Objective: To investigate the expression of miRNA21 in hippocampus of rats with pentylenetetrazol-induced epilepsy and its possible mechanism. Methods: Twenty-four healthy male wister rats was randomly divided into tw...Objective: To investigate the expression of miRNA21 in hippocampus of rats with pentylenetetrazol-induced epilepsy and its possible mechanism. Methods: Twenty-four healthy male wister rats was randomly divided into two groups, the normal group and the epilepsy group. The Racine grade scores of the two groups of rats were recorded to evaluate the establishment of a rat model of epilepsy. The differentially expressed of microRNAs targeting bcl-2 in the hippocampus of epileptic rats was screened by bioinformatics method. The expression of microRNA21 in hippocampus of epileptic rats was verified by qRT-PCR. Western blot and immunohistochemistry were used to detect the expression of Bcl-2 in rat hippocampus;Hoechst 33258 was used to detect the apoptosis of rat hippocampal neurons. Results: The Racine score of the rats in the epilepsy group was significantly higher than that in the normal control group (P<0.01). Compared with the normal group, the expression of miRNA21 in the hippocampus of the epileptic group increased (P<0.01) and the expression of Bcl-2 decreased (P<0.01). Conclusion: Epilepsy can up-regulate the expression of miRNA21 in rat hippocampal neurons, and it may further induce apoptosis of hippocampal neurons by down-regulating Bcl-2, which may affect epileptic rats.展开更多
文摘Objective: To investigate the expression of miRNA21 in hippocampus of rats with pentylenetetrazol-induced epilepsy and its possible mechanism. Methods: Twenty-four healthy male wister rats was randomly divided into two groups, the normal group and the epilepsy group. The Racine grade scores of the two groups of rats were recorded to evaluate the establishment of a rat model of epilepsy. The differentially expressed of microRNAs targeting bcl-2 in the hippocampus of epileptic rats was screened by bioinformatics method. The expression of microRNA21 in hippocampus of epileptic rats was verified by qRT-PCR. Western blot and immunohistochemistry were used to detect the expression of Bcl-2 in rat hippocampus;Hoechst 33258 was used to detect the apoptosis of rat hippocampal neurons. Results: The Racine score of the rats in the epilepsy group was significantly higher than that in the normal control group (P<0.01). Compared with the normal group, the expression of miRNA21 in the hippocampus of the epileptic group increased (P<0.01) and the expression of Bcl-2 decreased (P<0.01). Conclusion: Epilepsy can up-regulate the expression of miRNA21 in rat hippocampal neurons, and it may further induce apoptosis of hippocampal neurons by down-regulating Bcl-2, which may affect epileptic rats.
