CRISPR-detector:fast and accurate detection,visualization,and annotation of genome-wide mutations induced by genome editing events

The leading-edge CRISPR/CRISPR-associated technology is revolutionizing biotechnologies through genome editing.To track on/off-target events with emerging new editing techniques,improved bioinformatic tools are indispensable.Existing tools suffer from limitations in speed and scalability,especially with whole-genome sequencing(WGS)data analysis.To address these limitations,we have developed a comprehensive tool called CRISPR-detector,a web-based and locally deployable pipeline for genome editing sequence analysis.The core analysis module of CRISPR-detector is based on the Sentieon TNscope pipeline,with additional novel annotation and visualization modules designed to fit CRISPR applications.Co-analysis of the treated and control samples is performed to remove existing background variants prior to genome editing.CRISPR-detector offers optimized scalability,enabling WGS data analysis beyond Browser Extensible Data file-defined regions,with improved accuracy due to haplotype-based variant calling to handle sequencing errors.In addition,the tool also provides integrated structural variation calling and includes functional and clinical annotations of editing-induced mutations appreciated by users.These advantages facilitate rapid and efficient detection of mutations induced by genome editing events,especially for datasets generated from WGS.The web-based version of CRISPR-detector is available at https://db.cngb.org/crispr-detector,and the locally deployable version is available at https://github.com/hlcas/CRISPR-detector.

Corrigendum to“CRISPR-detector:fast and accurate detection,visualization,and annotation of genome-wide mutations induced by genome editing events”[Journal of Genetics and Genomics(2023)563-572]

This corrigendum clarifies information in the article“CRISPR-detector:fast and accurate detection,visualization,and annotation of genomewide mutations induced by genome editing events”by Huang et al.(2023).In the figure legend of Fig.S1,the sentence“CRISPR-detector failed to report insertions larger than 72 bp while CRISPResso2 failed to report insertions larger than 53 bp.”should be corrected into“CRISPR-detector failed to report deletions larger than 71 bp while CRISPResso2 failed to report deletions larger than 53 bp.”