Different Regulation Effects of Gekko Sulfated Glycopeptide in Breast and Liver Cancer Cell Lines

Background:Our previous work demonstrated that Gekko sulfated glycopeptide extracted from the Chinese gecko Shou gong(Gekko swinhonis Guenther)could inhibit tumor growth by regulating basic fibroblast growth factor.However,basic fibroblast growth factor has opposing effects on growth in breast and liver cancers.Direct effects and mechanisms of Gekko sulfated glycopeptide on tumor growth remain unclear and are ripe for further exploration.Methods:Differential regulation by Gekko sulfated glycopeptide and bFGF were studied in established human breast cancer MCF-7 cells and hepatocarcinoma HepG2 cells.Cell proliferation was evaluated with a Trypan blue exclusion assay.Cell cycle phases were measured by flow cytometry.Basic fibroblast growth factor,transforming growth factor-β1,and p21WAF1/CIP1 mRNA and protein expression levels were detected by real-time PCR(mRNA)and ELISA(protein).Changes in phosphorylated extracellular signal-regulated kinase levels were analyzed by Western blot.Results:Data indicated that Gekko sulfated glycopeptide inhibited the proliferation of HepG2 cells(P<0.001)and also blocked basic fibroblast growth factor-stimulated proliferation of these cells(P=0.001).Gekko sulfated glycopeptide was shown to increase transforming growth factor-β1 and p21WAF1/CiP1 expression(P<0.01)and partially compensate for reductions therein induced by basic fibroblast growth factor.Conversely,in MCF-7 cells,Gekko sulfated glycopeptide alone had no observable effect on transforming growth factor-β1 and p21WAF1/CiP1 expression.Gekko sulfated glycopeptide did,however,enhance basic fibroblast growth factor-induced inhibition of cell proliferation and transforming growth factor-β1 and p21WAF1/CiP1 expression in the MCF-7 cells(P=0.001,P<0.01,P<0.01,respectively).Gekko sulfated glycopeptide was shown to suppress basic fibroblast growth factor secretion in both HepG2 and MCF-7 cell lines(P<0.05 and P<0.01,respectively)and inhibited extracellular signal-regulated kinase 1/2 phosphorylation facilitated by basic fibroblast growth factor.Gekko sulfated glycopeptide alone decreased phosphorylated extracellular signal-regulated kinase in HepG2 cells but did not visibly affect phosphorylated extracellular signal-regulated kinase levels in MCF-7 cells.Conclusions:Gekko sulfated glycopeptide,a basic fibroblast growth factor inhibitor,differentially regulates growth in different cancer cell lines,and these differences may be determined by the opposing effects of basic fibroblast growth factor on transforming growth factor-β1 and p21WAF1/CiP1 levels in breast and liver cancer cells.

A meta-analysis of the treatment of cancer-related insomnia with acupuncture

To systematically evaluate the clinical efficacy and safety of acupuncture in treating cancer-related insomnia.Methods:Randomized controlled trials(RCTs)and quasi-randomized controlled trials of acupuncture in the treatment of cancer-related insomnia were searched by computer in a Pubmed database,and the retrieved literature was screened according to inclusion and exclusion criteria.After the literature screening was completed,two reviewers read the literature titles independently and extracted effective information.The migration risk assessment was performed by Cochrane.All statistical calculation requirements for this study were fully completed by RevMan5.3 software.Results:Among the 68 selected works of literature,3 RCTs were finally included,with a total of 133 patients.The risk of research bias is generally very low.In 3 RCTs,the sleep score of the experimental group was decreased by 0.48 more than that of the control group in 3 RCTs,with a 95%confidence interval of-0.82 and-0.13,and the degree of decrease was statistically significant(Z=2.70,P<0.05).Conclusion:Higher levels may indicate that acupuncture-related therapies are more effective in improving cancer-related insomnia.Although the included literatures feature low bias and no heterogeneity,the number may have reduced its clinical significance.Therefore,more studies on high-quality randomized controlled trials should be conducted in the future to better clarify the therapeutic effect of acupuncture and moxibustion on cancer-related insomnia.