Ultraviolet blood irradiation has been used as a physical therapy to treat many nonspeci¯c diseases in clinics;however,the underlying mechanisms remain largely unclear.Neutrophils,the first line of host defense,p...Ultraviolet blood irradiation has been used as a physical therapy to treat many nonspeci¯c diseases in clinics;however,the underlying mechanisms remain largely unclear.Neutrophils,the first line of host defense,play a crucial role in a variety of in°ammatory responses.In the present work,we investigated the effects of ultraviolet light A(UVA)on the immune functions of human neutrophils at the single-cell level by using an inverted°uorescence microscope.N-Formylmethionyl-leucyl-phenylalanine(FMLP),a classic physiological chemotactic peptide,was used to induce a series of immune responses in neutrophils in vitro.FMLP-induced calcium mobilization,migration,and phagocytosis in human neutrophils was significantly blocked after treatment with 365 nm UVA irradiation,demonstrating the immunosuppressive effects of UVA irradiation on neutrophils.Similar responses were also observed when the cells were pretreated with H2O2,a type of reactive oxygen species(ROS).Furthermore,UVA irradiation resulted in an increase in NAD(P)H,a member of host oxidative stress in cells.Taken together,our data indicate that UVA irradiation results in immunosuppression associated with the production of ROS in human neutrophils.展开更多
Epithelial cells can assemble into cohesive monolayers with rich morphologies on substrates due to competition between elastic,edge,and interfacial effects.Here we present a molecularly based thermodynamic model,integ...Epithelial cells can assemble into cohesive monolayers with rich morphologies on substrates due to competition between elastic,edge,and interfacial effects.Here we present a molecularly based thermodynamic model,integrating monolayer and substrate elasticity,and force-mediated focal adhesion formation,to elucidate the active biochemical regulation over the cellular force landscapes in cohesive epithelial monolayers,corroborated by microscopy and immunofluorescence studies.The predicted extracellular traction and intercellular tension are both monolayer size and substrate stiffness dependent,suggestive of cross-talks between intercellular and extracellular activities.Our model sets a firm ground toward a versatile computational framework to uncover the molecular origins of morphogenesis and disease in multicellular epithelia.展开更多
基金This work was supported by the National Natural Science Foundation of China(No.11204142),the National Basic Research Program of China (No.2013CB328702)International S&T Cooperation Program of China (2011DFA52870)+1 种基金the 111 Project(No.B07013),the National Science Fund for Talent Training in Basic Sciences(No.J1103208),the PCSIRT (IRT0149)the Social Development Fund of Ge'ermu Science and Technology Bureau and the State Key Laboratory of Medicinal Chemical Biology.
文摘Ultraviolet blood irradiation has been used as a physical therapy to treat many nonspeci¯c diseases in clinics;however,the underlying mechanisms remain largely unclear.Neutrophils,the first line of host defense,play a crucial role in a variety of in°ammatory responses.In the present work,we investigated the effects of ultraviolet light A(UVA)on the immune functions of human neutrophils at the single-cell level by using an inverted°uorescence microscope.N-Formylmethionyl-leucyl-phenylalanine(FMLP),a classic physiological chemotactic peptide,was used to induce a series of immune responses in neutrophils in vitro.FMLP-induced calcium mobilization,migration,and phagocytosis in human neutrophils was significantly blocked after treatment with 365 nm UVA irradiation,demonstrating the immunosuppressive effects of UVA irradiation on neutrophils.Similar responses were also observed when the cells were pretreated with H2O2,a type of reactive oxygen species(ROS).Furthermore,UVA irradiation resulted in an increase in NAD(P)H,a member of host oxidative stress in cells.Taken together,our data indicate that UVA irradiation results in immunosuppression associated with the production of ROS in human neutrophils.
基金S.L.Z.acknowledges support by the National Science Foundation(NSF)under Grants CMMI-0754463 and CBET-1067523by the National Institute of Health(NIH)under the grant R21HL122902.
文摘Epithelial cells can assemble into cohesive monolayers with rich morphologies on substrates due to competition between elastic,edge,and interfacial effects.Here we present a molecularly based thermodynamic model,integrating monolayer and substrate elasticity,and force-mediated focal adhesion formation,to elucidate the active biochemical regulation over the cellular force landscapes in cohesive epithelial monolayers,corroborated by microscopy and immunofluorescence studies.The predicted extracellular traction and intercellular tension are both monolayer size and substrate stiffness dependent,suggestive of cross-talks between intercellular and extracellular activities.Our model sets a firm ground toward a versatile computational framework to uncover the molecular origins of morphogenesis and disease in multicellular epithelia.
