Sediment distribution and provenance since Late Pleistocene in Laizhou Bay,Bohai Sea,China

There are three transgression-regression events and evolutions of the sedimentary environment by sea level changes since the Pleistocene in the southern section of the Bohai Sea, China. It is obvious that a multi-source fluvial delta sedimentary system may be more dominant in a sedimentary environment. Based on previous research and survey or historical data, we carried out studies on the division of sedimentary units, sedimentary facies analysis and strata division and comparison, which aim to establish the sedimentary stratigraph of Laizhou Bay. We focus on the sedimentary procession of the Laizhou Bay since the early Late Pleistocene. It can be divided into two glacial periods and three interglacial periods, corresponding to two regression and three transgression events in Laizhou Bay since Late Pleistocene. In 124.6-72.0 ka BP, 60.0-24.4 ka BP and 10.2-4.0 ka BP, three times warm-wet periods occurred, respectively corresponding to the Cangzhou transgression, Xianxian transgression, and Huanghua transgression. In 72.0-60.0 ka BP and 24.4-10.2 ka BP, two dry and cold periods, it was the continental sedimentary environment, corresponding to Wurm early glacier and Wurm late glacier. The results show:(1) Sediments have the characteristics of phase and stage under the terrestrial input of the Yellow River and middle-small rivers in the southern section of the Bohai Sea.(2) PI moved towards coastal in Cangzhou transgression strata in early Late Pleistocene. PI moved northward from land in Xianxian transgression strata in the late Pleistocene. PI moved further north in the Huanghua transgression strata in Holocene.(3) During the regressive period, the land source input increased and the estuarine or lagoon sedimentation developed, which manifested as progradational superposition.(4) During the transgressive period, it mainly developed shallow coastal sediment and transitionally formed regressive deposition to the south in delta/tidal flat deposition.

Sediment distribution characteristics and environment evolution within 100 years in western Laizhou Bay,Bohai Sea,China

This study is about the reconstruction of fluvial origins based on the grain size distribution of sediment deposits in the western Laizhou Bay,Bohai Sea,China.Thirteen sediment cores were selected to research sediment characteristics using the Sahu discriminant formula,C-M diagram,and Folk method.The results showed:(1)Bounded by the Guangli River estuary,the north sediment was affected by the water and sand flowing from the Yellow River during different periods.The south sediment came from multi-source rivers under the influence of the Xiaoqing River,Mihe River,and other coastal rivers;(2)the deposited sediments were dated by a clear historical record of the branched channel oscillation combined with the characteristics of the diversion channel,erosion,and regression.The subaqueous delta overlapped during several Yellow River channel runs(1897–1904,1929–1934,1938–1947,1947–1953,1976–1996)and the deposited sediment facies changed(the north tidal flat-abandoned subaqueous delta-lateral delta-delta front);(3)the deposited sediment characteristics can be revealed by studying the branched diversions of the Yellow River and coastal multi-rivers of the past one hundred years.

Mutation of cytotoxin-associated gene A affects expressions of antioxidant proteins of Helicobacter pylori

AIM: To determine if disruption of the cagA gene of Helicobacter pylori (H pylori) has an effect on the expression of other proteins at proteome level. METHODS: Construction of a cagA knock out mutant Hp27 _△cagA (cagA -) via homologous recombination with the wild-type strain Hp27 (cagA+) as a recipient was performed. The method of sonication-urea-CHAPS-DTT was employed to extract bacterial proteins from both strains. Soluble proteins were analyzed by two-dimensional electrophoresis (2-DE). Images of 2-DE gels were digitalized and analyzed. Only spots that had a statistical signif icance in differential expression were selected and analyzed by matrix-assisted laser desorption/ionizationtime of flight mass spectrometry (MALDI-TOF-MS). Biological information was used to search protein database and identify the biological function of proteins. RESULTS: The proteome expressions between wild-type strain and isogenic mutant with the cagA gene knocked-out were compared. Five protein spots with high abundance in bacteria proteins of wild-type strains, down-regulated or absently expressed in bacteria proteins of mutants, were identified and analyzed. From a quantitative point of view, the identified proteins are related to the cagA gene and important antioxidant proteins of H pylori , including alkyl hydroperoxide reductase (Ahp), superoxide dismutase (SOD) and modulator of drug activity (Mda66), respectively, suggesting that cagA is important to maintain the normal activity of antioxidative stress and ensure H pylori persistent colonization in the host. CONCLUSION: cagA gene is relevant to the expressions of antioxidant proteins of H pylori, which may be a novel mechanism involved in H pylori cagA pathogenesis.

