Sterically spacing and locating functional matters at the nanoscale exert critical effects on their application,especially for the fluorescence probes whose aggregation causes emission quenching.Here we achieved a hie...Sterically spacing and locating functional matters at the nanoscale exert critical effects on their application,especially for the fluorescence probes whose aggregation causes emission quenching.Here we achieved a hierarchical spacing strategy of DNA fluorescence probes for ion detection via locating them separately on rod-like cellulose nanocrystals(CNCs)and further isolating CNCs by pre-grafting long molecular chains.Controlling chemical structure of CNC and location degree could adjust the interspace of DNA probes(with a molecular length of ca.3.6 nm)in a range of 3.5-6.5 nm with a gradient about 0.2 nm.A length up to micrometer scale of the CNC nanorods was necessary to provide DNA probes with well-separated grafting locations and enough freedom,which brought a vast linear detection range from 10 nmol/L to 5 μmol/L of Hg^2+ concentration.The abundant reactive sites on CNC allowed a grafting pre-location of poly(tert-butyl acrylate)(PtBA)to promote the isolation of DNA probes.Controlled radical polymerization was employed to adj ust the length of PtBA molecular chains,which increased the linear sensitivity coefficient of Hg^2+ detection by ca.2.5 times.This hierarchical nanoscale spacing concept based on chemical design can hopefully cond uce to the development of biosensor and medical diagnosis.A hierarchical spacing strategy was applied to separate DNA fluorescent probes on CNCs and detect ion concentration linearly.The first-level spacing was to locate probes uniformly on CNCs,obtaining a wide linear range;and the second-level spacing was to isolate CNCs with polymer,obtaining an increased linear coefficient.展开更多
基金financially supported by the National Natural Science Foundation of China (51603171 and 51373131)Talent Project of Southwest University (SWU115034)+2 种基金Fundamental Research Funds for the Central Universities (XDJK2016C032)Key Laboratory of Polymeric Composite & Functional Materials of Ministry of Education (PCFM201605)Hubei Key Laboratory of Advanced Textile Materials & Application (Fzxcl2017003)
文摘Sterically spacing and locating functional matters at the nanoscale exert critical effects on their application,especially for the fluorescence probes whose aggregation causes emission quenching.Here we achieved a hierarchical spacing strategy of DNA fluorescence probes for ion detection via locating them separately on rod-like cellulose nanocrystals(CNCs)and further isolating CNCs by pre-grafting long molecular chains.Controlling chemical structure of CNC and location degree could adjust the interspace of DNA probes(with a molecular length of ca.3.6 nm)in a range of 3.5-6.5 nm with a gradient about 0.2 nm.A length up to micrometer scale of the CNC nanorods was necessary to provide DNA probes with well-separated grafting locations and enough freedom,which brought a vast linear detection range from 10 nmol/L to 5 μmol/L of Hg^2+ concentration.The abundant reactive sites on CNC allowed a grafting pre-location of poly(tert-butyl acrylate)(PtBA)to promote the isolation of DNA probes.Controlled radical polymerization was employed to adj ust the length of PtBA molecular chains,which increased the linear sensitivity coefficient of Hg^2+ detection by ca.2.5 times.This hierarchical nanoscale spacing concept based on chemical design can hopefully cond uce to the development of biosensor and medical diagnosis.A hierarchical spacing strategy was applied to separate DNA fluorescent probes on CNCs and detect ion concentration linearly.The first-level spacing was to locate probes uniformly on CNCs,obtaining a wide linear range;and the second-level spacing was to isolate CNCs with polymer,obtaining an increased linear coefficient.
