH的发病机制提供了新的思路,并为葛根素作为抗NASH的潜在药物提供了进一步的证据. 展开更多
关键词 非酒精性脂肪性肝病 葛根素 NRF2 氧化应激
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Successful management of life-threatening aortoesophageal fistula: A case report and review of the literature
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作者 xue-qing zhong Guo-Xiong Li 《World Journal of Clinical Cases》 SCIE 2022年第12期3814-3821,共8页
BACKGROUND Aortoesophageal fistula(AEF)is a rare but life-threatening cause of upper gastrointestinal bleeding.Only a handful of cases of successful management of AEF caused by esophageal cancer have been reported.The... BACKGROUND Aortoesophageal fistula(AEF)is a rare but life-threatening cause of upper gastrointestinal bleeding.Only a handful of cases of successful management of AEF caused by esophageal cancer have been reported.The purpose of this study is to report a case of AEF managed by endovascular aortic repair and review the relevant literature.CASE SUMMARY A 66-year-old man with upper gastroenterology bleeding presented at the Emergency Department of our hospital complaining of chest pain,fever and hematemesis for 6 h.He had vomited 400 mL of bright-red blood and experienced severe chest pain 6 h prior.He had a past medical history of advanced esophageal cancer.He received chemoradiotherapy but stopped 8 mo prior because of intolerance.A chest contrast computed tomographic scan revealed communication between the esophagus and the descending aorta as well as a descending aortic pseudoaneurysm.According to the symptoms and imaging findings,AEF was our primary consideration.The patient underwent aortic angiography,which indicated AEF and descending aortic pseudoaneurysm.Emergency percutaneous thoracic endovascular aortic repair(TEVAR)of the descending aorta was performed,and bleeding was controlled after TEVAR.He received antibiotics and was discharged after treatment.However,he died 2 mo after the TEVAR due to cancer progression.CONCLUSION Although AEF is a lethal condition,timely diagnosis and TEVAR may successfully control bleeding. 展开更多
关键词 Upper gastrointestinal bleeding Aortoesophageal fistula Thoracic endovascular aortic repair Esophageal cancer Chest pain Case report
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