Chinese guidelines for the diagnosis and treatment of hand, foot and mouth disease (2018 edition)

Background Hand, foot, and mouth disease (HFMD) is a common infectious disease in childhood caused by an enterovirus (EV), and which is principally seen in children under 5 years of age. To promote diagnostic awareness and effective treat-ments, to further standardize and strengthen the clinical management and to reduce the mortality of HFMD, the guidelines for diagnosis and treatment have been developed. Methods National Health Commission of China assembled an expert committee for a revision of the guidelines. The com-mittee included 33 members who are specialized in diagnosis and treatment of HFMD. Results Early recognition of severe cases is utmost important in diagnosis and treatment of patients with HFMD. The key to diagnosis and treatment of severe cases lies in the timely and accurate recognition of stages 2 and 3 of HFMD, in order to stop progression to stage 4. Clinicians should particularly pay attention to those EV-A71 cases in children aged less than 3 years, and those with disease duration less than 3 days. The following indicators should alert the clinician of possible deterioration and impending critical disease: (1) persistent hyperthermia;(2) involvement of nervous system;(3) worsening respiratory rate and rhythm;(4) circulatory dysfunction;(5) elevated peripheral WBC count;(6) elevated blood glucose and (7) elevated blood lactic acid. For treatment, most mild cases can be treated as outpatients. Patients should be isolated to avoid cross-infection. Intense treatment modalities should be given for those severe cases. Conclusion The guidelines can provide systematic guidance on the diagnosis and management of HFMD.

A large outbreak of acute gastroenteritis caused by the human norovirus GII.17 strain at a university in Henan Province,China

Background:Human noroviruses are a major cause of viral gastroenteritis and are the main etiological agents of acute gastroenteritis outbreaks.An increasing number of outbreaks and sporadic cases of norovirus have been reported in China in recent years.There was a large acute gastroenteritis outbreak at a university in Henan Province,China in the past five years.We want to identify the source,transmission routes of the outbreak by epidemiological investigation and laboratory testing in order to provide the effective control measures.Methods:The clinical cases were investigated,and analysed by descriptive epidemiological methods according to factors such as time,department,grade and so on.Samples were collected from clinical cases,healthy persons,the environment,water,and food at the university.These samples were tested for potential bacteria and viruses.The samples that tested positive for norovirus were selected for whole genome sequencing and the sequences were then analysed.Results:From 4 March to 3 April 2015,a total of 753 acute diarrhoea cases were reported at the university;the attack rate was 3.29%.The epidemic curve showed two peaks,with the main peak occurring between 10 and 20 March,accounting for 85.26%of reported cases.The rates of norovirus detection in samples from confirmed cases,people without symptoms,and environmental samples were 32.72%,17.39%,and 9.17%,respectively.The phylogenetic analysis showed that the norovirus belonged to the genotype GII.17.Conclusions:This is the largest and most severe outbreak caused by genotype GII.17 norovirus in recent years in China.The GII.17 viruses displayed high epidemic activity and have become a dominant strain in China since the winter of 2014,having replaced the previously dominant GII.4 Sydney 2012 strain.

Prevalence of severe fever with thrombocytopenia syndrome virus in animals in Henan Province, China

Severe fever with thrombocytopenia syndrome(SFTS)is an emerging infectious disease caused by severe fever with thrombocytopenia syndrome virus(SFTSV).SFTSV has been found in humans,ticks and animals,and SFTS has high mortality and increasing prevalence in East Asia.In the study,the samples(heart,liver,lung,kidney,spleen,brain tissue and serum)were collected from 374 domestic animals and 241 wild animals in Pingqiao District and Xinxian County of Xinyang in Henan Province,China.275(44.72%,275/615)animals were positive for anti-SFTSV antibodies,the anti-SFTSV antibodies positive ratios of domestic and wild animals were 43.58%(163/374)and 46.47%(112/241),respectively.There was no significant difference in domestic and wild animals,but significant differences were detected among different species of animals(x^2=112.59,P<0.0001).Among 615 animals,105(17.07%,105/615)animals were positive for SFTSV RNA,and only one SFTSV strain was isolated from heart tissue of a yellow weasel.The phylogenetic analysis shows that the sequence from animals belonged to the same group with viral sequences obtained from humans.The animals maybe play a reservoir host in maintaining the life cycle of SFTSV in nature.

Bites from the same dog,different outcomes for two patients:a case report

Background:Rabies is a serious reemerging zoonosis in China.At present human rabies cases are primarily diagnosed based on clinical presentation.Case presentation:In August 2012,a woman and her son were attacked by a stray dog in Henan,China.The son received rabies postexposure prophylaxis(wound treatment followed by vaccine,no immunoglobulin),however,the mother did not.Rabies infection was subsequently laboratory confirmed in the mother and she died in December;her son is alive and healthy after 2 years of follow-up.Conclusion:This report documents that the timely utilization of postexposure prophylaxis is a required measure in preventing rabies after exposure to an animal bite.