Alternative splicing is a tightly regulated process that contributes to cancer development.CRNDE is a long noncoding RNA with alternative splicing and is implicated in the pathogenesis of several cancers.However,wheth...Alternative splicing is a tightly regulated process that contributes to cancer development.CRNDE is a long noncoding RNA with alternative splicing and is implicated in the pathogenesis of several cancers.However,whether deregulated expression of CRNDE is common and which isoforms are mainly involved in cancers remain unclear.In this study,we report that CRNDE is aberrantly expressed in the majority of solid and hematopoietic malignancies.The investigation of CRNDE expression in normal samples revealed that CRNDE was expressed in a tissue- and cell-specific manner.Further comparison of CRNDE expression in 2938 patient samples from 15 solid and hematopoietic tumors showed that CRNDE was significantly overexpressed in 11 malignancies,including 3 reported and 8 unreported,and also implicated that the overexpressed isoforms differed in various cancer types.Furthermore,anti-cancer drugs could efficiently repress CRNDE overexpression in cancer cell lines and primary samples,and even had different impacts on the expression of CRNDE isoforms.Finally,experimental profiles of 12 alternatively spliced isoforms demonstrated that the spliced variant CRNDE-g was the most highly expressed isoform in multiple cancer types.Collectively,our results emphasize the cancer-associated feature of CRNDE and its spliced isoforms,and may provide promising targets for cancer diagnosis and therapy.展开更多
A low-pressure reactor(LPR) was developed for the measurement of ambient organic peroxy(RO2)radicals with the use of the laser-induced fluorescence(LIF) instrument.The reactor converts all the RO_(x)(=RO2+HO2+RO+OH) r...A low-pressure reactor(LPR) was developed for the measurement of ambient organic peroxy(RO2)radicals with the use of the laser-induced fluorescence(LIF) instrument.The reactor converts all the RO_(x)(=RO2+HO2+RO+OH) radicals into HO2 radicals.It can conduct different measurement modes through altering the reagent gases,achieving the speciated measurement of RO2 and RO2^#(RO2 radicals derived from the long-chain alkane,alkene and aromatic hydrocarbon).An example of field measurement results was given,with a maximum concentration of 1.88 × 10^(8) molecule/cm^(3) for RO2 and 1.18×10^(8) molecule/cm^(3) for RO2^(#).Also,this instrument quantifies the local ozone production rates directly,which can help to deduce the regional ozone control strategy from an experimental perspective.The new device can se rve as a potent tool for both the explo ration of frontier chemistry and the diagnosis of the control strategies.展开更多
基金National Natural Science Foundation of China (Nos.81530003,81300403,81770153 and 91440114)The National Key Research and Development Program (No.2016YFC0902800)+1 种基金Shanghai Leading Talent Projects (No.2015008)the Academic Leader Program of Shanghai Science and Technology Committee (No.2015137).
文摘Alternative splicing is a tightly regulated process that contributes to cancer development.CRNDE is a long noncoding RNA with alternative splicing and is implicated in the pathogenesis of several cancers.However,whether deregulated expression of CRNDE is common and which isoforms are mainly involved in cancers remain unclear.In this study,we report that CRNDE is aberrantly expressed in the majority of solid and hematopoietic malignancies.The investigation of CRNDE expression in normal samples revealed that CRNDE was expressed in a tissue- and cell-specific manner.Further comparison of CRNDE expression in 2938 patient samples from 15 solid and hematopoietic tumors showed that CRNDE was significantly overexpressed in 11 malignancies,including 3 reported and 8 unreported,and also implicated that the overexpressed isoforms differed in various cancer types.Furthermore,anti-cancer drugs could efficiently repress CRNDE overexpression in cancer cell lines and primary samples,and even had different impacts on the expression of CRNDE isoforms.Finally,experimental profiles of 12 alternatively spliced isoforms demonstrated that the spliced variant CRNDE-g was the most highly expressed isoform in multiple cancer types.Collectively,our results emphasize the cancer-associated feature of CRNDE and its spliced isoforms,and may provide promising targets for cancer diagnosis and therapy.
基金supported by the National Natural Science Foundation of China(21976006,41907185,91844301,91544225)Beijing Natural Science Foundation,China(JQ19031)China Postdoctoral Science Foundation(2018M641095,2019T120023)。
基金the National Key R&D Program of China(No.2017YFC0209402)the Beijing Natural Science Foundation,China(No.JQ19031)。
文摘A low-pressure reactor(LPR) was developed for the measurement of ambient organic peroxy(RO2)radicals with the use of the laser-induced fluorescence(LIF) instrument.The reactor converts all the RO_(x)(=RO2+HO2+RO+OH) radicals into HO2 radicals.It can conduct different measurement modes through altering the reagent gases,achieving the speciated measurement of RO2 and RO2^#(RO2 radicals derived from the long-chain alkane,alkene and aromatic hydrocarbon).An example of field measurement results was given,with a maximum concentration of 1.88 × 10^(8) molecule/cm^(3) for RO2 and 1.18×10^(8) molecule/cm^(3) for RO2^(#).Also,this instrument quantifies the local ozone production rates directly,which can help to deduce the regional ozone control strategy from an experimental perspective.The new device can se rve as a potent tool for both the explo ration of frontier chemistry and the diagnosis of the control strategies.
