背景与目的目前在单孔胸腔镜肺手术中需留置双根胸腔引流管时常联合使用细管与粗管,且粗管多置于切口内,增加了术后疼痛感及切口愈合不良风险。本研究将评估单孔胸腔镜肺部术后采用双根10 F超细猪尾巴管引流的疗效及安全性。方法回顾苏...背景与目的目前在单孔胸腔镜肺手术中需留置双根胸腔引流管时常联合使用细管与粗管,且粗管多置于切口内,增加了术后疼痛感及切口愈合不良风险。本研究将评估单孔胸腔镜肺部术后采用双根10 F超细猪尾巴管引流的疗效及安全性。方法回顾苏州大学附属第二医院胸心外科同一治疗组2018年6月-2020年6月的单孔胸腔镜肺手术病历资料,对比在不同时期分别采用“10 F超细猪尾巴管+24 F粗管”及“双根10 F超细猪尾巴管”两种胸腔引流方案的效果。结果2019年6月及以后采用“双根10 F超细猪尾巴管”方案的A组共有106例,2019年6月及以前采用10 F超细猪尾巴管+24 F粗管的B组共有183例。术后A、B两组胸腔引流液量(mL)(1^(st):199.54±126.56 vs 203.59±139.32,P=0.84;2^(nd):340.30±205.47 vs 349.74±230.92,P=0.76;3^(rd):435.19±311.51 vs 451.37±317.03,P=0.70;4^(th):492.58±377.33 vs 512.57±382.94,P=0.69;共计:604.57±547.24 vs 614.64±546.08,P=0.88)、引流管留置时间(d)(上管:2.54±2.20 vs 3.40±2.07,P=0.21;下管:2.24±2.43 vs 3.82±2.12,P=0.10)、术后住院时间(d)(6.87±3.17 vs 7.06±3.21,P=0.63)、切口愈合不良情况(0 vs 3.28%,P=0.09)、术后调整下胸腔引流管情况(0.94%vs 2.19%,P=0.66)、术后第一次视觉模拟量表(visual analogue score,VAS)(3.00±0.24 vs 2.99±0.15,P=0.63)在两组之间均无统计学差异。但A组术后VAS2(2.28±0.63 vs 2.92±0.59,P<0.01)、VAS3(2.50±1.58 vs 2.79±1.53,P=0.02)、术后追加镇痛药物频次(25.47%vs 38.25%,P=0.03)及术后调整上胸腔引流管的频次(0 vs 4.37%,P=0.03)均较B组显著偏低。结论在部分高选择的单孔胸腔镜肺手术过程中采用双根10 F超细猪尾巴管引流安全有效,可减少术后疼痛,降低术后胸腔引流管重置发生率。展开更多
In this paper,single-factor experiments and orthogonal experiment were conducted to optimize the water bath extraction of polyphenols from Qing Brick Tea,and the optimal extraction conditions obtained were as follows:...In this paper,single-factor experiments and orthogonal experiment were conducted to optimize the water bath extraction of polyphenols from Qing Brick Tea,and the optimal extraction conditions obtained were as follows:solvent of 50%ethanol,solid/liquid ratio of 1∶30,temperature of 80℃,and time of 6 min.Under the optimal water bath extraction conditions,polyphenols in fresh tea leaves,fresh tea petioles,fresh tea blades,finished brick tea,finished tea petioles,finished tea blades,tea dregs after brewing and tea dregs after boiling were extracted,respectively.It was found that the quality fraction of polyphenols extracted from fresh tea leaves was the highest,with the best extraction effect.The extraction effect of leaf blades was better than that of leaf petioles,and the extraction effect of tea dregs after brewing was better than that of tea dregs after boiling.展开更多
Ischemia-reperfusion (IR) injury represents a major cause of myocardial dysfunction after infarction and thrombolytic therapy, and it is closely related to the free radical explosion and overwhelming inflammatory resp...Ischemia-reperfusion (IR) injury represents a major cause of myocardial dysfunction after infarction and thrombolytic therapy, and it is closely related to the free radical explosion and overwhelming inflammatory responses. Herein, macrophage-targeting nanocomplexes (NCs) are developed to mediate efficient co-delivery of siRNA against MOF (siMOF) and microRNA-21 (miR21) into myocardial macrophages, cooperatively orches-trating the myocardial microenvironment against IR injury. Bioreducible, branched poly(β-amino ester) (BPAE-SS) is designed to co-condense siMOF and miR21 into NCs in a multivalency-reinforced approach, and they are surface-decorated with carboxylated mannan (Man-COOH) to shield the positive surface charges and enhance the serum stability. The final MBSsm NCs are efficiently internalized by myocardial macrophages after systemic administration, wherein BPAE-SS is degraded into small segments by intracellular glutathione to promote the siMOF/miR21 release, finally provoking efficient gene silencing. Thus, cardiomyocyte protection and macro-phage modulation are realized via the combined effects of ROS scavenging, inflammation inhibition, and autophagy attenuation, which ameliorates the myocardial microenvironment and restores the cardiac function via positive cellular crosstalk. This study renders promising solutions to address the multiple systemic barriers against in vivo nucleic acid delivery, and it also offers new options for IR injury by manipulating multiple reciprocal bio-reactions.展开更多
