Construction of Large Human Single-chain Antibody Phage Display Library

A large human naive single chain antibody (scFv) library is constructed from 60 healthy donors via phage display technique. During the period, some methods are employed to optimize the diversity, such as multi donors, different annealing temperature, half nest PCR, and assembly by two way fusion PCR. In this study, 78 electroporations resulted in 1010 library, diversity of which is assayed by enzyme fingerprint. The efficiency and diversity are all better than other researches.

玉米倒伏对机械粒收损失的影响及倒伏减损收获技术

通过调查玉米田间倒伏率与机械粒收产量损失率,建立倒伏率与机械粒收损失率之间的量化关系,采取改变割台高度和收获方向等措施,明确不同收获方式对产量损失的影响。结果表明,倒伏对玉米产量影响主要表现为落穗损失。与根倒相比,茎折对玉米产量损失的影响更大,茎折每增加1%,落穗率增加0.28%;根倒每增加1%,落穗率增加0.17%。茎折发生的部位越低,落穗率越高。收获时降低收获机割台可以减少部分产量损失,易造成收获机割台堵塞,收获速度和效率大幅下降。与顺倒伏方向和垂直倒伏方向收获相比,逆倒伏方向收获的落穗损失最低。