DJ-1 Alters Epirubicin-induced Apoptosis via Modulating Epirubicin-activated Autophagy in Human Gastric Cancer Cells

Epirubicin,which is a conventional chemotherapeutic drug for gastric cancer,has innate and adaptive chemoresistance.Recent studies revealed that epirubicin could induce autophagy as a defensive mechanism in drug resistance of mammary carcinoma.Another study implied that D J-1 may be a chemoresistance-related gene.But the association between D J-1 and drug resistance of epirubicin in gastric cancer is still ambiguous.In the present report,we explored whether and how D J-1 conduced to epirubicin-induced apoptosis in gastric cancer.Epirubicin dose-dependently increased the expression of DJ-1 and induced autophagy.Knockdown of DJ-1 notably enhanced epirubicin-induced cell apoptosis,whereas overexpression of DJ-1 attenuated epirubicin-induced cell apoptosis.Further studies revealed that down-regulation of DJ-1 modulated epirubicin-activated autophagy which augmented epirubicin-induced apoptosis.In conclusion,our results validated that DJ-1 reduced epirubicin-induced apoptosis in gastric cancer cells via modulating epirubicin-activated autophagy.

Expression of Stanniocalcin 2 in Breast Cancer and Its Clinical Sigmiicance

This study aims to explore the expression of stanniocalcin 2(STC2)gene in breast cancer and its clinical significance.Female patients with breast cancer from Zhongnan Hospital of Wuhan University admitted during March 2014 to October 2014 were enrolled in this study.All the tissues used in this experiment included 50 cases of breast cancer tissues and corresponding 50 cases of paracancer normal breast tissues with complete patients'information.The real-time quantitative polymerase chain reaction(qPCR)was applied to detect the expression of STC2 gene in 50 cases of breast cancer and paracancer normal breast tissues.The results showed that the expression level of STC2 gene in 50 cases of breast cancer tissues was significantly higher than that in paracancer normal breast tissues(P<0.001).The expression of STC2 gene was correlated with lymph node metastasis,distant metastasis,TNM stage and histological grade(P<0.001).The expression level of STC2 gene was significantly higher in breast cancer tissues with higher expression of Ki-67(P<0.001).The expression level of STC2 gene was significantly higher in estrogen receptor(ER)positive breast cancer tissues than in ER negative ones(P<0.001).However,different groups of age,pathological type,tumor size,PR expression and human epidermal growth factor receptor-2(HER2)expression did not show significant differences in STC2 expression(P>0.05).In conclusion,the abnormal overexpression of STC2 gene may play a role in the development and progression of breast cancer,and it can be used as an independent metastasis and prognostic factor of breast cancer.In addition,STC2 gene probably promotes the development and metastasis of breast cancer by interacting with estrogen and ER,and it may become a new direction for breast cancer endocrine therapy.

Effects of alanine glutamine on inflammatory, immune, antioxidant and nutritional indicators in colon cancer patients

Objective:To explore the effects of alanine glutamine on inflammatory, immune, antioxidant and nutritional indicators in colon cancer patients.Methods: A total of 126 cases of colon cancer with intestinal obstruction were divided into control group (n=63) and observation group (n=63) from May 2015 to May 2017 in Zhongnan Hospital of Wuhan University and Gezhouba Dam group central hospital, the control group only received parenteral nutrition, while the observation group was treated with parenteral nutrition plus alanine glutamine treatment. The expression levels of nutritional indicators, inflammatory factors, immunity and antioxidant were compared between the two groups.Results:Before and 1 d after treatment, there was no significant difference between the two groups of prealbumin (PA), albumin (ALB), tumor necrosis factor-α (TNF-α), C reactive protein (CRP), interferon-γ (IFN-γ) and immunoglobulin (IgA, IgG, IgM), superoxide dismutase (SOD) and malondialdehyde (MDA). 1 d after treatment, the levels of PA, ALB, IFN-γ, IgA, IgG, IgM and SOD in two groups were significantly lower than before the treatment, and TNF- , CRP and MDA were significantly higher than before the treatment. On the 7 d after treatment, the levels of PA, ALB, IFN-γ, IgA, IgG, IgM and SOD in two groups were significantly increased compared with the 1 d after treatment, while TNF-α, CRP and MDA were significantly lower than the 1 d after the treatment, the PA, ALB, IFN-γ, IgA, IgG, IgM and SOD levels in the observation group were significantly higher than those in the control group, while TNF-α, CRP and MDA were significantly lower than those of the control group, there was no significant difference in IgA, IgG and IgM levels between the observation group and that before treatment. There was no significant difference in MDA and SOD levels between the control group and that before tre atment.Conclusions: Parenteral nutrition support in patients with colon cancer with intestinal obstruction, alamyl glutamine, can significantly improve the nutritional level, reduce the inflammatory response, enhance the immune and antioxidant functions.