文摘背景与目的目前在单孔胸腔镜肺手术中需留置双根胸腔引流管时常联合使用细管与粗管,且粗管多置于切口内,增加了术后疼痛感及切口愈合不良风险。本研究将评估单孔胸腔镜肺部术后采用双根10 F超细猪尾巴管引流的疗效及安全性。方法回顾苏州大学附属第二医院胸心外科同一治疗组2018年6月-2020年6月的单孔胸腔镜肺手术病历资料,对比在不同时期分别采用“10 F超细猪尾巴管+24 F粗管”及“双根10 F超细猪尾巴管”两种胸腔引流方案的效果。结果2019年6月及以后采用“双根10 F超细猪尾巴管”方案的A组共有106例,2019年6月及以前采用10 F超细猪尾巴管+24 F粗管的B组共有183例。术后A、B两组胸腔引流液量(mL)(1^(st):199.54±126.56 vs 203.59±139.32,P=0.84;2^(nd):340.30±205.47 vs 349.74±230.92,P=0.76;3^(rd):435.19±311.51 vs 451.37±317.03,P=0.70;4^(th):492.58±377.33 vs 512.57±382.94,P=0.69;共计:604.57±547.24 vs 614.64±546.08,P=0.88)、引流管留置时间(d)(上管:2.54±2.20 vs 3.40±2.07,P=0.21;下管:2.24±2.43 vs 3.82±2.12,P=0.10)、术后住院时间(d)(6.87±3.17 vs 7.06±3.21,P=0.63)、切口愈合不良情况(0 vs 3.28%,P=0.09)、术后调整下胸腔引流管情况(0.94%vs 2.19%,P=0.66)、术后第一次视觉模拟量表(visual analogue score,VAS)(3.00±0.24 vs 2.99±0.15,P=0.63)在两组之间均无统计学差异。但A组术后VAS2(2.28±0.63 vs 2.92±0.59,P<0.01)、VAS3(2.50±1.58 vs 2.79±1.53,P=0.02)、术后追加镇痛药物频次(25.47%vs 38.25%,P=0.03)及术后调整上胸腔引流管的频次(0 vs 4.37%,P=0.03)均较B组显著偏低。结论在部分高选择的单孔胸腔镜肺手术过程中采用双根10 F超细猪尾巴管引流安全有效,可减少术后疼痛,降低术后胸腔引流管重置发生率。
基金Key Project of Philosophy and Social Science Research in Colleges and Universities of Hubei Province(18ZD030)Cultivation Project of Hubei University of Science and Technology(2020-21X12)Open Fund of Enan Cultural Research Center,Hubei Provincial Key Research Base of Humanities and Social Sciences(2018-19XZE04)。
文摘In this paper,single-factor experiments and orthogonal experiment were conducted to optimize the water bath extraction of polyphenols from Qing Brick Tea,and the optimal extraction conditions obtained were as follows:solvent of 50%ethanol,solid/liquid ratio of 1∶30,temperature of 80℃,and time of 6 min.Under the optimal water bath extraction conditions,polyphenols in fresh tea leaves,fresh tea petioles,fresh tea blades,finished brick tea,finished tea petioles,finished tea blades,tea dregs after brewing and tea dregs after boiling were extracted,respectively.It was found that the quality fraction of polyphenols extracted from fresh tea leaves was the highest,with the best extraction effect.The extraction effect of leaf blades was better than that of leaf petioles,and the extraction effect of tea dregs after brewing was better than that of tea dregs after boiling.
基金This work was supported by the National Natural Science Foundation of China(82172076,51873142,and 52033006)Jiangsu Key Research and Development Plan(Social Development)Project(BE2020653 and BE2021642)+1 种基金Suzhou Science and Technology Development Project(SYS2019072)Collaborative Innovation Center of Suzhou Nano Science&Technology,the 111 project,Suzhou Key Laboratory of Nanotech-nology and Biomedicine,and Joint International Research Laboratory of Carbon-Based Functional Materials and Devices.
文摘Ischemia-reperfusion (IR) injury represents a major cause of myocardial dysfunction after infarction and thrombolytic therapy, and it is closely related to the free radical explosion and overwhelming inflammatory responses. Herein, macrophage-targeting nanocomplexes (NCs) are developed to mediate efficient co-delivery of siRNA against MOF (siMOF) and microRNA-21 (miR21) into myocardial macrophages, cooperatively orches-trating the myocardial microenvironment against IR injury. Bioreducible, branched poly(β-amino ester) (BPAE-SS) is designed to co-condense siMOF and miR21 into NCs in a multivalency-reinforced approach, and they are surface-decorated with carboxylated mannan (Man-COOH) to shield the positive surface charges and enhance the serum stability. The final MBSsm NCs are efficiently internalized by myocardial macrophages after systemic administration, wherein BPAE-SS is degraded into small segments by intracellular glutathione to promote the siMOF/miR21 release, finally provoking efficient gene silencing. Thus, cardiomyocyte protection and macro-phage modulation are realized via the combined effects of ROS scavenging, inflammation inhibition, and autophagy attenuation, which ameliorates the myocardial microenvironment and restores the cardiac function via positive cellular crosstalk. This study renders promising solutions to address the multiple systemic barriers against in vivo nucleic acid delivery, and it also offers new options for IR injury by manipulating multiple reciprocal bio-reactions.
