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Three-dimensional reconstruction of Picea wilsonii Mast. pollen grains using automated electron microscopy 被引量:4
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作者 Weiwei Shen Lingyu Ma +7 位作者 Xi Zhang Xixia Li Yuanyuan Zhao Yanping Jing Yun Feng xueke tan Fei Sun Jinxing Lin 《Science China(Life Sciences)》 SCIE CAS CSCD 2020年第2期171-179,共9页
Three-dimensional electron microscopy(3 D-EM) has attracted considerable attention because of its ability to provide detailed information with respect to developmental analysis. However, large-scale high-resolution 3 ... Three-dimensional electron microscopy(3 D-EM) has attracted considerable attention because of its ability to provide detailed information with respect to developmental analysis. However, large-scale high-resolution 3 D reconstruction of biological samples remains challenging. Herein, we present a 3 D view of a Picea wilsonii Mast. pollen grain with 100 nm axial and38.57 nm lateral resolution using AutoCUTS-SEM(automatic collector of ultrathin sections-scanning electron microscopy). We established a library of 3,127 100 nm thick serial sections of pollen grains for preservation and observation, demonstrating that the protocol can be used to analyze large-volume samples. After obtaining the SEM images, we reconstructed an entire pollen grain comprising 734 serial sections. The images produced by 3D reconstruction clearly revealed the main components of the P.wilsonii pollen grain, i.e., two sacci and pollen corpus, tube cell, generative cell, and two degenerated prothallial cells, and their internal organization. In addition, we performed a quantitative analysis of the different pollen grain cells, including sacci, and found that there were 202 connections within a saccus SEM image. Thus, for the first time, this study provided a global 3D view of the entire pollen grain, which will be useful for analyzing pollen development and growth. 展开更多
关键词 3D RECONSTRUCTION AutoCUTS-SEM ELECTRON MICROSCOPY POLLEN
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